Patents by Inventor Yukinobu Hayashida
Yukinobu Hayashida has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11162995Abstract: According to one embodiment, an arithmetic processor generates unknown code distribution information, for an unknown test code to which no failure mode has been specified, and determines whether labeling of the unknown code distribution information is performable. The arithmetic processor determines whether the unknown code distribution information agrees to known code distribution information, when labeling of the unknown code distribution information is performable. The arithmetic processor determines whether the unknown code satisfies a classification minimum requirement of the agreed known code. The arithmetic processor presents a content of EFA measurement that assumes a failure mode corresponding to the known code, when the unknown code distribution information agrees to the known code distribution information, and the unknown code satisfies the classification minimum requirement of the known code.Type: GrantFiled: July 23, 2018Date of Patent: November 2, 2021Assignee: Toshiba Memory CorporationInventor: Yukinobu Hayashida
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Patent number: 10711307Abstract: The invention relates to a method which is capable of determining, at an initial stage of differentiation, the differentiated state of undifferentiated stem cells. The invention provides a method for determining a differentiated state of a cell comprising detecting expression of FOXB2 gene of a stem cell, and determining the differentiated state based on the result, and a differentiation marker selected from mRNA or protein derived from the FOXB2 gene. The invention is applicable to quality management of the stem cells or to methods for preparation and isolation of the differentiated cells. Further, because the differentiated cells can be determined at an initial stage of culture, the invention is useful for an early stage screening of cells and for quality management of the stem cells, and reduction in culturing period and cost reduction in expenditure regarding a culture medium, or the like, can be expected.Type: GrantFiled: August 19, 2015Date of Patent: July 14, 2020Assignee: FUJIFILM WAKO PURE CHEMICAL CORPORATIONInventor: Yukinobu Hayashida
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Publication number: 20190285686Abstract: According to one embodiment, an arithmetic processor generates unknown code distribution information, for an unknown test code to which no failure mode has been specified, and determines whether labeling of the unknown code distribution information is performable. The arithmetic processor determines whether the unknown code distribution information agrees to known code distribution information, when labeling of the unknown code distribution information is performable. The arithmetic processor determines whether the unknown code satisfies a classification minimum requirement of the agreed known code. The arithmetic processor presents a content of EFA measurement that assumes a failure mode corresponding to the known code, when the unknown code distribution information agrees to the known code distribution information, and the unknown code satisfies the classification minimum requirement of the known code.Type: ApplicationFiled: July 23, 2018Publication date: September 19, 2019Applicant: Toshiba Memory CorporationInventor: Yukinobu HAYASHIDA
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Publication number: 20190226031Abstract: An object of the present invention is to provide a method capable of conveniently carrying out amplification of methylated DNA, and determination of methylation of DNA and determination of the occurrence of cancer using the amplification of methylated DNA. The present invention relates to “a method for amplifying a methylated analysis target region; a method for determining the methylation of an analysis target region; a reagent for use in the determination of methylation; a method for determining the occurrence of cancer; a method for obtaining data for determining the occurrence of cancer; a reagent for use in the determination of the occurrence of cancer; and a marker for use in the determination of the occurrence of cancer”.Type: ApplicationFiled: September 1, 2017Publication date: July 25, 2019Inventor: Yukinobu HAYASHIDA
