Abstract: A two-photon stimulated emission depletion composite microscope using continuous light loss, the microscope comprising: a two-photon imaging unit (100) and an STED imaging unit (200), wherein for thicker samples, the two-photon imaging unit (100) can be used, and for regions of interest on the surface of samples, the STED super-resolution imaging unit (200) can be used. The two-photon stimulated emission depletion composite microscope using continuous light loss integrates two functions of STED imaging and two-photon imaging, so as to provide a powerful tool for cutting-edge biomedical research.

Abstract: Disclosed is a turbine fracturing apparatus. The turbine fracturing apparatus includes: a main power assembly and an auxiliary power assembly. The main power assembly includes a first power source and a piston pump connected to the first power source; the first power source outputs power to the piston pump, and the piston pump outputs a first liquid. The auxiliary power assembly includes a second power source, a load sensitive system connected to the second power source, and an auxiliary power device; the second power source outputs power to the load sensitive system, the load sensitive system is connected to the auxiliary power device and outputs a second liquid for the auxiliary power device. The first liquid is different from the second liquid, and the first liquid and the second liquid have certain pressure. The load sensitive system is configured to regulate a pressure of the second liquid in real time according to pressure of the second liquid required by the auxiliary power device.

Abstract: Disclosed is a turbine fracturing apparatus. The turbine fracturing apparatus includes: a main power assembly and an auxiliary power assembly. The main power assembly includes a first power source and a piston pump connected to the first power source; the first power source outputs power to the piston pump, and the piston pump outputs a first liquid. The auxiliary power assembly includes a second power source, a load sensitive system connected to the second power source, and an auxiliary power device; the second power source outputs power to the load sensitive system, the load sensitive system is connected to the auxiliary power device and outputs a second liquid for the auxiliary power device. The first liquid is different from the second liquid, and the first liquid and the second liquid have certain pressure. The load sensitive system is configured to regulate a pressure of the second liquid in real time according to pressure of the second liquid required by the auxiliary power device.

Abstract: A two-photon stimulated emission depletion composite microscope, comprising a two-photon imaging unit (100) and an STED imaging unit (200), wherein the two-photon imaging unit (100) can be used for a relatively thick sample, and the STED super-resolution imaging unit can be used for a region of interest on a surface of a sample, and the microscope makes light spots generated by an excitation light and a depletion light after being focused by an objective lens (OL) accurately coincide in a three-dimensional distribution. The two-photon stimulated emission depletion composite microscope (10) integrates two functions of STED imaging and two-photon imaging and makes the two types of light spots generated by an excitation light and a depletion light after being focused by an objective lens accurately coincide in a three-dimensional distribution, thereby providing a powerful tool for cutting-edge biomedical research.

Abstract: Disclosed is a turbine fracturing apparatus. The turbine fracturing apparatus includes: a main power assembly and an auxiliary power assembly. The main power assembly includes a first power source and a piston pump connected to the first power source; the first power source outputs power to the piston pump, and the piston pump outputs a first liquid. The auxiliary power assembly includes a second power source, a load sensitive system connected to the second power source, and an auxiliary power device; the second power source outputs power to the load sensitive system, the load sensitive system is connected to the auxiliary power device and outputs a second liquid for the auxiliary power device. The first liquid is different from the second liquid, and the first liquid and the second liquid have certain pressure. The load sensitive system is configured to regulate a pressure of the second liquid in real time according to pressure of the second liquid required by the auxiliary power device.

Abstract: A STED super-resolution microscope capable of quick beam combination is disclosed, which includes a STED imaging unit and a beam combination test unit. The excitation light and the depletion light are accurately combined by the beam combination test unit, so that the imaging light spots of the two light beams passing through the STED imaging unit can accurately coincide with each other, thereby obtaining a better super-resolution imaging effect.

Abstract: A two-photon stimulated emission depletion composite microscope, comprising a two-photon imaging unit (100) and an STED imaging unit (200), wherein the two-photon imaging unit (100) can be used for a relatively thick sample, and the STED super-resolution imaging unit can be used for a region of interest on a surface of a sample, and the microscope makes light spots generated by an excitation light and a depletion light after being focused by an objective lens (OL) accurately coincide in a three-dimensional distribution. The two-photon stimulated emission depletion composite microscope (10) integrates two functions of STED imaging and two-photon imaging and makes the two types of light spots generated by an excitation light and a depletion light after being focused by an objective lens accurately coincide in a three-dimensional distribution, thereby providing a powerful tool for cutting-edge biomedical research.

Abstract: A two-photon stimulated emission depletion composite microscope using continuous light loss, the microscope comprising: a two-photon imaging unit (100) and an STED imaging unit (200), wherein for thicker samples, the two-photon imaging unit (100) can be used, and for regions of interest on the surface of samples, the STED super-resolution imaging unit (200) can be used. The two-photon stimulated emission depletion composite microscope using continuous light loss integrates two functions of STED imaging and two-photon imaging, so as to provide a powerful tool for cutting-edge biomedical research.

Abstract: Disclosed is a STED super-resolution microscope capable of quick beam combination, which includes a STED imaging unit and a beam combination test unit. The excitation light and the depletion light are accurately combined by the beam combination test unit, so that the imaging light spots of the two light beams passing through the STED imaging unit can accurately coincide with each other, thereby obtaining a better super-resolution imaging effect.

Abstract: Disclosed are a low-fluorescence-photobleaching confocal imaging method and system. A confocal image is first selected as a reference image, and a threshold is set based on pixel values of the reference image. Then the density of fluorescent molecules in a pixel is determined based on a result of comparison of a real-time fluorescence intensity feedback and the threshold. Finally, an illumination time for the pixel is controlled based on the density of fluorescent molecules in the pixel to obtain a low-fluorescence-photobleaching confocal imaging image. The low-fluorescence-photobleaching confocal imaging method and system provided herein control the illumination time for each object-side pixel to make more efficient use of fluorescence information and reduce fluorescence photobleaching without sacrificing image quality, and so can be applied to a variety of biological samples. Further provided is a low-fluorescence-photobleaching confocal imaging system.