Patents by Inventor Yukoh Hiei
Yukoh Hiei has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230113805Abstract: The present invention relates to a nucleic acid molecule encoding an RNA-guided DNA endonuclease, which is (a) a nucleic acid molecule encoding the RNA-guided DNA endonuclease comprising or consisting of the amino acid sequence of SEQ ID NO: 1 or 3; (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of SEQ ID NO: 2 or 4; (c) a nucleic acid molecule encoding a RNA-guided DNA endonuclease the amino acid sequence of which is at least 70% identical to the amino acid sequence of (a); preferably at least 80% identical, more preferably at least 90% identical, and most preferred at least 95% identical; (d) a nucleic acid molecule comprising or consisting of a nucleotide sequence which is at least 70% identical to the nucleotide sequence of (b), preferably at least 80% identical, more preferably at least 90% identical, and most preferred at least 95% identical; (e) a nucleic acid molecule which is degenerate with respect to the nucleic acid molecule of (d); or (f) a nucleic acid molecule cType: ApplicationFiled: March 11, 2021Publication date: April 13, 2023Applicant: BRAIN BIOTECH AGInventors: Paul SCHOLZ, Uemit PUL, Christian ZUREK, Yukoh HIEI, Chizu YANAGIHARA, Hiroshi TSUKAMOTO, Toshihiko KOMARI
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Patent number: 11136587Abstract: The present invention relates to a method of obtaining a transformed plant cell. The present invention comprises the steps of: (a) co-transforming an intended DNA and a first marker gene into a plant cell; and (b) selecting from the transformed cells obtained in the step (a), a transformed plant cell wherein the intended DNA is introduced into a chromosome thereof, and the first marker gene is not introduced, wherein the method does not contain a step to exclude a transformed cell with only the intended DNA introduced into the chromosome by positive selection using the first marker gene.Type: GrantFiled: February 4, 2015Date of Patent: October 5, 2021Assignee: KANEKA CORPORATIONInventors: Yukoh Hiei, Toshihiko Komari
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Patent number: 10266835Abstract: This invention relates to the Agrobacterium bacterium to be used in plant transformation method comprising three types of plasmids. The Agrobacterium bacterium of this invention comprises plasmids of (1) to (3) shown below: (1) a plasmid comprising the following components: (i) the virB gene, the virC gene, the virD1 gene, the virD2 gene, the virD3 gene, the virG gene and the virJ gene of pTiBo542, and (ii) an origin of replication; (2) a disarmed Ti plasmid or a disarmed Ri plasmid of Agrobacterium bacterium; and (3) a plasmid having a T-DNA region consisting of a desired DNA; wherein each of the plasmids of (1) to (3) has a replication mechanism that enables a mutual coexistence with each other.Type: GrantFiled: March 27, 2014Date of Patent: April 23, 2019Assignee: JAPAN TOBACCO INC.Inventors: Teruyuki Imayama, Yukoh Hiei, Yuji Ishida
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Patent number: 9840714Abstract: A method for gene introduction by which higher efficiency for gene introduction than that by the conventional Agrobacterium method may be attained simply and without injuring the tissue is disclosed. According to the method of the present invention, the efficiency of gene introduction into plant cells by a bacterium belonging to genus Agrobacterium is promoted by accompanying centrifugation of the plant cells or plant tissue.Type: GrantFiled: May 3, 2011Date of Patent: December 12, 2017Assignee: JAPAN TOBACCO INC.Inventors: Yukoh Hiei, Keisuke Kasaoka, Yuji Ishida
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Patent number: 9556450Abstract: Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a DTP21 polypeptide.Type: GrantFiled: December 15, 2014Date of Patent: January 31, 2017Assignees: E I DU PONT DE NEMOURS AND COMPANY, JAPAN TOBACCO INC, PIONEER HI-BRED INTL INC.Inventors: Marc C Albertsen, Yukoh Hiei, Teruyuki Imayama, Masakazu Kashihara, Norio Kato, Toshihiko Komari, Toshiyuki Komori, Stanley Luck, Ichiro Oka, Hajime Sakai, Yoshimitsu Takakura, Scott V Tingey, Satoru Usami
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Publication number: 20160340682Abstract: The present invention relates to a method of obtaining a transformed plant cell. The present invention comprises the steps of: (a) co-transforming an intended DNA and a first marker gene into a plant cell; and (b) selecting from the transformed cells obtained in the step (a), a transformed plant cell wherein the intended DNA is introduced into a chromosome thereof, and the first marker gene is not introduced, wherein the method does not contain a step to exclude a transformed cell with only the intended DNA introduced into the chromosome by positive selection using the first marker gene.Type: ApplicationFiled: February 4, 2015Publication date: November 24, 2016Applicant: JAPAN TOBACCO INC.Inventors: Yukoh HIEI, Toshihiko KOMARI
