Patents by Inventor Yuiko Miura
Yuiko Miura has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10988741Abstract: A novel luciferase that is distinct from conventional luciferase has been desired. A luciferase mutant comprising an amino acid sequence in which glutamic acid at position 4, arginine at position 11, leucine at position 18 and valine at position 27 are substituted with other amino acids, in the amino acid sequence of SEQ ID NO: 2.Type: GrantFiled: May 6, 2019Date of Patent: April 27, 2021Assignee: JNC CORPORATIONInventors: Satoshi Inouye, Yuiko Miura, Junichi Sato
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Publication number: 20190256826Abstract: A novel luciferase that is distinct from conventional luciferase has been desired. A luciferase mutant comprising an amino acid sequence in which glutamic acid at position 4, arginine at position 11, leucine at position 18 and valine at position 27 are substituted with other amino acids, in the amino acid sequence of SEQ ID NO: 2.Type: ApplicationFiled: May 6, 2019Publication date: August 22, 2019Applicant: JNC CORPORATIONInventors: Satoshi INOUYE, Yuiko MIURA, Junichi SATO
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Patent number: 10377995Abstract: A novel luciferase that is distinct from conventional luciferase has been desired. A luciferase mutant comprising an amino acid sequence in which glutamic acid at position 4, arginine at position 11, leucine at position 18 and valine at position 27 are substituted with other amino acids, in the amino acid sequence of SEQ ID NO: 2.Type: GrantFiled: December 7, 2017Date of Patent: August 13, 2019Assignee: JNC CORPORATIONInventors: Satoshi Inouye, Yuiko Miura, Junichi Sato
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Publication number: 20180119112Abstract: A novel luciferase that is distinct from conventional luciferase has been desired. A luciferase mutant comprising an amino acid sequence in which glutamic acid at position 4, arginine at position 11, leucine at position 18 and valine at position 27 are substituted with other amino acids, in the amino acid sequence of SEQ ID NO: 2.Type: ApplicationFiled: December 7, 2017Publication date: May 3, 2018Applicant: JNC CORPORATIONInventors: Satoshi INOUYE, Yuiko MIURA, Junichi SATO
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Patent number: 9868941Abstract: A novel luciferase that is distinct from conventional luciferase has been desired. A luciferase mutant comprising an amino acid sequence in which glutamic acid at position 4, arginine at position 11, leucine at position 18 and valine at position 27 are substituted with other amino acids, in the amino acid sequence of SEQ ID NO: 2.Type: GrantFiled: April 14, 2015Date of Patent: January 16, 2018Assignee: JNC CORPORATIONInventors: Satoshi Inouye, Yuiko Miura, Junichi Sato
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Publication number: 20170121755Abstract: Coelenterazine analogs having luminescence properties different from those of known coelenterazine analogs are desired for various luciferases. The invention provides compounds represented by general formula (1) below.Type: ApplicationFiled: December 29, 2016Publication date: May 4, 2017Inventors: Satoshi INOUYE, Junichi SATO, Yuiko MIURA
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Patent number: 9567623Abstract: Coelenterazine analogs having luminescence properties different from those of known coelenterazine analogs are desired for various luciferases. The invention provides compounds represented by general formula (1) below.Type: GrantFiled: April 4, 2014Date of Patent: February 14, 2017Assignee: JNC CORPORATIONInventors: Satoshi Inouye, Junichi Sato, Yuiko Miura
