Abstract: The present invention provides a biosensor, wherein non-specific adsorption on a surface that does not retain a physiologically active substance (reference unit) is suppressed. The biosensor of the invention includes a substrate having at least two types of surfaces on a single plane, wherein at least one of the surfaces does not retain a physiologically active substance, and wherein at least two types of hydrophilic compounds with different molecular weights are bound to the surface that does not retain a physiologically active substance.

Abstract: It is an object of the present invention to provide a method for producing a solid substrate used for sensors having a film with a small film thickness distribution, and a solid surface used for sensors having a film with a small film thickness distribution. The present invention provides a method for producing a solid substrate for sensors that has a coating on the surface using spin coating, wherein a substrate to be coated is rotated in an atmosphere in which the vapor pressure of coating solvent is 50% to 100% with respect to the saturation vapor pressure so as to form a thin film of a coating solution on said substrate to be coated.

Abstract: It is an object of the present invention to provide a biosensor on which a large amount of protein can be immobilized and nonspecific adsorption is less likely to occur with the use of a SAM compound having high water solubility and good performance in terms of supply. The present invention provides a biosensor comprising a substrate on which a hydrophilic polymer having a reactive group capable of binding to a physiologically active substance is immobilized via a compound represented by the following formula (1), said compound at a concentration of 1 mM being dissolved in water at 25° C.: X-L-Y??(1) wherein X represents a group capable of binding to metal, L represents a linking group, and Y represents a functional group to which the hydrophilic polymer is bound.

Abstract: It is an object of the present invention to provide a biosensor on which a large amount of protein can be immobilized and nonspecific adsorption is less likely to occur with the use of a SAM compound having high water solubility and good performance in terms of supply. The present invention provides a biosensor comprising a substrate on which a hydrophilic polymer having a reactive group capable of binding to a physiologically active substance is immobilized via a compound represented by the following formula (1), said compound at a concentration of 1 mM being dissolved in water at 25° C.: X-L-Y??(1) wherein X represents a group capable of binding to metal, L represents a linking group, and Y represents a functional group to which the hydrophilic polymer is bound.

Abstract: It is an object of the present invention to provide a method for producing a solid substrate used for sensors having a film with a small film thickness distribution, and a solid surface used for sensors having a film with a small film thickness distribution. The present invention provides a method for producing a solid substrate for sensors that has a coating on the surface using spin coating, wherein a substrate to be coated is rotated in an atmosphere in which the vapor pressure of coating solvent is 50% to 100% with respect to the saturation vapor pressure so as to form a thin film of a coating solution on said substrate to be coated.

Abstract: It is an object of the present invention to provide a substrate for sensors and a container, wherein the adhesiveness of the substrate to a thin film is improved, a physiologically active substance can be immobilized without the peeling of the thin film from a plastic substrate, and non-specific adsorption during the analysis of interaction among biomolecules is small. The present invention provides a substrate for sensors, which has a thin film layer on a plastic substrate, wherein the plastic substrate has been treated with an organic primer before formation of the thin film.

Abstract: It is an object of the present invention to provide a biosensor, wherein non-specific adsorption on a surface that does not retain a physiologically active substance (reference unit) is suppressed. The present invention provides a biosensor which comprises a substrate having at least two types of surfaces on a single plane, wherein at least one of said surfaces does not retain a physiologically active substance, and wherein at least two types of hydrophilic compounds with different molecular weights are bound to said surface that does not retain a physiologically active substance.

Abstract: It is an object of the present invention to provide a method for producing a solid substrate used for sensors having a film with a small film thickness distribution, and a solid surface used for sensors having a film with a small film thickness distribution. The present invention provides a method for spin coating wherein the substrate is rotated in a state where the substrate surface to be coated is inclined against the rotation surface during coating.

Abstract: It is an object of the present invention to provide a solid substrate used for sensors that suppresses nonspecific adsorption and that is able to immobilize a physiologically active substance. The present invention provides A solid substrate used for sensors, wherein two or more different hydrophobic polymer layers are laminated on the solid substrate, and among the above hydrophobic polymer layers, the surface of a layer, which is farthest from the solid substrate, is modified.