Abstract: It is an object of the present invention to provide a physiologically active substance-immobilized substrate wherein the stability of the physiologically active substance has been improved by adding a compound having the effect of suppressing deactivation to the substrate on which the physiologically active substance has been immobilized. The present invention provides a substrate which has, on the surface thereof, a physiologically active substance that has been immobilized thereon via a hydrophilic polymer layer formed with hydrophilic polymers, and Compound having a mean molecular weight between 350 and 5,000,000 and having a residue capable of forming a hydrogen bond.

Abstract: It is an object of the present invention to provide a biosensor substrate that prevents non-specific adsorption and is used for immobilizing a physiologically active substance. The present invention provides a biosensor substrate which comprises an inorganic oxide film and a hydrophilic polymer having a physiologically active substance-immobilizing group on the substrate, wherein the hydrophilic polymer is bound to the inorganic oxide film through a low molecular compound.

Abstract: An object of the present invention is to provide a method for production of a physiologically active substance-immobilized substrate, which can prevent the inactivation of a physiologically active substance and/or can immobilize a physiologically active substance at a high concentration onto a substrate surface without use of electrostatic attraction. The present invention provides a method for production of a physiologically active substance-immobilized substrate, which comprises steps of: applying, to a metal substrate having a layer for immobilizing a physiologically active substance, a solution containing a physiologically active substance capable of forming a covalent bond with a molecule constituting the layer for immobilizing a physiologically active substance; and then drying the solution.