Patents by Inventor Yukihiro Yabuta

Yukihiro Yabuta has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Nucleic acid sequence amplification method
    Patent number: 11028426
    Abstract: The invention provides a method of preparing a nucleic acid population suitable for RNA sequencing. The method involves amplifying a double-stranded DNA and a poly T sequence by using the DNA constituted of any additional nucleic acid sequence X, poly T sequence, mRNA sequence isolated from a biological sample, poly A sequence and any additional nucleic acid sequence Y in this order as a template, a first primer containing any additional nucleic acid sequence X having amine added to the 5?-terminal (and a poly T sequence), and a second primer containing any additional nucleic acid sequence Y (and a poly T sequence), followed by fractionalizing the DNA, phosphorylating the DNA, preparing cDNA by using the DNA as a template and a third primer, adding adenine (A) to the cDNA, linking a DNA, and amplifying the DNA by using the DNA as a template, a fourth primer, and a fifth primer.
    Type: Grant
    Filed: February 23, 2016
    Date of Patent: June 8, 2021
    Assignee: KYOTO UNIVERSITY
    Inventors: Mitinori Saitou, Tomonori Nakamura, Yukihiro Yabuta
  • NUCLEIC ACID SEQUENCE AMPLIFICATION METHOD
    Publication number: 20180044714
    Abstract: The invention provides a method of preparing a nucleic acid population suitable for RNA sequencing. The method involves amplifying a double-stranded DNA and a poly T sequence by using the DNA constituted of any additional nucleic acid sequence X, poly T sequence, mRNA sequence isolated from a biological sample, poly A sequence and any additional nucleic acid sequence Y in this order as a template, a first primer containing any additional nucleic acid sequence X having amine added to the 5?-terminal (and a poly T sequence), and a second primer containing any additional nucleic acid sequence Y (and a poly T sequence), followed by fractionalizing the DNA, phosphorylating the DNA, preparing cDNA by using the DNA as a template and a third primer, adding adenine (A) to the cDNA, linking a DNA, and amplifying the DNA by using the DNA as a template, a fourth primer, and a fifth primer.
    Type: Application
    Filed: February 23, 2016
    Publication date: February 15, 2018
    Applicant: KYOTO UNIVERSITY
    Inventors: Mitinori SAITOU, Tomonori NAKAMURA, Yukihiro YABUTA
  • Method for amplification of nucleotide sequence
    Patent number: 9222129
    Abstract: A method for amplification of a nucleotide sequence characterized by performing PCR amplification using mRNA isolated from a biological sample as a template and using a first primer comprising a poly(T) sequence and an additional nucleotide sequence X thereto and a second primer comprising a poly(T) sequence and an additional nucleotide sequence Y thereto, provided that the nucleotide sequences X and Y in the first and second primers, respectively, have different sequences from each other.
    Type: Grant
    Filed: February 10, 2006
    Date of Patent: December 29, 2015
    Assignee: RIKEN
    Inventors: Mitinori Saitou, Kazuki Kurimoto, Yukihiro Yabuta, Yasuhide Ohinata
  • Method for Amplification of Nucleotide Sequence
    Publication number: 20090291852
    Abstract: A method for amplification of a nucleotide sequence characterized by performing PCR amplification using mRNA isolated from a biological sample as a template and using a first primer comprising a poly(T) sequence and an additional nucleotide sequence X thereto and a second primer comprising a poly(T) sequence and an additional nucleotide sequence Y thereto, provided that the nucleotide sequences X and Y in the first and second primers, respectively, have different sequences from each other.
    Type: Application
    Filed: February 10, 2006
    Publication date: November 26, 2009
    Applicant: Riken
    Inventors: Mitinori Saitou, Kazuki Kurimoto, Yukihiro Yabuta, Yasuhide Ohinata