Abstract: The present invention provides methods of producing single-stranded DNA (ssDNA) from a double-stranded DNA (dsDNA) template. The method includes selecting either the (+)-strand or the (?)-strand of a dsDNA template as a target ssDNA, wherein if the target ssDNA is the (+)-strand of the dsDNA template, a biotin label is added onto the reverse primer, and wherein if the target ssDNA is the (?)-strand of the dsDNA template, a biotin label is added onto the forward primer. Next PCR is performed to produce biotinylated dsDNA fragments. Once the PCR is terminated, the biotinylated dsDNA fragments are purified. Next the target ssDNA is separated from the biotinylated dsDNA by immobilizing the biotinylated dsDNA onto a surface and precipitating the target ssDNA.

Abstract: The invention relates to methods of treating diseases comprising administering to a subject a composition comprising the recombinant adeno-associated virus (rAAV). The rAAV is produced by a method comprising: obtaining a template DNA sequence containing a [ITR-cargo-ITR] DNA sequence motif; designing a PCR primer pair such that the 3? terminus of both the forward and reverse PCR primers overlap only about the last 2-8 bases of the A/A? ITR sequences and the 5? terminus of both the forward and reverse PCR primers extend into about 20-35 bases of the flanking sequences; performing PCR with cycling parameters comprising a combined annealing/extension step at a temperature greater than 70° C., thereby producing a plurality of amplicon polynucleotides containing the desired [ITR-cargo-ITR] DNA sequence motif; transfecting the amplicon polynucleotides containing the desired [ITR-cargo-ITR] DNA sequence motif into a packaging cell line; and purifying the lysed cells to collect a quantity of rAAV.

Abstract: The present invention relates generally to PCR-based in vitro diagnostics and methods of treatment for SARS-CoV-2, the virus that causes COVID-19, and more specifically, to compositions and methods for the detection of wildtype and variants of SARS-CoV-2 in a patient specimen and methods of treating SARS-CoV-2 based on said detection.

Abstract: A composition comprising micron or submicron particles covered by a monolayer of nucleic acid wherein the nucleic acid may be recovered from the submicron particles is claimed. Methods of attaching a nucleic acid to an object for authentication and methods of authenticating an object are also claimed.

Abstract: Methods of incorporating coded DNA into elastomeric materials and compositions thereof are claimed. Methods of recovering information from elastomeric materials with coded DNA and authenticating silicone objects with coded DNA are also claimed.

Abstract: The present invention relates to systems and methods to produce recombinant adeno-associated virus (rAAV) utilizing one or more DNA constructs manufactured via polymerase chain reaction (PCR).

Abstract: A composition comprising submicron particles covered by a monolayer of nucleic acid wherein the nucleic acid may be recovered from the submicron particles is claimed. Methods of attaching a nucleic acid to an object for authentication and methods of authenticating an object are also claimed.

Abstract: A composition comprising submicron particles covered by a monolayer of nucleic acid wherein the nucleic acid may be recovered from the submicron particles is claimed. Methods of attaching a nucleic acid to an object for authentication and methods of authenticating an object are also claimed.

Abstract: Methods of incorporating coded DNA into elastomeric materials and compositions thereof are claimed. Methods of recovering information from elastomeric materials with coded DNA and authenticating silicone objects with coded DNA are also claimed.

Abstract: The present disclosure relates to a cosmetic active ingredient including an extract of Fucus vesiculosus. The present disclosure further relates to a topical composition including the cosmetic active ingredient The present disclosure further relates to a method of producing the cosmetic active ingredient. The method includes adding a coarse ground biomass of Fucus vesiculosus to at least one of water or a polyethylene glycol (PEG) solution for a suitable period of time.

Abstract: The present invention relates to a cosmetic active ingredient for improving the appearance of the skin beneath the eyes. More particularly, the present invention relates to a cosmetic active ingredient comprising an extract of the marine algae, Fucus vesiculosus. The present invention also relates to topical cosmetic compositions comprising as an active component an extract of the marine algae, Fucus vesiculosus, alone or in combination with additional cosmetic active ingredients, to reduce the appearance of dark circles in skin beneath the eye.