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Patent number: 9346767Abstract: Described include (1) a substituted pyrazine compound of general formula (VIII): and (2) a process for producing the substituted pyrazine compound of general formula (VIII), where R1 is hydrogen, a halogen, a substituted or unsubstituted hydrocarbon group, or a substituted or unsubstituted heterocyclic group, and R4 is a protecting group. The process comprises (1) the step of reacting 2-amino-3,5-dibromo-6-chloropyrazine with R1MgX and ZnCl2 in the presence of a palladium catalyst to produce a compound of general formula (V); (2) the step of reacting the compound of general formula (V) with a compound of general formula (VI) to produce a compound of general formula (VII); and (3) the step of reacting the compound of formula (VII) with tributyl(vinyl)tin in the presence or a palladium catalyst to given the compound of general formula (VIII).Type: GrantFiled: June 9, 2015Date of Patent: May 24, 2016Assignees: JNC CORPORATION, NATIONAL UNIVERSITY CORPORATION TOKYO MEDICAL AND DENTAL UNIVERSITYInventors: Satoshi Inouye, Yuiko Miura, Suguru Yoshida, Takamitsu Hosoya
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Patent number: 9238681Abstract: Calcium-binding photoproteins showing the luminescence pattern with a slow decay of are desired. The invention provides a mutant apoprotein comprising an amino acid sequence wherein the 23rd to 34th amino acids in the amino acid sequence of SEQ ID NO: 2 are substituted with an amino acid represented by formula I below: Xaa23-Xaa24-Xaa25-Xaa26-Xaa27-Xaa28-Xaa29-Xaa30-Xaa31-Xaa32-Xaa33-Xaa 34; having a function to bind to the peroxide of coelenterazine or the peroxide of a coelenterazine analog to form a photoprotein capable of emitting light under the action of calcium ions; and, having a half decay time of the luminescence emitted by binding of the photoprotein to calcium ions being not less than 2 seconds.Type: GrantFiled: June 17, 2014Date of Patent: January 19, 2016Assignee: JNC CORPORATIONInventors: Satoshi Inouye, Yuiko Miura
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Publication number: 20150299675Abstract: A novel luciferase that is distinct from conventional luciferase has been desired. A luciferase mutant comprising an amino acid sequence in which glutamic acid at position 4, arginine at position 11, leucine at position 18 and valine at position 27 are substituted with other amino acids, in the amino acid sequence of SEQ ID NO: 2.Type: ApplicationFiled: April 14, 2015Publication date: October 22, 2015Inventors: Satoshi INOUYE, Yuiko MIURA, Junichi SATO
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Publication number: 20150266833Abstract: A simple process for producing ?-coelenterazine compounds has been desired. Described is a process for producing a ?-coelenterazine compound represented by general formula (II) comprising (1) the step of reacting a compound of general formula (VIII) with a methyltriphenylphosphonium salt in the presence of a base to give a compound represented by general formula (IX), (2) the step of performing a ring-closing metathesis reaction on any one selected from the group consisting of the compound represented by general formula (IX) and a compound of general formula (X) which is the compound of general formula (IX) wherein the amino is protected with R5, and then deprotecting R4 and, if any, R5 to give a ?-coelenteramine compound represented by general formula (XIV), and (3) the step of reacting the compound of general formula (XIV) with a compound represented by general formula (XV) to give the compound of general formula (II).Type: ApplicationFiled: June 9, 2015Publication date: September 24, 2015Inventors: Satoshi INOUYE, Yuiko MIURA, Suguru YOSHIDA, Takamitsu HOSOYA
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Publication number: 20150232519Abstract: Calcium-binding photoproteins showing the luminescence pattern with a slow decay of are desired. The invention provides a mutant apoprotein comprising an amino acid sequence wherein the 23rd to 34th amino acids in the amino acid sequence of SEQ ID NO: 2 are substituted with an amino acid represented by formula I below: Xaa23-Xaa24-Xaa25-Xaa26-Xaa27-Xaa28-Xaa29-Xaa30-Xaa31-Xaa32-Xaa33-Xaa 34; having a function to bind to the peroxide of coelenterazine or the peroxide of a coelenterazine analogue to form a photoprotein capable of emitting light under the action of calcium ions; and, having a half decay time of the luminescence emitted by binding of the photoprotein to calcium ions being not less than 2 seconds.Type: ApplicationFiled: June 17, 2014Publication date: August 20, 2015Inventors: Satoshi INOUYE, Yuiko MIURA