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Publication number: 20160083737Abstract: This invention relates to the Agrobacterium bacterium to be used in plant transformation method comprising three types of plasmids. The Agrobacterium bacterium of this invention comprises plasmids of (1) to (3) shown below: (1) a plasmid comprising the following components: (i) the virB gene, the virC gene, the virD1 gene, the virD2 gene, the virD3 gene, the virG gene and the virJ gene of pTiBo542, and (ii) an origin of replication; (2) a disarmed Ti plasmid or a disarmed Ri plasmid of Agrobacterium bacterium; and (3) a plasmid having a T-DNA region consisting of a desired DNA; wherein each of the plasmids of (1) to (3) has a replication mechanism that enables a mutual coexistence with each other.Type: ApplicationFiled: March 27, 2014Publication date: March 24, 2016Applicant: JAPAN TOBACCO INC.Inventors: Teruyuki IMAYAMA, Yukoh HIEI, Yuji ISHIDA
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Patent number: 9284567Abstract: An object of the present invention is to provide a method of gene introduction, which can transform a Hordeum plant at a higher efficiency compared to that in known Agrobacterium methods, and a method of producing a transformed Hordeum plant. The method of the invention includes a step of subjecting an immature embryo tissue of a Hordeum plant to centrifugation treatment and/or pressurization treatment before the inoculation with Agrobacterium, during the coculture step, and/or after the coculture step, and is characterized in that the coculture medium satisfies at least one of a) containing an antiauxin; b) containing a cytokinin; and c) containing a phenoxy auxin in an amount of less than 2 ?M and/or a benzoic auxin in an amount of less than 5 ?M, or not containing any phenoxy auxin and/or benzoic auxin.Type: GrantFiled: July 29, 2011Date of Patent: March 15, 2016Assignee: JAPAN TOBACCO INC.Inventors: Yukoh Hiei, Yuji Ishida
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Publication number: 20150101078Abstract: Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a DTP21 polypeptide.Type: ApplicationFiled: December 15, 2014Publication date: April 9, 2015Inventors: MARC C ALBERTSEN, Yukoh Hiei, Teruyuki Imayama, Masakazu Kashihara, Norio Kato, Toshihiko Komari, Toshiyuki Komori, Stanley Luck, Ichiro Oka, Hajime Sakai, Yoshimitsu Takakura, Scott V Tingey, Satoru Usami
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Patent number: 8916746Abstract: Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a DTP21 polypeptide.Type: GrantFiled: October 29, 2010Date of Patent: December 23, 2014Assignees: Japan Tobacco, Inc., E. I. du Pont de Neumours and Company, Pioneer Hi-Bred International, Inc.Inventors: Masakazu Kashihara, Toshiyuki Komori, Ichiro Oka, Satoru Usami, Norio Kato, Yukoh Hiei, Yoshimitsu Takakura, Toshihiko Komari, Teruyuki Imayama, Scott V. Tingey, Hajime Sakai, Marc C. Albertsen, Stanley Luck
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Patent number: 8884101Abstract: The method of the present invention includes the step of excising one or more portions selected from a radicle, a germ, and an embryonic axis of a plant tissue inoculated with Agrobacterium after cultivation in a coculture medium. The present invention provides a method of gene introduction that can transform a Triticum plant at high efficiency compared to conventionally known Agrobacterium methods, and provides a method of producing a transformed plant.Type: GrantFiled: July 29, 2010Date of Patent: November 11, 2014Assignee: Japan Tobacco Inc.Inventors: Yuji Ishida, Yukoh Hiei
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Publication number: 20130125266Abstract: An object of the present invention is to provide a method of gene introduction, which can transform a Hordeum plant at a higher efficiency compared to that in known Agrobacterium methods, and a method of producing a transformed Hordeum plant. The method of the invention includes a step of subjecting an immature embryo tissue of a Hordeum plant to centrifugation treatment and/or pressurization treatment before the inoculation with Agrobacterium, during the coculture step, and/or after the coculture step, and is characterized in that the coculture medium satisfies at least one of a) containing an antiauxin; b) containing a cytokinin; and c) containing a phenoxy auxin in an amount of less than 2 ?M and/or a benzoic auxin in an amount of less than 5 ?M, or not containing any phenoxy auxin and/or benzoic auxin.Type: ApplicationFiled: July 29, 2011Publication date: May 16, 2013Applicant: JAPAN TOBBACCO INC.Inventors: Yukoh Hiei, Yuji Ishida
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Patent number: 8357836Abstract: An Agrobacterium-mediated method for producing transformed maize or rice, culturing an Agrobacterium-inoculated immature embryo with a coculture medium and a regeneration step for culturing the immature embryo with a regeneration medium either without callus growth or after callus growth culture to regenerate whole transformed maize or rice. The method further includes a transformation enhancement and the method does not include any selection step based on the properties of a nucleic acid to be introduced by Agrobacterium in any step from coculture to regeneration.Type: GrantFiled: March 24, 2009Date of Patent: January 22, 2013Assignee: Japan Tobacco Inc.Inventors: Yuji Ishida, Yukoh Hiei