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Publication number: 20170342490Abstract: The invention relates to a method which is capable of determining, at an initial stage of differentiation, the differentiated state of undifferentiated stem cells. The invention provides a method for determining a differentiated state of a cell comprising detecting expression of FOXB2 gene of a stem cell, and determining the differentiated state based on the result, and a differentiation marker selected from mRNA or protein derived from the FOXB2 gene. The invention is applicable to quality management of the stem cells or to methods for preparation and isolation of the differentiated cells. Further, because the differentiated cells can be determined at an initial stage of culture, the invention is useful for an early stage screening of cells and for quality management of the stem cells, and reduction in culturing period and cost reduction in expenditure regarding a culture medium, or the like, can be expected.Type: ApplicationFiled: August 19, 2015Publication date: November 30, 2017Applicant: WAKO PURE CHEMICAL INDUSTRIES, LTD.Inventor: Yukinobu HAYASHIDA
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Publication number: 20170321247Abstract: The invention relates to a cancer marker, consisting of a portion of or all of the nucleotide sequence represented by SEQ ID NO: 1 in a FOXB2 gene derived from a cell, and being continuous nucleotide sequences comprising at least the nucleotide sequence represented by SEQ ID NO: 2 or 3, wherein 30% or more of cytosines of the -CG- sequences present in said nucleotide sequence are methylated, and method for determining canceration of a cell on the basis of methylation ratio, wherein said methylation ratio is measured by a bisulfate reaction for a portion of or all of cytosines of the -CG- sequences present in the nucleotide sequence represented by SEQ ID NO: 1 in a FOXB2 gene derived from a cell.Type: ApplicationFiled: August 18, 2015Publication date: November 9, 2017Applicant: WAKO PURE CHEMICAL INDUSTRIES, LTD.Inventor: Yukinobu HAYASHIDA
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Patent number: 9624535Abstract: The invention provides a method for converting non-methylated cytosine in a single-stranded DNA into uracil by a bisulfite reaction with a high conversion efficiency from non-methylated cytosine into uracil. The invention also provides a method for amplifying the single-stranded DNA in which non-methylated cytosine has been converted into uracil, as well as a method for detecting methylated cytosine in the single-stranded DNA.Type: GrantFiled: December 10, 2012Date of Patent: April 18, 2017Assignee: Wako Pure Chemical Industries, Ltd.Inventors: Yukinobu Hayashida, Naoyuki Yamamoto
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Publication number: 20150307930Abstract: The present invention is to provide a method for detecting a hydroxymethylated cytosine in DNA and a detection kit therefor. The present invention relates to “a method for detecting the hydroxymethylated cytosine in DNA, which the method comprises: (1) a step in which a single-stranded DNA is contacted with (i) a polyvalent metal oxide or a polyvalent metal acid salt of a metal atom selected from group 6, group 8, group 9 and group 10 of the periodic table, and contacted with (ii) a peroxide selected from persulfuric acid, percarboxylic acids and the salts thereof; (2) a step in which a specific region of the single-stranded DNA treated in (1) is subjected to amplification treatment; (3) a step in which the presence or absence of an objective amplification product obtained in (2) is detected; and (4) a step in which on the basis of the results of (3), the presence or absence of hydroxymethylated cytosine in the specific region of DNA is determined.Type: ApplicationFiled: December 24, 2013Publication date: October 29, 2015Inventors: Yukinobu HAYASHIDA, Naoyuki YAMAMOTO
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Publication number: 20150176069Abstract: The invention provides a method for converting non-methylated cytosine in a single-stranded DNA into uracil by a bisulfite reaction with a high conversion efficiency from non-methylated cytosine into uracil. The invention also provides a method for amplifying the single-stranded DNA in which non-methylated cytosine has been converted into uracil, as well as a method for detecting methylated cytosine in the single-stranded DNA.Type: ApplicationFiled: December 10, 2012Publication date: June 25, 2015Inventors: Yukinobu Hayashida, Naoyuki Yamamoto
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Publication number: 20130337451Abstract: The invention provides a method for obtaining DNA after bisulfite reaction which can be stored with libraries in genome reserved and has excellent storage stability, and a method for detecting methylated cytosine. Specifically, the invention provides a method for obtaining DNA complementary to single-stranded DNA in which non-methylated cytosine has been uracilated by subjecting single-stranded DNA to a bisulfite reaction and then a reverse transcriptase reaction. The resulting complementary DNA can be amplified by a PCR reaction. Methylated cytosine can be detected in single-stranded DNA by subjecting the single-stranded DNA, in order, to a bisulfite reaction, a reverse transcriptase reaction, and a PCR reaction, and then subjecting the obtained PCR amplification product to nucleotide sequence analysis.Type: ApplicationFiled: December 14, 2011Publication date: December 19, 2013Applicant: WAKO PURE CHEMICAL INDUSTRIES, LTD.Inventor: Yukinobu Hayashida