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Publication number: 20150119575Abstract: A simple process for producing ?-coelenterazine compounds has been desired. The invention provides a process for producing a ?-coelenterazine compound represented by general formula (II) which comprises (1) the step of reacting a compound represented by general formula (VIII) with a methyltriphenylphosphonium salt in the presence of a base to give a compound represented by general formula (IX), (2) the step of performing a ring-closing metathesis reaction on any one selected from the group consisting of the compound represented by general formula (IX) and a compound represented by general formula (X) which is the compound represented by general formula (IX) wherein the amino is protected with R5, and then deprotecting R4 and, if any, R5 to give a ?-coelenteramine compound represented by general formula (XIV), and (3) the step of reacting the compound represented by general formula (XIV) with a compound represented by general formula (XV) to give the compound represented by general formula (II).Type: ApplicationFiled: January 8, 2015Publication date: April 30, 2015Inventors: SATOSHI INOUYE, YUIKO MIURA, SUGURU YOSHIDA, TAKAMITSU HOSOYA
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Patent number: 8975403Abstract: A simple process for producing v-coelenterazine compounds has been desired. Described is a process for producing a v-coelenterazine compound represented by general formula (II) comprising (1) the step of reacting a compound of general formula (VIII) with a methyltriphenylphosphonium salt in the presence of a base to give a compound represented by general formula (IX), (2) the step of performing a ring-closing metathesis reaction on any one selected from the group consisting of the compound represented by general formula (IX) and a compound of general formula (X) which is the compound of general formula (IX) wherein the amino is protected with R5, and then deprotecting R4 and, if any, R5 to give a v-coelenteramine compound represented by general formula (XIV), and (3) the step of reacting the compound of general formula (XIV) with a compound represented by general formula (XV) to give the compound of general formula (II).Type: GrantFiled: February 25, 2014Date of Patent: March 10, 2015Assignees: JNC Corporation, National University Corporation Tokyo Medical and Dental UniversityInventors: Satoshi Inouye, Yuiko Miura, Suguru Yoshida, Takamitsu Hosoya
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Publication number: 20140302539Abstract: Coelenterazine analogs having luminescence properties different from those of known coelenterazine analogs are desired for various luciferases. The invention provides compounds represented by general formula (1) below.Type: ApplicationFiled: April 4, 2014Publication date: October 9, 2014Applicant: JNC CORPORATIONInventors: Satoshi INOUYE, Junichi SATO, Yuiko MIURA
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Patent number: 8816052Abstract: Calcium-binding photoproteins showing the luminescence pattern with a slow decay of are desired. The invention provides a mutant apoprotein comprising an amino acid sequence wherein the 23rd to 34th amino acids in the amino acid sequence of SEQ ID NO: 2 are substituted with an amino acid represented by formula I below: Xaa23-Xaa24-Xaa25-Xaa26-Xaa27-Xaa28-Xaa29-Xaa30-Xaa31-Xaa32-Xaa33-Xaa 34; having a function to bind to the peroxide of coelenterazine or the peroxide of a coelenterazine analogue to form a photoprotein capable of emitting light under the action of calcium ions; and, having a half decay time of the luminescence emitted by binding of the photoprotein to calcium ions being not less than 2 seconds.Type: GrantFiled: March 27, 2012Date of Patent: August 26, 2014Assignee: JNC CorporationInventors: Satoshi Inouye, Yuiko Miura
