Abstract: The present invention provides a novel target analysis chip and analysis method for directly detecting a target such as a microRNA without performing PCR.

Abstract: A target analysis method and target analyzing chip are provided to directly detect a target, such as microRNA, without performing PCR. The target analysis method of the present embodiment includes: a process in which a target in a sample, a labeled probe that binds to the target, and a carrier-binding probe that binds to the target and to a carrier, contact each other, and the target, the labeled probe, and the carrier-binding probe react to bind to each other to form a first bound body; a process in which the first bound body and the carrier contact each other, and the first bound body and the carrier react to bind to each other to form a second bound body; a process to concentrate the carrier; and a process in which the target in the sample is analyzed by detecting a label of the labeled probe bound to the concentrated carrier.

Abstract: The present invention provides a novel target analysis chip and analysis method for directly detecting a target such as a microRNA without performing PCR.

Abstract: A cell culture device comprising: a channel having an inlet end for the introduction of a liquid and a bottom surface, wherein said channel is configured for the flow through of said liquid; and a plurality of recesses for containing cells, which are formed on said bottom surface of said channel; wherein said plurality of recesses are closely arranged on said bottom surface of said channel.

Abstract: To provide a cell culture device, a cell culture system and a cell culture method capable of suppressing fluctuations in pressure in a culture fluid, and suppressing gas bubbles from flowing into a cell culture section. A cell culture device including: a cell culture section that cultures cells; a storage section that stores a culture fluid; flow paths that connect the cell culture section and the storage section; a fluid delivery device that is provided at the flow paths and that delivers the culture fluid from the storage section to the cell culture section; a pressure equalizing unit that is provided at the flow paths and that suppresses fluctuations in pressure imparted to the culture fluid delivered to the cell culture section; and a pressurization unit that is provided at the flow path at a flow outlet side of the cell culture section and that applies a specific pressure to the culture fluid.

Abstract: An external handle device for a vehicle door is provided in which an operating handle comprising a handle main body that has formed therein a housing recess having a substantially U-shaped cross section and a handle cover that is secured to the handle main body so as to cover the housing recess is disposed so as to be operable on an outer face side of the vehicle door, and an electronic component unit formed by covering at least an electronic component with a jacket member is housed within the housing recess, wherein an extending portion is provided integrally with the jacket member, which is formed from a resilient material, the extending portion being sandwiched between abutting parts of the handle main body and the handle cover. Such configuration prevents rubbing occurring between abutting parts of the handle main body and the handle cover while eliminating necessity for an adhesive.

Abstract: A cell culture method is disclosed which includes: a seeding process of singly seeding single cells on a coating layer of a microchannel, the coating layer being present on an inner wall of the microchannel, and the coating layer containing laminin; and a circulation process of circulating a culture fluid in the microchannel where the single cells have been singly seeded in the seeding process.

Abstract: A fluid agitation method is provided, whereby a swirling flow is generated in a trace amount of fluid, thereby agitating the fluid. The fluid agitation method includes introducing the fluid into an agitation chamber (3) including a wall having an uneven mass distribution, and applying oscillation (F) to the wall with frequencies varying in a predetermined frequency range. The uneven mass distribution of the wall is attained, for example, by arranging a plurality of thickened portions (11 to 18) of different thicknesses in a ring.

Abstract: To provide a cell culture device, a cell culture system and a cell culture method capable of suppressing fluctuations in pressure in a culture fluid, and suppressing gas bubbles from flowing into a cell culture section. A cell culture device including: a cell culture section that cultures cells; a storage section that stores a culture fluid; flow paths that connect the cell culture section and the storage section; a fluid delivery device that is provided at the flow paths and that delivers the culture fluid from the storage section to the cell culture section; a pressure equalizing unit that is provided at the flow paths and that suppresses fluctuations in pressure imparted to the culture fluid delivered to the cell culture section; and a pressurization unit that is provided at the flow path at a flow outlet side of the cell culture section and that applies a specific pressure to the culture fluid.

Abstract: The one aspect of the present invention aims to provide a novel cloning method for human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), which method is based on culture of dispersed single cells without addition of an apoptotic agent. More specifically, the one aspect of the present invention aims to provide a cloning method for hESCs and hiPSCs based on culture of dispersed single cells utilizing shear stress. The above problem is solved by providing a method for culturing dispersed single pluripotent cells, wherein the dispersed single cells are cultured under laminar flow conditions.

Abstract: An external handle device for a vehicle door is provided in which an operating handle comprising a handle main body that has formed therein a housing recess having a substantially U-shaped cross section and a handle cover that is secured to the handle main body so as to cover the housing recess is disposed so as to be operable on an outer face side of the vehicle door, and an electronic component unit formed by covering at least an electronic component with a jacket member is housed within the housing recess, wherein an extending portion is provided integrally with the jacket member, which is formed from a resilient material, the extending portion being sandwiched between abutting parts of the handle main body and the handle cover. Such configuration prevents rubbing occurring between abutting parts of the handle main body and the handle cover while eliminating necessity for an adhesive.

