Patents by Inventor Yulei MA

Yulei MA has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240124853
    Abstract: Provided are transaminase mutants and uses thereof. The transaminase mutant is obtained by one or more amino acid mutations occurring in SEQ ID NO: 2 or is a mutant with a conserved amino acid mutation obtained by taking the sequence SEQ ID NO: 1 of a wild-type CvTA transaminase as a reference. Compared with wild-type transaminases, the catalytic activity of the mutant is improved to different degrees, so that the production efficiency of chiral amine compound synthesis may be improved.
    Type: Application
    Filed: July 6, 2021
    Publication date: April 18, 2024
    Inventors: Hao Hong, Gage James, Yi Xiao, Na Zhang, Xuecheng Jiao, Yulei Ma, Huiyan Mou, Zujian Wang, Kaihua Sun, Xiang Li, Tong Zhao, Shan Cao
  • Publication number: 20240117395
    Abstract: Provided is a method for synthesizing a chiral amine compound. A transaminase is used to transaminate a ketone substrate under the action of an amino donor, to obtain the chiral amine compound; and the conserved amino acid sequence region of the transaminase at least includes a region 1 (MAGLWCVN) and a region 2 (YNTFFKT). With the transaminase with the specific conserved amino acid sequence region to synthesize a large sterically hindered chiral amine, the enzyme catalytic reaction volume is small, the synthesizing route is short, the product yield is high, a high-cost noble metal is not required for catalysis under the synthesizing conditions, three wastes are reduced, and the production cost is saved.
    Type: Application
    Filed: July 6, 2021
    Publication date: April 11, 2024
    Inventors: Hao Hong, Gage James, Yi Xiao, Na Zhang, Xuecheng Jiao, Yulei Ma, Huiyan Mou, Zujian Wang, Kaihua Sun, Ru Jia, Fang Liu, Wenjing Liu
  • Patent number: 11952575
    Abstract: A transaminase mutant and use hereof, the amino acid sequence of the transaminase mutant is an amino acid sequence in which the amino acid sequence as represented by SEQ ID NO: 1 is mutated, the mutated amino acid position being one or more selected from among F89, K193, P243, V234, I262, Q280, V379, R416, A417 and C418. The enzymatic activity and/or stability of the transaminase mutant is improved.
    Type: Grant
    Filed: November 15, 2017
    Date of Patent: April 9, 2024
    Assignee: ASYMCHEM LIFE SCIENCE (TIANJIN) CO., LTD
    Inventors: Hao Hong, Gage James, Jiangping Lu, Xingfu Xu, Wenyan Yu, Xin Huang, Yulei Ma, Yibing Cheng
  • Publication number: 20240010997
    Abstract: Provided are a cytochrome P450 enzyme mutant and an application thereof. The enzyme activity and anti-Markov oxidation selectivity of P450 derived from a wild-type strain of Bacillus megaterium undergo protein modification by means of directed evolution, thus improving enzyme activity and selectivity, and developing a series of P450 enzyme mutants that may be used for industrial production.
    Type: Application
    Filed: April 25, 2021
    Publication date: January 11, 2024
    Inventors: Hao HONG, Gage JAMES, Na ZHANG, Xuecheng JIAO, Yulei MA, Shan CAO, Yibing CHENG, Kaihua SUN
  • Patent number: 11827909
    Abstract: Provided are a proline hydroxylase and uses thereof. The proline hydroxylase comprises having the amino acid sequence of SEQ ID NO: 2 with the exception of a mutation of one or more amino acids; wherein the mutation of one or more amino acids must comprises E27K, and the mutation of one or more amino acids selected from the group consisting of: H14R, L16N, T25R, F26L, E27K, D30S, S33N, E34N, E34G, E34L, E34S, E34D, Y35W, Y35K, S37W, S37F, S37E, S37N, S37T, S37C, W40F, K41E, D54G, H55Q, S57L, I58T, I58Y, I58A, I58R, I58V, I58S, I58C, K86P, T91A, F95Y, C97Y, I98V, K106V, K106T, K106Q, F111S, K112E, K112R, S154A, K162E, L166M, I118F, I118V, I118R, H119R, H119F, I120V, K123D, K123N, K123Q, K123S, K123I, K123T, T130N, D134G, V135K, N165H, D173G, K209R, I223V and S225A, and having proline hydroxylase activity.