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Publication number: 20140206868Abstract: A simple process for producing v-coelenterazine compounds has been desired. Described is a process for producing a v-coelenterazine compound represented by general formula (II) comprising (1) the step of reacting a compound of general formula (VIII) with a methyltriphenylphosphonium salt in the presence of a base to give a compound represented by general formula (IX), (2) the step of performing a ring-closing metathesis reaction on any one selected from the group consisting of the compound represented by general formula (IX) and a compound of general formula (X) which is the compound of general formula (IX) wherein the amino is protected with R5, and then deprotecting R4 and, if any, R5 to give a v-coelenteramine compound represented by general formula (XIV), and (3) the step of reacting the compound of general formula (XIV) with a compound represented by general formula (XV) to give the compound of general formula (II).Type: ApplicationFiled: February 25, 2014Publication date: July 24, 2014Applicants: NATIONAL UNIVERSITY CORPORATION TOKYO MEDICAL AND DENTAL UNIVERSITY, JNC CORPORATIONInventors: Satoshi INOUYE, Yuiko MIURA, Suguru YOSHIDA, Takamitsu HOSOYA
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Patent number: 8772484Abstract: A simple process for producing v-coelenterazine compounds has been desired. Described is a process for producing a v-coelenterazine compound represented by general formula (II) comprising (1) the step of reacting a compound of general formula (VIII) with a methyltriphenylphosphonium salt in the presence of a base to give a compound represented by general formula (IX), (2) the step of performing a ring-closing metathesis reaction on any one selected from the group consisting of the compound represented by general formula (IX) and a compound of general formula (X) which is the compound of general formula (IX) wherein the amino is protected with R5, and then deprotecting R4 and, if any, R5 to give a v-coelenteramine compound represented by general formula (XIV), and (3) the step of reacting the compound of general formula (XIV) with a compound represented by general formula (XV) to give the compound of general formula (II).Type: GrantFiled: March 6, 2012Date of Patent: July 8, 2014Assignees: JNC Corporation, National University Corporation Tokyo Medical and Dental UniversityInventors: Satoshi Inouye, Yuiko Miura, Suguru Yoshida, Takamitsu Hosoya
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Publication number: 20120258465Abstract: Calcium-binding photoproteins showing the luminescence pattern with a slow decay of are desired. The invention provides a mutant apoprotein comprising an amino acid sequence wherein the 23rd to 34th amino acids in the amino acid sequence of SEQ ID NO: 2 are substituted with an amino acid represented by formula I below: Xaa23-Xaa24-Xaa25-Xaa26-Xaa27-Xaa28-Xaa29-Xaa30-Xaa31-Xaa32-Xaa33-Xaa 34; having a function to bind to the peroxide of coelenterazine or the peroxide of a coelenterazine analogue to form a photoprotein capable of emitting light under the action of calcium ions; and, having a half decay time of the luminescence emitted by binding of the photoprotein to calcium ions being not less than 2 seconds.Type: ApplicationFiled: March 27, 2012Publication date: October 11, 2012Applicant: JNC CORPORATIONInventors: Satoshi INOUYE, Yuiko Miura
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Publication number: 20120232272Abstract: A simple process for producing v-coelenterazine compounds has been desired. Described is a process for producing a v-coelenterazine compound represented by general formula (II) comprising (1) the step of reacting a compound of general formula (VIII) with a methyltriphenylphosphonium salt in the presence of a base to give a compound represented by general formula (IX), (2) the step of performing a ring-closing metathesis reaction on any one selected from the group consisting of the compound represented by general formula (IX) and a compound of general formula (X) which is the compound of general formula (IX) wherein the amino is protected with R5, and then deprotecting R4 and, if any, R5 to give a v-coelenteramine compound represented by general formula (XIV), and (3) the step of reacting the compound of general formula (XIV) with a compound represented by general formula (XV) to give the compound of general formula (II).Type: ApplicationFiled: March 6, 2012Publication date: September 13, 2012Applicants: NATIONAL UNIVERSITY CORPORATION TOKYO MEDICAL AND DENTAL UNIVERSITY, JNC CORPORATIONInventors: Satoshi INOUYE, Yuiko Miura, Suguru Yoshida, Takamitsu Hosoya