Abstract: A mutant MAD7 protein with higher target-site cleavage activity is provided. The mutant MAD7 protein includes an amino acid sequence B obtained by introducing mutations into an amino acid sequence A represented by SEQ ID NO:1, the amino acid sequence B including amino-acid substitution mutations of K169 and D529 in the amino acid sequence A.

Abstract: The present invention establishes a molecular therapy for glycogen storage disease type Ia. The present invention provides an oligonucleotide of 15-30 bases comprising a nucleotide sequence complementary to die cDNA of G6PC gene with c.648G>T mutation, wherein the oligonucleotide comprises a sequence complementary to a region comprising any site between the 82nd to the 92nd nucleotide from the 5? end of exon 5 of the G6PC gene with c.648C>T mutation, a pharmacologically acceptable salt or solvate thereof. Also provided is a pharmaceutical drug comprising the oligonucleotide, a pharmacologically acceptable salt or solvate thereof (e.g., therapeutic drug for glycogen storage disease type Ia).

Abstract: The process which comprises causing cells or a culture of a microorganism having the activity to introduce hydroxy into the [n-5]-position (n is an even number of 10 or more) and hydrogen into the [n-6]-position of a straight-chain fatty acid having n carbon atoms wherein at least the [n-6]-position is a double bond to make the [n-6]-position a single bond or a treated matter thereof to act on a straight-chain fatty acid having n carbon atoms wherein at least the [n-6]-position is a double bond or a composition containing the fatty acid to form an [n-5]-hydroxy fatty acid wherein the [n-6]-position is a single bond, causing cells or a culture of a microorganism having the activity to &bgr;-oxidize an [n-5]-hydroxy fatty acid wherein the [n-6]-position is a single bond or a treated matter thereof to act on the formed [n-5]-hydroxy fatty acid, and recovering the formed &dgr;-lactones.

Abstract: The process which comprises causing cells or a culture of a microorganism having the activity to introduce hydroxy into the [n-5]-position (n is an even number of 10 or more) and hydrogen into the [n-6]-position of a straight-chain fatty acid having n carbon atoms wherein at least the [n-6]-position is a double bond to make the [n-6]-position a single bond or a treated matter thereof to act on a straight-chain fatty acid having n carbon atoms wherein at least the [n-6]-position is a double bond or a composition containing the fatty acid to form an [n-5]-hydroxy fatty acid wherein the [n-6]-position is a single bond, causing cells or a culture of a microorganism having the activity to &bgr;-oxidize an [n-5]-hydroxy fatty acid wherein the [n-6 ]-position is-a single bond or a treated matter thereof to act on the formed [n-5]-hydroxy fatty acid, and recovering the formed &dgr;-lactones.