Abstract: A fluorescence detection apparatus irradiates a sample with an excitation light having different wavelengths, spectrally detects fluorescence from the sample, and includes: a comparison unit for comparing the wavelength of the excitation light with the starting wavelength of a fluorescent spectrum detection bandwidth; and a determination unit for determining an excitation light irradiation timing such that the irradiation of the excitation wavelength is stopped when the wavelength of the excitation light is longer than the starting wavelength.

Abstract: A light deflector comprises a first movable plate having a reflective surface, fixing portions, a pair of second movable plates located between the first movable plate and the fixing portions, a pair of first elastic members supporting the first movable plate so as to allow it to oscillate with respect to the second movable plates, a pair of second elastic members supporting the second movable plates so as to allow them to oscillate with respect to the fixing portions, and an actuator to oscillate the first movable plate. Oscillation axis of the first elastic members and oscillation axis of the second elastic members are located on the same axis. Stiffness of the second elastic members against oscillation is lower than stiffness of the first elastic members against oscillation.

Abstract: A scanning laser microscope includes a laser generation unit which generates a laser beam, a light modulation unit which modulates the laser beam, a scanning unit which scans a sample, a light receiving unit which receives a light from the sample, a control unit which controls the light modulation unit for each pixel of a scanning image acquired by the light receiving of the light receiving unit, and a storage unit which stores a plurality of controlled patterns. The number of pixels of the patterns correspond to the number of pixels of the scanned image. A modulation information of the laser beam is set for each pixel. The control unit reads the control patterns stored in the storage unit, controls the light modulation unit based on the modulation information of each pixel, and performs such control that the control patterns are changed at an arbitrary timing.

Abstract: A scanning laser microscope includes a laser generation unit which generates a laser beam, a light modulation unit which modulates the laser beam, a scanning unit which scans a sample, a light receiving unit which receives a light from the sample, a control unit which controls the light modulation unit for each pixel of a scanning image acquired by the light receiving of the light receiving unit, and a storage unit which stores a plurality of controlled patterns. The number of pixels of the patterns correspond to the number of pixels of the scanned image. A modulation information of the laser beam is set for each pixel. The control unit reads the control patterns stored in the storage unit, controls the light modulation unit based on the modulation information of each pixel, and performs such control that the control patterns are changed at an arbitrary timing.

Abstract: A scanning laser microscope includes a laser generation unit which generates a laser beam, a light modulation unit which modulates the laser beam, a scanning unit which scans a sample, a light receiving unit which receives a light from the sample, a control unit which controls the light modulation unit for each pixel of a scanning image acquired by the light receiving of the light receiving unit, and a storage unit which stores a plurality of controlled patterns. The number of pixels of the patterns correspond to the number of pixels of the scanned image. A modulation information of the laser beam is set for each pixel. The control unit reads the control patterns stored in the storage unit, controls the light modulation unit based on the modulation information of each pixel, and performs such control that the control patterns are changed at an arbitrary timing.

Abstract: A scanning laser microscope includes a laser generation unit which generates a laser beam, a light modulation unit which modulates the laser beam, a scanning unit which scans a sample, a light receiving unit which receives a light from the sample, a control unit which controls the light modulation unit for each pixel of a scanning image acquired by the light receiving of the light receiving unit, and a storage unit which stores a plurality of controlled patterns. The number of pixels of the patterns correspond to the number of pixels of the scanned image. A modulation information of the laser beam is set for each pixel. The control unit reads the control patterns stored in the storage unit, controls the light modulation unit based on the modulation information of each pixel, and performs such control that the control patterns are changed at an arbitrary timing.

Abstract: An image data acquisition method comprises scanning a sample by a light, receiving a light from the sample, to acquire a scanned image data, and storing the scanned image data obtained by scanning a region of a predetermined size every time a region scanned by the light reaches a predetermined size, sequentially.

Abstract: A color chart where hue and saturation are changed variously with lightness kept constant is outputted onto a CRT monitor (or a color printer) beforehand. The display color corresponding to a specific wavelength of fluorescence in visual observation is specified on the color chart. The hue and saturation for the display color at that time are stored as color conversion parameters in a data processing unit. Thereafter, the color tone of the one with a specific wavelength of fluorescence among the luminance data of fluorescence of the observed specimen is corrected using the color conversion parameters. The resulting observed image is outputted to the CRT monitor (or color printer). This enables color reproduction of the observed image easily.

Abstract: A laser scan microscope irradiates a sample with a laser beam while simultaneously scanning the laser beam over the sample, and processes an optical signal obtained thereby so as to obtain image data. The laser scan microscope is provided with a photoelectric converting unit, a sensitivity adjuster, and a sampling/converting unit. The photoelectric converting unit converts an optical signal from the sample into an electric signal. The sensitivity adjuster adjusts the sensitivity of the photoelectric converter such that an output value of the photoelectric converting unit becomes closer to a predetermined reference value. The sampling/converting unit samples sensitivity information determined for the photoelectric converter, in accordance with a scanning speed, and converts the sampled sensitivity information into a light measurement value used for obtaining the image data.