Abstract: Disclosed is a field effect transistor-based biosensor for detecting whole-cell bacteria which includes a source, a drain, and a biosensing member disposed between the source and the drain. The biosensing member includes at least one semiconductor wire, a surface modification layer, and a plurality of detecting elements. The semiconductor wire serves as a semiconductor channel interconnecting the source and the drain, and has a length so as to permit the biosensing member to capture the whole-cell bacteria. Also disclosed is a field effect transistor-based biosensor assembly including the biosensor.

Abstract: A photodetecting device and method of using the same are provided. Light is used to irradiate the optical filter layer of the photodetecting device and positions of the electrons and the holes in the polycrystalline silicon nano-channel layer are rearranged by the light with a wavelength range capable of passing through the optical filter layer. The current between the source and the drain is changed by rearranging the positions of the electrons and the holes, so as to generate a current difference. The intensity of the light is calculated by the current difference.

Abstract: A photodetecting device and method of using the same are provided. Light is used to irradiate the optical filter layer of the photodetecting device and positions of the electrons and the holes in the polycrystalline silicon nano-channel layer are rearranged by the light with a wavelength range capable of passing through the optical filter layer. The current between the source and the drain is changed by rearranging the positions of the electrons and the holes, so as to generate a current difference. The intensity of the light is calculated by the current difference.

Abstract: A photodetecting device and method of using the same are provided. The photodetecting device includes a transistor, a silicon nano-channel and a filter dye layer. The transistor includes a source, a drain and a gate. The silicon nano-channel connects the source and the drain, and is configured to receive light. The filter dye layer is over a light-receiving surface of the silicon nano-channel.

Abstract: The present invention relates to a method for detecting a target molecule, comprising forming a capturing complex comprising the target molecule; and binding the capturing complex with a signal amplifier, wherein the capturing complex has a net electrical charge; the signal amplifier has affinity to the capturing complex and has a like net electrical charge of the net electrical charge of the capturing complex. The invention improves the detection sensitivity and sensing limit of the detection.

Abstract: The present invention relates to a method for establishing a quantitative landscape of a group of target nucleic acid molecules. The method comprises conducting a plurality of hybridization reactions for quantifying each target nucleic acid molecule of the group of target nucleic acid molecules to generate a plurality of quantitative signals; generating ratios between two quantitative signals; and consolidating the ratios for constructing the quantitative landscape of the group of target nucleic acid molecules. The method according to the invention is able to profile numerous target nucleic acid molecules to provide a big data standardization means for a variety of applications with high sensitivity and wide dynamic range.

Abstract: A photodetecting device and method of using the same are provided. The photodetecting device includes a transistor, a silicon nano-channel and a filter dye layer. The transistor includes a source, a drain and a gate. The silicon nano-channel connects the source and the drain, and is configured to receive light. The filter dye layer is over a light-receiving surface of the silicon nano-channel.

Abstract: The present invention relates to a method for detecting methylation of a target oligonucleotide molecule. The method comprises obtaining an electrical change occurring due to the binding of an electrically charged methylation detecting molecule and the target oligonucleotide molecule; wherein the electrically charged methylation detecting molecule has affinity to a methylated cytosine nucleotide. The invention improves the detection sensitivity and accuracy of the oligonucleotide methylation detection.

Abstract: The present invention relates to a detection unit comprising a solid surface and a partially neutral single-stranded oligonucleotide comprising a first portion. The first portion is attached to the solid surface; the length of the first portion is about 50% of the total length of the partially neutral single-stranded oligonucleotide; and the first portion comprises at least one neutral nucleotide and at least one unmodified nucleotide. The invention improves the detection sensitivity and accuracy of the detection of biomolecules.

Abstract: The present invention relates to a method for detecting a target molecule, comprising forming a capturing complex comprising the target molecule; and binding the capturing complex with a signal amplifier, wherein the capturing complex has a net electrical charge; the signal amplifier has affinity to the capturing complex and has a like net electrical charge of the net electrical charge of the capturing complex. The invention improves the detection sensitivity and sensing limit of the detection.

Abstract: A method of using Neutrilized DNA (N-DNA) as a surface probe for a high throughput detection platform is disclosed. FET and SPRi are used as high throughput detection platforms to demonstrate that the N-DNA surface probe produces good results and enhances detection sensitivity. The N-DNA modifies the charged oxygen ions (O?) on the phosphate backbone through methylation, ethylation, propylation, or alkylation, so that the backbone is not charged after this modification to increase the hybridization efficiency, sensitivity and to make the signal more clear.

Abstract: A cell mobility characteristics sensing apparatus including a laser light source, a light sensor, an analyzing chip, and a display is disclosed. The laser light source emits laser beams to a cell sample. The light sensor senses scattered laser beams formed by the cell sample scattering the laser beams at a plurality of time points to obtain a plurality of laser scattering patterns corresponding to the plurality of time points respectively. The analyzing chip obtains a laser scattering pattern fluctuations information of the plurality of laser scattering patterns varied with the plurality of time points to estimate the mobility characteristics of the cells in the cell sample. The display shows the mobility characteristics of the cells in the cell sample estimated by the analyzing chip.