Abstract: This invention relates to technology for analyzing a specific component in a sample liquid, and provides an analyzing tool and an analyzing apparatus. Analyzing tool (Y) includes a liquid inlet (61) at a central portion of the tool and a plurality of channels (51) which communicate with liquid inlet (61) and move the sample liquid introduced through liquid inlet (61) by capillary action from the central portion towards a peripheral portion of the tool. Each channel (51) extends linearly for example from the central portion towards the peripheral portion, and the plurality of channels (51) are arranged radially.

Abstract: A liquid feeding method for feeding liquid in a minute flow path is provided. The minute flow path includes flow paths 43a, 43c, 43d connected to each other directly at respective first ends. The liquid feeding direction of blood S in the minute flow path is controlled by creating a high-pressure state at a second end of the flow path 43a located across the blood S, while creating a low-pressure state or a closed state at a second end of the flow paths 43d, 43c.

Abstract: A fluid agitation method is provided, whereby a swirling flow is generated in a trace amount of fluid, thereby agitating the fluid. The fluid agitation method includes introducing the fluid into an agitation chamber (3) including a wall having an uneven mass distribution, and applying oscillation (F) to the wall with frequencies varying in a predetermined frequency range. The uneven mass distribution of the wall is attained, for example, by arranging a plurality of thickened portions (11 to 18) of different thicknesses in a ring.

Abstract: An analyzing tool (Y) has a liquid-introducing opening (61), one or more flow passages (51) through which a sample liquid introduced from the liquid-introducing opening (61) is moved, and a separation film (8) for filtrating the sample liquid supplied to the liquid-introducing opening (61) and then introducing the liquid filtrated to the one or more flow passages (51). The analyzing tool (Y) is structured such that a liquid sample is filtrated by being advanced in the thickness direction of the separation film (8). The flow passage (51) is structured such that the sample liquid is moved by, for example, a capillary phenomenon.

Abstract: The invention relates to a method of producing an analytical tool having recesses (20) formed therein for moving a sample liquid, a base plate (2) made of polymeric material, and a cover laminated to the base plate (2) to cover the recesses (20). This producing method includes the step of applying a hydrophilic treatment to the inner surfaces of the recesses (20). The hydrophilic treatment includes a primary modifying operation for primarily modifying the properties of the inner surfaces of the recesses (20) by contacting a modifying gas with the inner surfaces of the recesses (20), and a secondary modifying operation for secondarily modifying the properties of the inner surfaces of the recesses (20).

Abstract: A liquid feeding method for feeding liquid in a minute flow path is provided. The minute flow path includes flow paths 43a, 43c, 43d connected to each other directly at respective first ends. The liquid feeding direction of blood S in the minute flow path is controlled by creating a high-pressure state at a second end of the flow path 43a located across the blood S, while creating a low-pressure state or a closed state at a second end of the flow paths 43d, 43c.

Abstract: A cartridge A to be mounted to a separate apparatus is provided. The cartridge includes a liquid introduction port 3 for introducing a sample liquid, and a diluter 4 including a diluent tank 41 for storing a diluent 40 for diluting the sample liquid, a sample liquid measurer 43 for separating a predetermined amount of sample liquid from the sample liquid introduced from the liquid introduction port 3, and a dilution tank 42A for mixing at least part of the sample liquid and at least part of the diluent. The sample liquid measurer 4 includes an introduction flow path 43a extending from the liquid introduction port 3, and a measurement flow path 43c and, an overflow path 43d which are connected to the introduction flow path 43a via a branch portion 43b. The measurement flow path 43c extends toward the dilution tank 42A.

Abstract: A blood testing tool is provided, which can recover blood plasma or blood serum with a high yield without causing hemolysis of blood cells. A recovery porous membrane 1 to be impregnated with blood serum or blood plasma contained in blood and retain it and a separation porous membrane 2 for separating blood cells from the blood are provided. The separation porous membrane 2 is laminated on the recovery porous membrane 1 while satisfying the condition that pores on an upper surface side of the recovery porous membrane have an average pore size that is in a range from 10 to 1000 ?m and is larger than that of pores on a lower surface side of the separation porous membrane, thus providing a blood testing tool according to the present invention.

Abstract: The invention relates to a method of producing an analytical tool having recesses (20) formed therein for moving a sample liquid, a base plate (2) made of polymeric material, and a cover laminated to the base plate (2) to cover the recesses (20). This producing method includes the step of applying a hydrophilic treatment to the inner surfaces of the recesses (20). The hydrophilic treatment includes a primary modifying operation for primarily modifying the properties of the inner surfaces of the recesses (20) by contacting a modifying gas with the inner surfaces of the recesses (20), and a secondary modifying operation for secondarily modifying the properties of the inner surfaces of the recesses (20).