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Publication number: 20120058520Abstract: An object of the present invention is to provide an inexpensive and simple method for synthesis of a double-stranded DNA corresponding to a particular RNA, and a method for amplification of the aforementioned double-stranded DNA.Type: ApplicationFiled: May 11, 2010Publication date: March 8, 2012Applicant: WAKO PURE CHEMICAL INDUSTRIES, LTD.Inventor: Yukinobu Hayashida
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Publication number: 20100209972Abstract: An object of the present invention is to provide a simple and safe method for synthesis of single-stranded or double-stranded DNA and a kit for performing the synthesis.Type: ApplicationFiled: October 1, 2008Publication date: August 19, 2010Applicant: WAKO PURE CHEMICAL INDUSTRIES, LTD.Inventor: Yukinobu Hayashida
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Patent number: 7749707Abstract: The present invention provides a method for obtaining or amplifying a polynucleotide (a tester-specific polynucleotide), in which an amount existing in a sample (tester) is larger than the amount existing in another sample (driver), easily and within a short time as well as with high efficiency, a polynucleotide obtained (amplified) by such method, a method for identifying gene mutation in the tester, and a kit to be used in such methods.Type: GrantFiled: February 27, 2006Date of Patent: July 6, 2010Assignee: Wako Pure Chemical Industries, Ltd.Inventor: Yukinobu Hayashida
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Publication number: 20090053698Abstract: The present invention provides a method for obtaining or amplifying a polynucleotide (a tester-specific polynucleotide), in which an amount existing in a sample (tester) is larger than the amount existing in another sample (driver), easily and within a short time as well as with high efficiency, a polynucleotide obtained (amplified) by such method, a method for identifying gene mutation in the tester, and a kit to be used in such methods.Type: ApplicationFiled: February 27, 2006Publication date: February 26, 2009Applicant: WAKO PURE CHEMICAL INDUSTRIES, LTD.Inventor: Yukinobu Hayashida
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Patent number: 6753240Abstract: The present invention provides a semiconductor device production method that eliminates the risk of the occurrence of residual resist in the production process, and as a result, allows the electrical characteristics and reliability of the device to be improved. In this semiconductor device production method comprising steps of: subsequently laminating a first resist layer and a second resist layer having desired patterns on a semiconductor substrate, forming a first conductive region on the semiconductor substrate by injecting a first ion into the semiconductor substrate using the first and second resist layers as masks, removing the second resist layer, forming a second conductive region on the semiconductor substrate by injecting a second ion into the semiconductor substrate using the remaining first resist layer as a mask, and removing the first resist layer.Type: GrantFiled: May 21, 2002Date of Patent: June 22, 2004Assignee: UMC JapanInventor: Yukinobu Hayashida
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Publication number: 20030045076Abstract: The present invention provides a semiconductor device production method that eliminates the risk of the occurrence of residual resist in the production process, and as a result, allows the electrical characteristics and reliability of the device to be improved. In this semiconductor device production method comprising steps of: subsequently laminating a first resist layer and a second resist layer having desired patterns on a semiconductor substrate, forming a first conductive region on the semiconductor substrate by injecting a first ion into the semiconductor substrate using the first and second resist layers as masks, removing the second resist layer, forming a second conductive region on the semiconductor substrate by injecting a second ion into the semiconductor substrate using the remaining first resist layer as a mask, and removing the first resist layer.Type: ApplicationFiled: May 21, 2002Publication date: March 6, 2003Applicant: UMC JapanInventor: Yukinobu Hayashida