Abstract: The present invention relates to a cosmetic and/or pharmaceutical active component for stimulating proteasome activity of skin cells comprising the broth obtained by inoculating a biomass of the algae, Gracilaria spp., particularly the algae, Gracilaria tikvahiae, with a yeast culture, and allowing fermentation of the biomass to produce a fermentation broth. Also described is a cosmetic and/or pharmaceutical active component for stimulating proteasome activity of skin cells comprising an extract of the algae, Gracilaria tikvahiae. The present invention also relates to topical compositions comprising as a cosmetic and/or pharmaceutical active component a ferment of the algae, Gracilaria spp., particularly the algae, Gracilaria tikvahiae, or an extract of the algae, Gracilaria tikvahiae, or a combination of both.

Abstract: Exemplary embodiments of the present invention provide a method for assessing a proportion of one or more cotton species in an article comprising cotton. The method includes providing a sample including cotton fibers from the article comprising cotton. Cotton DNA is extracted from the cotton fibers to provide extracted cotton DNA. The extracted cotton DNA is analyzed to identify a presence of one or more cotton species included in the article comprising cotton. Proportions of the one or more cotton species included in the article comprising cotton are assessed.

Abstract: The present invention relates to a cosmetic active ingredient for improving the appearance of the skin beneath the eyes. More particularly, the present invention relates to a cosmetic active ingredient comprising an extract of the marine algae, Fucus vesiculosus. The present invention also relates to topical cosmetic compositions comprising as an active component an extract of the marine algae, Fucus vesiculosus, alone or in combination with additional cosmetic active ingredients, to reduce the appearance of dark circles in skin beneath the eye.

Abstract: The present invention relates to a cosmetic and/or pharmaceutical active component for stimulating proteasome activity of skin cells comprising the broth obtained by inoculating a biomass of the algae, Gracilaria spp., particularly the algae, Gracilaria tikvahiae, with a yeast culture, and allowing fermentation of the biomass to produce a fermentation broth. Also described is a cosmetic and/or pharmaceutical active component for stimulating proteasome activity of skin cells comprising an extract of the algae, Gracilaria tikvahiae. The present invention also relates to topical compositions comprising as a a cosmetic and/or pharmaceutical active component a ferment of the algae, Gracilaria spp., particularly the algae, Gracilaria tikvahiae, or an extract of the algae, Gracilaria tikvahiae, or a combination of both.

Abstract: Isolated nucleic acid sequences and polypeptides encoded thereby for epothilone B hydroxylase and mutants and variants thereof and a ferredoxin located downstream from the epothilone B hydroxylase gene are provided. Also provided are vectors and cells containing these vectors. In addition, methods for producing recombinant microorganisms, methods for using these recombinant microorganism to produce hydroxyalkyl-bearing epothilones and an epothilone analog produced by a mutant of epothilone B hydroxylase are provided.

Abstract: Isolated nucleic acid sequences and polypeptides encoded thereby for epothilone B hydroxylase and mutants and variants thereof and a ferredoxin located downstream from the epothilone B hydroxylase gene are provided. Also provided are vectors and cells containing these vectors. In addition, methods for producing recombinant microorganisms, methods for using these recombinant microorganism to produce hydroxyalkyl-bearing epothilones and an epothilone analog produced by a mutant of epothilone B hydroxylase are provided.

Abstract: Isolated nucleic acid sequences and polypeptides encoded thereby for epothilone B hydroxylase and mutants and variants thereof and a ferredoxin located downstream from the epothilone B hydroxylase gene are provided. Also provided are vectors and cells containing these vectors. In addition, methods for producing recombinant microorganisms, methods for using these recombinant microorganism to produce hydroxyalkyl-bearing epothilones and an epothilone analog produced by a mutant of epothilone B hydroxylase are provided.

Abstract: Isolated nucleic acid sequences and polypeptides encoded thereby for epothilone B hydroxylase and mutants and variants thereof and a ferredoxin located downstream from the epothilone B hydroxylase gene are provided. Also provided are vectors and cells containing these vectors. In addition, methods for producing recombinant microorganisms, methods for using these recombinant microorganism to produce hydroxyalkyl-bearing epothilones and an epothilone analog produced by a mutant of epothilone B hydroxylase are provided.