Patents by Inventor Yuh-Teh Li

Yuh-Teh Li has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • KDN-cleaving sialidase isolated from hepatopancreas of mollusks
    Patent number: 5449615
    Abstract: A sialidase capable of cleaving a glycoconjugate to yield 3-deoxy-D-glycero-D-galacto-2-nonulosonic acid (KDN) is isolated from hepatopancreas of mollusks. Preferably, the sialidase is isolated by processing crude oyster hepatopancreas to form a crude extract of the sialidase enzyme. The crude enzyme is further concentrated and refined utilizing sequential column chromatography fractogel EMD DEAE-650 (M), sephacryl S-200, and fractogel EMD SP 650 (M) chromatography, respectively. Using this procedure, the sialidase is purified over 155 fold with a 17% recovery. Ferric ion exerts a three fold stimulation in the activity of the sialidase, at a concentration of 3 mM. The sialidase cleaves 4-methylumbelliferyl-KDN, KDN.alpha.2.fwdarw.3Gal.beta.1.fwdarw.4GlcCer, KDN.alpha.2.fwdarw.6Gal.beta.1.fwdarw.4GlcCer, or KDN.alpha.2.fwdarw.6GalNAc-o1 to yield KDN.
    Type: Grant
    Filed: September 26, 1994
    Date of Patent: September 12, 1995
    Inventors: Yuh-Teh Li, Su-Chen Li