Abstract: The present disclosure disclosed recombinase aided amplification (RAA) primers and kits for the detection of hepatitis c virus. Nucleotide sequences of the RAA primers include: an upstream primer: 5?-FITC-CTTGGGATATGATGATGAACTGGTCACCTAC-3? (SEQ ID NO. 1); and a downstream primer: 5?-Biotin-AAGAGTAGCATCACAATCAGAACCTTAGCC-3? (SEQ ID NO. 2). The HCV detection by using the RAA primers screened by the present disclosure has good specificity and high sensitivity (at least 10 copies/?L can be detected). The RAA primers can be used to prepare an HCV detection kit and construct an RAA amplification system. And combined with a lateral flow chromatography technology, the present disclosure can achieve rapid and low-cost detection of HCV and visual result judgment, no complex professional background is required, the use process is convenient and fast, and the detection results are safe and reliable.

Abstract: The present invention belongs to the bioengineering field, and relates to a method for fermentation production of L-theanine by using an Escherichia coli genetically engineered bacterium. The engineered bacterium is obtained by serving a strain as an original strain, wherein the strain is obtained after performing a single copy of T7RNAP, a dual copy of gmas, xylR knockout, and sucCD knockout on an Escherichia coli W3110 genome, and by integrating genes xfp, pta, acs, gltA, and ppc, and knocking out ackA on the genome. The present invention has a high yield, and stable production performance; after 20-25 h, L-theanine has a titer of 75-80 g/L, and the yield is up to 52-55%. The fermentation broth is purified by membrane separation in combination with a cation-anion resin series technique. Moreover, the one-step crystallization yield is 72.3% and the L-theanine final product has a purity of 99%.

Abstract: The disclosure discloses a genetically engineered strain for sarcosine production as well as a construction method and application. The genetically engineered strain is obtained by using Escherichia coli as a host and by integrating a single copy of imine reductase gene dpkA on its genome; singly copying citrate synthase gene gltA; knocking out glyoxylate cycle inhibitor gene iclR; knocking out malate synthase gene aceB; integrating a single copy of isocitrate lyase gene aceA; integrating a single copy of membrane-bound transhydrogenase gene pntAB; knocking out 2-ketate reductase gene ycdW; integrating a single copy of phosphoenolpyruvate carboxylase gene ppc; and knocking out pyruvate kinase gene pykF. After system metabolism transformation, the engineered strain can synthesize sarcosine with glucose and methylamine as main raw materials. The sarcosine titer can reach 10 g/L after fermentation for 30 h in a 5 L fermenter.

Abstract: The present invention belongs to the bioengineering field, and relates to a method for fermentation production of L-theanine by using an Escherichia coli genetically engineered bacterium. The engineered bacterium is obtained by serving a strain as an original strain, wherein the strain is obtained after performing a single copy of T7RNAP, a dual copy of gmas, xylR knockout, and sucCD knockout on an Escherichia coli W3110 genome, and by integrating genes xfp, pta, acs, gltA, and ppc, and knocking out ackA on the genome. The present invention has a high yield, and stable production performance; after 20-25 h, L-theanine has a titer of 75-80 g/L, and the yield is up to 52-55%. The fermentation broth is purified by membrane separation in combination with a cation-anion resin series technique. Moreover, the one-step crystallization yield is 72.3% and the L-theanine final product has a purity of 99%.

Abstract: The present disclosure provides a method for monitoring a network status of a Zigbee node. The method includes: acquiring a value of a status tag configured on the Zigbee node, the value of the status tag indicating a current network status of the Zigbee node; and determining that the value of the status tag equals a first preset value and a preset condition associated with the status tag is met. The first preset value indicates that the Zigbee node has joined a Zigbee coordinator but has not joined a gateway. The method also includes: in response to determining that the value of the status tag equals the first preset value and the preset condition is met, restarting the Zigbee node and resetting the value of the status tag to an initial value. The initial value indicates that the Zigbee node has not joined the Zigbee coordinator.

Abstract: The disclosure discloses a genetically engineered strain for sarcosine production as well as a construction method and application. The genetically engineered strain is obtained by using Escherichia coli as a host and by integrating a single copy of imine reductase gene dpkA on its genome; singly copying citrate synthase gene gltA; knocking out glyoxylate cycle inhibitor gene iclR; knocking out malate synthase gene aceB; integrating a single copy of isocitrate lyase gene aceA; integrating a single copy of membrane-bound transhydrogenase gene pntAB; knocking out 2-ketate reductase gene ycdW; integrating a single copy of phosphoenolpyruvate carboxylase gene ppc; and knocking out pyruvate kinase gene pykF. After system metabolism transformation, the engineered strain can synthesize sarcosine with glucose and methylamine as main raw materials. The sarcosine titer can reach 10 g/L after fermentation for 30 h in a 5 L fermenter.

Abstract: A tracked vehicle is provided. The tracked vehicle includes a frame, a driving mechanism installed in the frame, and two track wheels respectively arranged on the left and right sides of the frame. The driving mechanism drives the track wheels to rotate. The track wheel includes a track body, a first main support, a second main support and a third main support provided in the track body. The first main support, the second main support and the third main support are connected to the vehicle frame through the first, second and third connecting rods respectively. The second main support is provided with an auxiliary support frame having one end hinged to the second main support, and the other end of the auxiliary support frame is provided with a first roller contacting with the inner side of the track body.

Abstract: A tracked vehicle is provided. The tracked vehicle includes a frame, a driving mechanism installed in the frame, and two track wheels respectively arranged on the left and right sides of the frame. The driving mechanism drives the track wheels to rotate. The track wheel includes a track body, a first main support, a second main support and a third main support provided in the track body. The first main support, the second main support and the third main support are connected to the vehicle frame through the first, second and third connecting rods respectively. The second main support is provided with an auxiliary support frame having one end hinged to the second main support, and the other end of the auxiliary support frame is provided with a first roller contacting with the inner side of the track body.

Abstract: The present disclosure provides a method for monitoring a network status of a Zigbee node. The method includes: acquiring a value of a status tag configured on the Zigbee node, the value of the status tag indicating a current network status of the Zigbee node; and determining that the value of the status tag equals a first preset value and a preset condition associated with the status tag is met. The first preset value indicates that the Zigbee node has joined a Zigbee coordinator but has not joined a gateway. The method also includes: in response to determining that the value of the status tag equals the first preset value and the preset condition is met, restarting the Zigbee node and resetting the value of the status tag to an initial value. The initial value indicates that the Zigbee node has not joined the Zigbee coordinator.

Abstract: Disclosed are anti-miR compositions and methods of use of the same for treatment of or reducing the occurrence of thrombosis and thrombosis related diseases and disorders by reducing platelet activation.

Abstract: Disclosed are anti-miR compositions and methods of use of the same for treatment of or reducing the occurrence of thrombosis and thrombosis related diseases and disorders by reducing platelet activation.

Abstract: A method and a system for information push are described, the method including: receiving a business search command determined by an operation of a user; responding the business search command, and sending a business search request which comprises current location information of the user to a first server to query a business matching the current location information of the user; and receiving information of the business in response to the business search request returned by the first server. Product information can be accurately pushed to a user. In addition, a real-time update for the product information can be made by a business, and the push effect of the product information can be improved.