Patents by Inventor Yukimitsu Nakagawa

Yukimitsu Nakagawa has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Mutant AOX2 promoter, microorganism carrying same, method of preparation thereof and production of heterologous protein using such microorganism
    Patent number: 5610036
    Abstract: A mutant AOX2 (alcohol oxidase 2) promoter derived from the natural AOX2 promoter by base sequence deletion, insertion, or substitution is disclosed. A microbial strain carrying a gene under the control of such a mutant AOX2 promoter can be obtained by growing in a methanol-containing medium a strain incapable of producing AOX encoded by the AOX1 gene but carrying the AOX2 gene under the control of the natural AOX2 promoter. A heterologous protein may be produced by cultivating the mutant strain with the desired heterologous protein gene incorporated downstream from the mutant AOX2 promoter. A plasmid carrying the mutant AOX2 promoter is also provided.
    Type: Grant
    Filed: August 10, 1994
    Date of Patent: March 11, 1997
    Assignee: The Green Cross Corporation
    Inventors: Masami Miura, Yutaka Ishida, Hideyuki Oi, Koji Murakami, Yukimitsu Nakagawa, Haruhide Kawabe
  • Method of preparing foreign protein in yeast, recombinant DNA, transformant
    Patent number: 5503993
    Abstract: A method for preparing foreign protein in yeast using an expression recombinant DNA comprising DNA encoding the serum albumin signal peptide adjacent to DNA encoding the foreign protein is disclosed.
    Type: Grant
    Filed: May 22, 1995
    Date of Patent: April 2, 1996
    Assignee: The Green Cross Corporation
    Inventors: Naofumi Hayasuke, Yukimitsu Nakagawa, Yutaka Ishida, Ken Okabayashi, Kohji Murakami, Kiyoshi Tsutsui, Kazuo Ikegaya, Hitoshi Minamino, Sadao Ueda, Haruhide Kawabe, Hirofumi Arimura, Atusi Masaki
  • DNA's encoding signal peptides
    Patent number: 5409815
    Abstract: Novel signal peptides capable of functioning in yeasts have the amino acid sequence:Met-A.sub.1 -A.sub.2 -X-B-C-D-E-Fwherein A.sub.1 is a peptide chain composed of 1-3 amino acids each selected from the group consisting of Arg, Ser, Lys and His, A.sub.2 is a peptide chain composed of 1-3 amino acids, X is a peptide chain composed of 8-10 hydrophobic amino acids, B is Pro or Ser, C is Gly or Pro, D Cys, Ala, Leu, Ser, Thr and Val, E is Trp or Gln and F is Ala or Gly. DNA sequences coding for these signal peptides can be used for the secretory expression of heterologous proteins in yeasts.
    Type: Grant
    Filed: February 16, 1989
    Date of Patent: April 25, 1995
    Assignee: The Green Cross Corporation
    Inventors: Yukimitsu Nakagawa, Naofumi Hayasuke, Atsushi Masaki, Yutaka Ishida, Kohji Murakami, Ken Okabayashi, Kiyoshi Tsutsui, Hitoshi Minamino, Kazuo Ikegaya, Kaoru Kobayashi, Haruhide Kawabe, Sadao Ueda