Abstract: A method of manufacturing a nanoparticle chain is disclosed. The method comprises the steps of: providing a single-stranded circular primer with a determined length, and amplifying the single-stranded circular primer into single-stranded DNA nanotemplate by an isothermal nucleotide amplification reaction such that an end of the single-stranded DNA nanotemplate is fixed to a surface of a substrate; and adding a single-stranded DNA probe conjugated with nanoparticle at one end of which, and attaching the single-stranded DNA probe to the corresponding sequence on the single-stranded DNA nanotemplate to form a nanoparticles chain. The method of manufacturing a nanoparticle chain further comprises providing a fluid, and the flowing direction of the fluid controls the aligning direction of the nanoparticle chain. Wherein, the inter-nanoparticle distance of the nanoparticle chain can be adjusted by adjusting a reaction temperature or adding the single-stranded DNA probe without conjugating with nanoparticles.

Abstract: A system of a wireless physiological signal integration is provided. The system includes a wireless transmission sensor chip and a drug delivering system, wherein the wireless transmission sensor chip includes a sensor sensing a physiological signal of a patient, a signal conversion module converting the physiological signal into a converted signal, and a wireless transmission module wirelessly transmitting the converted signal, and the drug delivering system determines a dose of a drug and a timing for providing the drug according to the converted signal.

Abstract: A microfluidic control apparatus operating method is disclosed. The microfluidic control apparatus operating method is applied in a microfluidic control apparatus, and the microfluidic control apparatus includes a photoconductive material layer and a flow passage. The microfluidic control apparatus operating method includes steps of (a) when a light with a specific optical pattern is emitted toward the photoconductive material layer, at least three virtual electrodes being formed on the photoconductive material layer according to the specific optical pattern; (b) when the specific optical pattern changes, the at least three virtual electrodes also changing to generate an electro-osmotic force to control a moving state of a microfluid in the flow passage.

Abstract: A method that modifies surface properties of a substrate by manipulating the immobilized biomolecules in mild biological condition. The manipulation comprised steps of: providing a biomolecule combined with at least one ssDNA combined with a first protein through an affinity binding tag; adding a second ssDNA conjugated with a second protein with a concentration greater than that of the first protein; and replacing the first protein on the ssDNA with the second protein through chemical competitive principle. The invention may comprise the steps with proper design of biotinylated DNA probes, the functionalized ssDNA nanotemplates can be recovered to its unbound state through a thermodynamic principle.

Abstract: A method of manufacturing a nanoparticle chain is disclosed. The method comprises the steps of: providing a single-stranded circular primer with a determined length, and amplifying the single-stranded circular primer into single-stranded DNA nanotemplate by an isothermal nucleotide amplification reaction such that an end of the single-stranded DNA nanotemplate is fixed to a surface of a substrate; and adding a single-stranded DNA probe conjugated with nanoparticle at one end of which, and attaching the single-stranded DNA probe to the corresponding sequence on the single-stranded DNA nanotemplate to form a nanoparticles chain. The method of manufacturing a nanoparticle chain further comprises providing a fluid, and the flowing direction of the fluid controls the aligning direction of the nanoparticle chain. Wherein, the inter-nanoparticle distance of the nanoparticle chain can be adjusted by adjusting a reaction temperature or adding the single-stranded DNA probe without conjugating with nanoparticles.

Abstract: A method that modifies surface properties of a substrate by manipulating the immobilized biomolecules in mild biological condition. The manipulation comprised steps of: providing a biomolecule combined with at least one ssDNA combined with a first protein through an affinity binding tag; adding a second ssDNA conjugated with a second protein with a concentration greater than that of the first protein; and replacing the first protein on the ssDNA with the second protein through chemical competitive principle. The invention may comprise the steps with proper design of biotinylated DNA probes, the functionalized ssDNA nanotemplates can be recovered to its unbound state through a thermodynamic principle.

Abstract: A microfluidic control apparatus and operating method thereof. The microfluidic control apparatus includes a photoconductive material layer and a flow passage. When a light with a specific optical pattern is emitted toward the photoconductive material layer, at least three virtual electrodes are formed on the photoconductive material layer according to the specific optical pattern. The at least three virtual electrodes include a first virtual electrode, a second virtual electrode and a third virtual electrode disposed beside the first virtual electrode. There is a specific proportion among a distance between first virtual electrode and third virtual electrode, a width of first virtual electrode, a distance between first virtual electrode and second virtual electrode, and a width of second virtual electrode. When the specific optical pattern changes, the at least three virtual electrodes also change to generate an electro-osmotic force to control the moving state of a microfluid in a flow passage.

Abstract: A continuous testing method for testing the concentration of a target object in a fluid is provided. The method comprises the following steps. A focused light is provided in the fluid to separate the target object from a non-target object in the fluid by changing the movement direction of the target object and the non-target object. The fluid having separated out the non-target object is enabled to react with a reagent. A signal is provided to pass through the fluid having reacted with the reagent. The signal passing through the fluid is received and an electronic signal is outputted corresponding to the input signal. The concentration of the target object is acquired according to the electronic signal.