Abstract: The present disclosure relates in some aspects to recombinant polymerases, variant PumA1 polymerases, and compositions thereof. Also provided herein are methods of using the recombinant polymerases and/or variant PumA1 polymerases for nucleic acid amplification (e.g., rolling circle amplification). In some aspects, the compositions and methods disclosed herein provide more robust amplification (e.g., RCA) reactions for improved in vitro and in situ analysis.

Abstract: The present disclosure provides a method for identifying modal parameters of engineering structures based on fast stochastic subspace identification, and relates to the field of structural modal parameters identification. The method includes the following steps: collecting responses; obtaining a unitary matrix by performing random projection on and QR decomposition of a matrix; obtaining a small matrix by projecting a Toeplitz matrix onto the unitary matrix; obtaining U, S, V matrices respectively by performing singular value decomposition of the small matrix; performing eigenvalue decomposition; and determining an order interval. According to the present disclosure, the small matrix is obtained through performing random projection on and QR decomposition of the traditional Toeplitz matrix, the dimensionality of the matrix by singular value decomposition is reduced and the computational efficiency of the matrix is improved.

Abstract: The present disclosure relates in some aspects to methods for analyzing a target RNA in a biological sample, such as detection of a sequence of interest in an RNA. In some aspects, provided herein are methods of using an RNase such as RNase H to provide in situ RNA detection methods having high sensitivity, efficiency, and/or specificity.

Abstract: An anti-static globe valve for hyperbaric oxygen and a method are provided, belong to the technical field of oxygen globe valves. The globe valve comprises a valve body, a valve disc, a valve seat and a valve stem. The valve seat is installed in the valve body, and the valve disc moves in an air channel of the valve body. When the valve disc is in contact with the valve seat, the valve disc is able to cut off the flowing of a medium in the air channel. When the valve disc is far away from the valve seat, the flowing of the medium in the air channel is allowed. An upper sleeve and a lower sleeve are further provided in the valve body, the upper sleeve is perpendicular to and in communication with the air channel, and the valve disc moves up and down in the upper sleeve.

Abstract: The utility model discloses a cutting assembly for a vegetable cutter and a hand-held electric vegetable cutter, which falls within the field of vegetable cutting appliances. The cutting assembly for a vegetable cutter of the utility model comprises a cup body and a cutting disc, wherein the cutting disc is axially mounted in a cutter disc mounting cavity by an upper end of the cup body, the outer periphery of the cutting disc is supported on a limiting step, and a rotation connecting portion is provided at the center of the cutting disc so that the cutting disc can axially rotate in the cup body.

Abstract: The present invention provides methods, compositions, and systems for distributing molecules and complexes into reaction sites. In particular, the methods, compositions, and systems of the present invention result in an active loading of molecules and complexes into reaction sites with improved efficiency over loading by passive diffusion methods alone.

Abstract: A method of natural gas treatment including introducing a natural gas containing stream into a dryer unit, thereby producing a treated natural gas containing stream. Introducing the treated natural gas containing stream into a nitrogen rejection unit, thereby producing a further treated natural gas stream as a nitrogen rejection unit product. Splitting the nitrogen rejection unit product into at least two portions, introducing the first portion of the further treated natural gas stream into a reformer unit as first part of feed, and introducing a second portion of the further treated natural gas stream into the dryer unit as a regeneration stream, thereby producing a regeneration waste stream. Introducing at least a portion of the regeneration waste stream into the reformer unit as second part of feed.

Abstract: A method of maintaining a syngas composition ratio during an upset condition, including detecting a reduction in the import carbon dioxide flow rate with a carbon dioxide import stream flow sensor, evaluating the reduction in carbon dioxide flow rate or carbon dioxide pressure in a controller, performing one or more predetermined corrective actions as instructed by the controller. Wherein the predetermined corrective actions are chosen from the following: opening a CO2 import stream flow valve, opening a hydrocarbon and steam stream feed valve, opening a CO2 backup stream control valve, opening a syngas backup letdown valve, and starting a composition adjustment unit.

Abstract: A method of natural gas treatment including introducing a natural gas containing stream into a dryer unit, thereby producing a treated natural gas containing stream. Introducing the treated natural gas containing stream into a nitrogen rejection unit, thereby producing a further treated natural gas stream as a nitrogen rejection unit product. Splitting the nitrogen rejection unit product into at least two portions, introducing the first portion of the further treated natural gas stream into a reformer unit as first part of feed, and introducing a second portion of the further treated natural gas stream into the dryer unit as a regeneration stream, thereby producing a regeneration waste stream. Introducing at least a portion of the regeneration waste stream into the reformer unit as second part of feed.

Abstract: Disclosed is a safe hand-pulled vegetable chopper, which relates to the field of food processing tools. The vegetable chopper herein includes a cup body, a lid matched with the cup body, a puller cord driving member in the lid, and a blade assembly in the cup body and detachably connected with a driving end of the puller cord driving member, a circular skirt-like enclosure is provided from a bottom of the lid around an outer periphery of the blade assembly for shielding the blade assembly, the skirt-like enclosure is on an inner side of the cup body when the cup body engages the lid, and the blade assembly stays on the lid when the cup body and the lid are separated.

Abstract: The present invention provides methods, compositions, and systems for distributing molecules and complexes into reaction sites. In particular, the methods, compositions, and systems of the present invention result in an active loading of molecules and complexes into reaction sites with improved efficiency over loading by passive diffusion methods alone.

Abstract: A request to initiate startup of an air separation plant may be received, and, in response to receiving the request, startup information that identifies a sequence of steps to be automatically executed to start up the air separation plant is retrieved. Each step may be associated with a component of the air separation plant, and may be associated with an action and a set of permissives corresponding to the action. The set of permissives for each action may specify one or more parameters for controlling the execution of the corresponding action. After retrieving the startup information, the system may automatically initiate execution of the sequence of steps, and may monitor the execution of each of the steps. The system may determine, based on the monitoring, whether to modify a parameter specified by one of the permissives corresponding to an executed action.

Abstract: Methods, compositions, and systems for distributing nucleic acids into array regions are provided. The methods, compositions, and systems utilize nucleic acid condensing agents to increase efficiency of distribution of the nucleic acids into the array regions. Various methods for facilitating distribution of the nucleic acids to the array regions are provided.