Abstract: Provided is a detection method employing a detection instrument having a plurality of wells at a surface thereof, the method including depositing a detection object, preferably in coexistence with a specific holding substance, onto the inner walls of the wells of the detection instrument; bringing a reaction solution into contact with the inner walls of the wells of the detection instrument on which the detection object has been deposited, the reaction solution containing a substance reactive with the detection object; and detecting a signal generated through this contact, to thereby evaluate the detection object. In this method, a liquid phase reaction can be efficiently performed in the detection instrument for carrying out a biological reaction (e.g., a microarray) so that samples contained in the wells are not mixed with one another, and that air bubbles are prevented from being generated in the wells.

Abstract: There are provided a) a detection board having numerous wells on a surface thereof, b) a detection set including the detection board and an element serving as well sealing means, and c) a method of using the detection set b), which includes injecting, into each of the wells of the detection board, an element for detecting a liquid phase reaction, sealing the element into the well with the well sealing means, and detecting the liquid phase reaction in the well. Thus, there is provided means for efficiently performing a liquid phase reaction on the board in a manner similar to that of an existing microarray technique.

Abstract: A method of detecting a risk factor for the onset of arteriosclerosis in a subject on the basis of correlation of a gene mutation in the CETP gene (in particular, mutation at (1) guanine at the −69-position of the CETP gene; (2) the site encoding leucine at the 151-position of CETP encoded by the CETP gene; (3) the site encoding arginine at the 201-position and alanine at the 202-position of the same; or (4) the site encoding arginine at the 282-position of the same) with the risk for arteriosclerosis, thereby detecting arteriosclerosis.

Abstract: Risk factors for diabetic onset in a subject are detected by using a gene coding for CD38 protein, which is originally identified as a human lymphocyte surface marker, as a risk gene, and detecting mutations in the gene.

Abstract: Risk factors for diabetic onset in a subject are detected by using a gene coding for CD38 protein, which is originally identified as a human lymphocyte surface marker, as a risk gene, and detecting mutations in the gene.