Abstract: An object of the present invention is to provide a cell culture device capable of reproducing, in vitro, a growth and proliferation environment of an anaerobic bacterium in a living body; a kit for cell culture; and a cell culture method. According to the present invention, there is provided a cell culture device including a plate having a concave portion for accommodating a culture medium, a cell accommodating container having a gas permeable membrane, a first member for holding the cell accommodating container, and a second member having a structure for regulating a gas concentration in an upper space of the cell accommodating container, in which, in a case where a cell sheet is formed on an upper surface of the gas permeable membrane, a space above the gas permeable membrane is in a sealed state in which gas does not pass through.

Abstract: An object of the present invention is to provide a method of producing an enteroendocrine cell by using an intestinal stem cell induced from a pluripotent stem cell, a method of analyzing a disease mechanism, an evaluation method for a test substance, a screening method for a therapeutic drug for a digestive system disease, an enteroendocrine cell derived from a pluripotent stem cell, a culture medium or a culture medium kit, and a kit for producing an enteroendocrine cell. According to the present invention, there is provided a method of producing an enteroendocrine cell, the method including an intestinal stem cell or an intestinal epithelial cell, which is induced from a pluripotent stem cell, by using a culture medium that substantially does not contain a ligand of an epidermal growth factor but contains a cAMP activator and a DNA methyltransferase inhibitor.

Abstract: An object of the present invention is to provide a method of producing an intestinal epithelial cell, which has a large number of cells per area and a high accuracy of kinetic prediction for a CYP3A4 substrate drug such as midazolam, by inducing the differentiation of a pluripotent stem cell, as well as the intestinal epithelial cell, a cell sheet, an evaluation method for a test substance, a screening kit for a test substance, and a cell preparation.

Abstract: An object of the present invention is to provide a transportation instrument and a transportation method that enable transportation by using a cell culture container as it is. Further, an object of the present invention is to provide a transportation instrument and a transportation method that enable supply of oxygen required for respiration of cells. Further, an object of the present invention is to provide a transportation instrument and a transportation method that enable suppression of migration of a culture medium into and out of cells caused by the influence of an increase in internal pressure with respect to the temperature during transportation.

Abstract: An object of the present invention is to provide a method for producing an intestinal epithelial cell in which the barrier function is maintained while the differentiation of a pluripotent stem cell into a liver cell is suppressed and an intestinal epithelial cell in which the barrier function is maintained while the differentiation into a liver cell is suppressed. According to the present invention, provided is the method for producing an intestinal epithelial cell, including a step 1 of differentiating a pluripotent stem cell into an intestinal stem cell and a step 2 of differentiating the intestinal stem cell obtained in the step 1 into an intestinal epithelial cell in a presence of one or more selected from the group consisting of a MEK1 inhibitor, a DNA methylation inhibitor, and a TGF? receptor inhibitor, and EGF, in which during the step 2, a cell under differentiation is replated one or more times at the predetermined timing which is defined in the present specification.