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Patent number: 8324456Abstract: The present invention provides a method for Agrobacterium-mediated gene transfer into a plant material, which comprises inoculating an Agrobacterium into the plant material in the presence of a powder. In the method of the present invention, the powder at least does not affect living tissues and has one or more properties selected from the group consisting of: being insoluble in water; having an affinity for living tissues; having adsorption properties; and having a surface polarity. The present invention also provides a method for producing a transformed plant, which comprises using the gene transfer method of the present invention.Type: GrantFiled: December 13, 2006Date of Patent: December 4, 2012Assignee: Japan Tobacco Inc.Inventors: Yuji Ishida, Yukoh Hiei, Jun Ueki, Takeshi Yamamoto
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Patent number: 8298819Abstract: The present invention aims to provide novel vectors for plant transformation. The vectors of the present invention are cosmid vectors having a full length of 15 kb or less characterized in that: 1) they contain an origin of replication of an IncP plasmid, but do not contain any origin of replication of other plasmid groups; 2) they contain the trfA1 gene of an IncP plasmid; 3) they contain an oriT of an IncP plasmid; 4) they contain the incC1 gene of an IncP plasmid; 5) they contain a cos site of lambda phage and the cos site is located outside the T-DNA; 6) they contain a drug resistance gene expressed in E.Type: GrantFiled: June 25, 2007Date of Patent: October 30, 2012Assignee: Japan Tobacco Inc.Inventors: Yoshimitsu Takakura, Toshihiko Komari, Yuji Ishida, Toshiyuki Komori, Yukoh Hiei, Toshiki Mine, Teruyuki Imayama
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Publication number: 20120124696Abstract: The method of the present invention includes the step of excising one or more portions selected from a radicle, a germ, and an embryonic axis of a plant tissue inoculated with Agrobacterium after cultivation in a coculture medium. The present invention provides a method of gene introduction that can transform a Triticum plant at high efficiency compared to conventionally known Agrobacterium methods, and provides a method of producing a transformed plant.Type: ApplicationFiled: July 29, 2010Publication date: May 17, 2012Inventors: Yuji Ishida, Yukoh Hiei
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Patent number: 8101820Abstract: The present invention aims to provide a method for increasing transformation efficiency in plants when compared to conventionally known Agrobacterium-mediated methods. In the present invention, one of the features is to comprise a coculture step for culturing an Agrobacterium-inoculated plant tissue with a coculture medium containing 3,6-dichloro-o-anisic acid.Type: GrantFiled: February 28, 2008Date of Patent: January 24, 2012Assignee: Japan Tobacco Inc.Inventors: Yuji Ishida, Yukoh Hiei
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Patent number: 8058514Abstract: A novel Agrobacterium-mediated method for producing a transformed monocotyledonous plant is provided. The transformation method involves (i) a coculture step for culturing an Agrobacterium-inoculated monocotyledonous plant tissue with a coculture medium containing 3,6-dichloro-o-anisic acid, 4-amino-3,5,6-trichloropicolinic acid and/or 2,4,5-trichlorophenoxyacetic acid, and (ii) a regeneration step for culturing the tissue obtained in (i) with a regeneration medium containing a selective drug to thereby induce regeneration and to produce a transformed plant. The method does not include, between the coculture step and the regeneration step, any selection step for culturing the cocultured tissue with a medium containing an auxin and a selective drug to select a transformant.Type: GrantFiled: February 28, 2008Date of Patent: November 15, 2011Assignee: Japan Tobacco Inc.Inventors: Yuji Ishida, Yukoh Hiei
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Publication number: 20110277181Abstract: Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a DTP21 polypeptide.Type: ApplicationFiled: October 29, 2010Publication date: November 10, 2011Applicants: EI DU PONT DE NEMOURS AND COMPANY, JAPAN TOBACCO INC, PIONEER HI-BRED INTERNATIONAL INCInventors: MASAKAZU KASHIHARA, TOSHIYUKI KOMORI, ICHIRO OKA, SATORU USAMI, NORIO KATO, YUKOH HIEI, YOSHIMITSU TAKAKURA, TOSHIHIKO KOMARI, TERUYUKI IMAYAMA, SCOTT V. TINGEY, HAJIME SAKAI, MARC C. ALBERTSEN, STANLEY LUCK
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Patent number: 8039687Abstract: The present invention provides a method for selecting genomic DNA fragments which are useful for providing a plant with an agriculturally advantageous improvement. The method of the present invention comprises the steps of: 1) preparing genomic DNA from a plant, which is then cloned into a cloning vector to form a genomic DNA library; 2) introducing a genomic fragment from each of the genomic clones constituting the genomic DNA library separately into a plant to produce transgenic plants; 3) cultivating the transgenic plants or progeny thereof to select a plant exhibiting an agriculturally advantageous phenotypic variation; and 4) selecting the genomic DNA fragment, which was introduced in step (2) into the plant selected in step (3), as a purposed genomic DNA fragment.Type: GrantFiled: October 22, 2004Date of Patent: October 18, 2011Assignee: Japan Tobacco Inc.Inventors: Tomoaki Kubo, Toshihiko Komari, Satoru Usami, Yoshimitsu Takakura, Yukoh Hiei, Yuji Ishida