Abstract: A test strip and kit for testing mycophenolic acid and a preparation method of the test strip are described. The test strip includes a bottom plate and a sample pad, a glassfiber membrane, a nitrocellulose membrane and an absorbent paper which are successively lapped on a surface of the bottom plate, the sample pad is treated by a sample pad treatment fluid; the glassfiber membrane is treated by a glassfiber membrane treatment fluid; the glassfiber membrane is coated by a mycophenolic acid specific-antibody conjugate; the nitrocellulose membrane is provided with a detection line and a quality control line; and a mycophenolic acid protein conjugate is sprayed on the detection line.

Abstract: Provided are a method and device for creating a gene mutation dictionary, and a method and device for compressing genomic data using the gene mutation dictionary. The method for creating a gene mutation dictionary includes: obtaining genome sequence data of a plurality of individuals of a species and reference genome data of the species; aligning genome sequence data of each individual to the reference genome data to obtain a mutation result of the genome sequence data of each individual relative to the reference genome data; partitioning a genome of the species into a plurality of unit regions of biological significance; and generating a plurality of mutant patterns of the individuals in each unit region by statistically analyzing mutant status for each unit region based on the mutation result, and numbering the mutant patterns, to obtain the gene mutation dictionary.

Abstract: Provided are a Phi29 DNA polymerase mutant with improved thermal stability and an use thereof in sequencing. The phi29 DNA polymerase mutant is represented by A) or B) below: the DNA polymerase mutant represented by the A) is a protein having DNA polymerase activity that is obtained by modifying at least one amino acid residue(s) in the following six sites in a phi29 DNA polymerase amino acid sequence: the 97-th, 123-th, 217-th, 224-th, 515-th, and 474-th sites; the DNA polymerase mutant represented by the B) is a protein having DNA polymerase activity that is derived from the A) by adding a tag sequence to a terminal of the amino acid sequence of the protein represented by the A).

Abstract: A detection kit for quickly detecting a lead content lead a sample is provided, and belongs to the technical field of medical in vitro immunoassay. The kit includes a detection card and a quality control; the detection card includes a bottom plate, and a sample pad, a glass fiber membrane, a nitrocellulose membrane and an absorbent paper which are arranged on the surface of the bottom plate sequentially from a loading end. The provided kit has advantages of having simple preprocessing, having no need of digestion, and being economic, fast and convenient, and the like, can realize storage at room temperature, a rapid speed, a high throughout, a low instrument cost, simple and convenient operations, single-person packaging, and detection at any time, which greatly improves the simplicity and convenience of clinical use.

Abstract: Provided are a group of phi29 DNA polymerase mutants having increased thermal stability and use thereof. The phi29 DNA polymerase mutants are proteins obtained by performing point mutation A and/or point mutation B and/or point mutation C on phi29 DNA polymerase, the point mutation A meaning that an amino acid residue M at position 97 of the phi29 DNA polymerase is mutated to other amino acid residue, the point mutation B meaning that an amino acid residue L at position 123 of the phi29 DNA polymerase is mutated into other amino acid residue, and the point mutation C meaning that an amino acid residue E at position 515 of the phi29 DNA polymerase is mutated to other amino acid residue. The stability of the phi29 DNA polymerase mutants is higher than that of a wild-type phi29 DNA polymerase.

Abstract: The present invention relates to a detection kit for quickly detecting a cadmium content in a sample, and belongs to the technical field of medical in vitro immunoassay. The kit of the present invention includes a detection card and a quality control; the detection card includes a bottom plate, and a sample pad, a glass fiber membrane, a nitrocellulose membrane and an absorbent paper which are arranged on the surface of the bottom plate sequentially from a loading end. The kit of the present invention has advantages of a low detection instrument cost, simple and rapid operations, storage and transportation at room temperature, realization of packaging for a single person, and a good stability.

Abstract: A detection kit for quickly detecting a lead content lead a sample is provided, and belongs to the technical field of medical in vitro immunoassay. The kit includes a detection card and a quality control; the detection card includes a bottom plate, and a sample pad, a glass fiber membrane, a nitrocellulose membrane and an absorbent paper which are arranged on the surface of the bottom plate sequentially from a loading end. The provided kit has advantages of having simple preprocessing, having no need of digestion, and being economic, fast and convenient, and the like, can realize storage at room temperature, a rapid speed, a high throughout, a low instrument cost, simple and convenient operations, single-person packaging, and detection at any time, which greatly improves the simplicity and convenience of clinical use.

Abstract: Provided are a phi29 DNA polymerase mutant with increased thermo stability, a method for preparing the mutant, the use of the mutant, and a method for increasing the stability of the phi29 DNA polymerase.

Abstract: The invention provides immunochromatographic test strips for detecting digoxin and uses thereof. The immunochromatographic test strips include a bottom plate, a sample pad, a glass fiber mat, a nitrocellulose membrane and a water absorbing paper that are sequentially overlapped on the bottom plate. The glass fiber mat is sprayed with a digoxin-specific antibody-fluorescent microsphere complex. The nitrocellulose membrane is sequentially provided with a detection line coated with digoxin protein conjugate and a quality control line coated with a secondary antibody. The digoxin-specific antibody-fluorescent microsphere complex is obtained by conjugation between a digoxin monoclonal antibody or a digoxin polyclonal antibody and a fluorescent microsphere. The digoxin protein conjugate includes a digoxin-conjugated rabbit albumin or a digoxin-conjugated rabbit ovalbumin. The test strips provided by the invention are low cost, provide simple and rapid use, and have excellent stability and high sensitivity.

Abstract: A test strip and kit for testing mycophenolic acid and a preparation method of the test strip are described. The test strip includes a bottom plate and a sample pad, a glassfiber membrane, a nitrocellulose membrane and an absorbent paper which are successively lapped on a surface of the bottom plate, the sample pad is treated by a sample pad treatment fluid; the glassfiber membrane is treated by a glassfiber membrane treatment fluid; the glassfiber membrane is coated by a mycophenolic acid specific-antibody conjugate; the nitrocellulose membrane is provided with a detection line and a quality control line; and a mycophenolic acid protein conjugate is sprayed on the detection line.

Abstract: Provided are a group of phi29 DNA polymerase mutants having increased thermal stability and use thereof. The phi29 DNA polymerase mutants are proteins obtained by performing point mutation A and/or point mutation B and/or point mutation C on phi29 DNA polymerase, the point mutation A meaning that an amino acid residue M at position 97 of the phi29 DNA polymerase is mutated to other amino acid residue, the point mutation B meaning that an amino acid residue L at position 123 of the phi29 DNA polymerase is mutated into other amino acid residue, and the point mutation C meaning that an amino acid residue E at position 515 of the phi29 DNA polymerase is mutated to other amino acid residue. The stability of the phi29 DNA polymerase mutants is higher than that of a wild-type phi29 DNA polymerase.

Abstract: Provided are a phi29 DNA polymerase mutant with increased thermo stability, a method for preparing the mutant, the use of the mutant, and a method for increasing the stability of the phi29 DNA polymerase.