Abstract: Provided are a method for detecting a gene mutation using an FFPE tissue section containing tumor cells regardless of the percentage of tumor cells, the method being capable of increasing the number of detectable gene mutations and mutant allele frequency, and a method capable of differentiating, even in the absence of blood samples, a somatic cell mutation from a germ cell line mutation. A method for detecting a gene mutation according to the present invention comprises: a dissociation step for dissociating a single cell population from a formalin-fixed paraffin-embedded tissue section containing tumor cells; a separation step for obtaining a tumor fraction containing the tumor cells from the single cell population; a collection step for collecting a nucleic acid molecule from the tumor fraction; and a sequencing step for subjecting the nucleic acid molecule to sequencing.

Abstract: An ophthalmologic imager comprises an illuminating optical system for projecting an illuminating light beam onto an ocular fundus, a light-receiving optical system having a focusing lens and serving to guiding the light reflected from the ocular fundus to an imaging section, and a focusing target projecting optical system having a bar mirror detachably inserted in the optical path of the illuminating optical system and serving to project split focusing target light beams onto the ocular fundus through the bar mirror and the illuminating optical system. A mirror image of the bar mirror is projected onto the ocular fundus by using the illuminating light beam. The ophthalmologic imager further comprises a judging device which judges the sharpness of the contour of the mirror image or the focusing target light imagers from the output of the light-receiving section and judges the focusing state by the focusing lens from the sharpness.

Abstract: An ophthalmologic imager comprises an illuminating optical system (10) for projecting an illuminating light beam onto an ocular fundus, a light-receiving optical system (an imaging optical system (20), an observing optical system (30)) having a focusing lens and serving to guiding the light reflected from the ocular fundus to an imaging section (CCD6b), and a focusing target projecting optical system (190) having a bar mirror (190a) detachably inserted in the optical path of the illuminating optical system and serving to project split focusing target light beams onto the ocular fundus through the bar mirror (190a) and the illuminating optical system (10). A mirror image (190a?) of the bar mirror is projected onto the ocular fundus by using the illuminating light beam.

Abstract: An ophthalmologic photographing apparatus including: an apparatus main body having an illumination system for illuminating an eye to be examined and an observation system for observing an image of the eye to be examined which is illuminated by the illumination system; a photographing device for taking a light flux from the eye to be examined from an optical path of the observation system, photographing the image of the eye to be examined and recording the image; a storage unit for storing a photographing condition related to the apparatus main body and a photographing processing condition related to the photographing device for each of plural kinds of photographing processing modes in photographing and storing the eye to be examined; a designating unit for designating a photographing processing mode for the eye to be examined; and a setting unit for reading out a photographing condition related to the apparatus main body and a photographing processing condition related to the photographing device for a corres

Abstract: An ophthalmic apparatus including a photographic optical system (31, 48) capable of photographing a tested eye (E) and obtaining an image of the tested eye, a drive device (76, 83, 90) configured to drive the photographic optical system relative to the tested eye in three-dimensions, an alignment target projecting optical system (56) to project alignment light flux to the tested eye, and a control device (84) configured to control the drive device based on a ate of reflection light flux formed by reflection of the alignment light flux projected by the alignment target projecting optical system on the tested eye, when a flare occurs in the reflection light flux of the alignment light flux reflected on the tested eye, the control device being configured to control the drive device to move the photographic optical system in a direction where the flare decreases.

Abstract: There is provided an ophthalmologic photographing apparatus in which light from an observation light source or light from a photographing light source is irradiated to an eye to be examined, reflected light from the eye to be examined by the irradiation is guided to a photographing unit having a color area CCD as an image pickup device through an observation optical system in an ophthalmologic photographing apparatus main body, an image of the eye to be examined is imaged onto the color area CCD to photograph the image of the eye to be examined, and the image of the eye to be examined is displayed on an image display device in accordance with an image pickup signal from the image pickup device.

Abstract: There is provided an ophthalmologic photographing apparatus including: an apparatus main body having an illumination system for illuminating an eye to be examined and an observation system for observing an image of the eye to be examined which is illuminated by the illumination system; a photographing device for taking a light flux from the eye to be examined from an optical path of the observation system, photographing the image of the eye to be examined and recording the image; a storage unit for storing a photographing condition related to the apparatus main body and a photographing processing condition related to the photographing device for each of plural kinds of photographing processing modes in photographing and storing the eye to be examined; a designating unit for designating a photographing processing mode to the eye to be examined; and a setting unit for reading out a photographing condition related to the apparatus main body and a photographing processing condition related to the photographing de

Abstract: In an operation microscope, when an observation velocity of an observation field of a main microscope body is set to a desired velocity and then an observation magnification of the observation field of the main microscope body is changed and the focal point thereof is moved, each encoder detects changing of the observation magnification and moving of the focal point and then outputs a detection result to an arithmetic-logic unit. The arithmetic-logic unit calculates the drive condition that a moving velocity of the observation field of the main microscope body after the observation magnification is changed and the focal point is moved is a desired velocity, based on the detection result of each encoder. By using a Y-axis actuator, an X-axis actuator, a vertical direction moving unit and a horizontal direction moving unit, the main microscope body is moved under the control of a CPU in accordance with the drive condition obtained by the arithmetic-logic unit.