    Type: Grant
    Filed: December 10, 2020
    Date of Patent: November 28, 2023
    Assignees: ASYMCHEM LABORATORIES (TIANJIN) CO., LTD., ASYMCHEM LIFE SCIENCE (TIANJIN) CO., LTD., TIANJIN ASYMCHEM PHARMACEUTICALS CO., LTD., ASYMCHEM LABORATORIES (FUXIN) CO., LTD., JILIN ASYMCHEM LABORATORIES CO., LTD.
    Inventors: Hao Hong, Gage James, Jiangping Lu, Na Zhang, Wenyan Yu, Fang Liu, Yanjun Li, Xin Huang, Juan Gao, Kejian Zhang, Yulei Ma, Junlu Wei
  • Publication number: 20230235300
    Abstract: Provided are a transaminase mutant and an application thereof. Compared with an amino acid sequence shown in SEQ ID NO:1, an amino acid sequence of the transaminase mutant includes at least one of the following mutation sites: L166, K149, K146, A168, H73, F133, H82, E24, V194, T294, A295, G235 and F236. The mutant of the present invention has the improved catalytic activity for a transammonization reaction of ketone substrates, and is suitable for industrial production of chiral amines.
    Type: Application
    Filed: May 29, 2020
    Publication date: July 27, 2023
    Inventors: Hao HONG, Gage JAMES, Yi XIAO, Na ZHANG, Xuecheng JIAO, Yulei MA, Huiyan MU, Shan CAO
  • Publication number: 20230227797
    Abstract: Provided are a transaminase mutant and use thereof. The transaminase mutant has an amino acid sequence obtained by mutation of an amino acid sequence shown in SEQ ID NO:1, the mutation at least includes one of the following mutation site combinations: T7C+S47C, Q78C+A330C, V137C+G313C, A217C+Y252C and L295C+C328C; or the transaminase mutant has an amino acid sequence which has the mutation sites in the mutated amino acid sequence and has 80% or more identity with the mutated amino acid sequence. The transaminase mutant realizes the change of protein structure and functions, reduces the enzyme amount, increases the enantiomeric excess (ee) value of a product, and reduces the difficulty of post-processing, so that the transaminase mutant may be suitable for industrial production.
    Type: Application
    Filed: October 28, 2019
    Publication date: July 20, 2023
    Inventors: Hao HONG, Gage JAMES, Yi XIAO, Yulei MA, Na ZHANG, Xuecheng JIAO, Huiyan MU, Yibing CHENG, Shan CAO
  • Patent number: 11359219
    Abstract: The invention provides a transaminase mutant and application thereof, wherein the amino acid sequence of the transaminase mutant is formed after mutation of the amino acid sequence as shown in SEQ ID NO: 1, and mutated amino acid sites comprise T7C+S47C sites. The transaminase mutant having the mutation sites can be further prepared into an immobilized enzyme through an immobilization technology, the immobilized enzyme has relatively high activity and high stability, can be recycled for multiple times, and is applicable to continuous flow reaction in a packed bed.
    Type: Grant
    Filed: February 5, 2018
    Date of Patent: June 14, 2022
    Assignee: ASYMCHEM LIFE SCIENCE (TIANJIN) CO., LTD
    Inventors: Hao Hong, Gage James, Jiangping Lu, Xingfu Xu, Yuxia Cui, Na Zhang, Xuewu Dong, Wenyan Yu, Xin Huang, Mingmin Hao, Yulei Ma, Yibing Cheng, Jiadong Zhao
  • Publication number: 20220145341
    Abstract: Disclosed are a monooxygenase mutant and use thereof. An amino acid sequence of a monooxygenase mutant is an amino acid sequence obtained by mutating an amino acid sequence as shown in SEQ ID NO: 1. Mutation includes at least one of the following mutation sites: site 49, site 60, site 61, site 144, site 145, site 146, site 147, site 167, site 169, site 189, site 246, site 247, site 280, site 284, site 285, site 286, site 287, site 328, site 330, site 332, site 382, site 427, site 428, site 429, site 430, site 431, site 432, site 433, site 434, site 435, site 436, site 438, site 441, site 493, site 494, site 508, site 509, site 510, site 511, site 512, and site 513 sites and the like. The monooxygenase mutant has the advantage of greatly improved stereoselectivity, and the enzyme activity is also improved correspondingly.
    Type: Application
    Filed: April 17, 2019
    Publication date: May 12, 2022
    Inventors: Hao Hong, Gage James, Jiangping Lu, Na Zhang, Yulei Ma, Yibing Cheng, Huiyan Mu
  • Patent number: 11162081
    Abstract: A ketoreductase mutant and use thereof are provided. The amino acid sequence of the ketoreductase mutant is an amino acid sequence obtained by mutation of the amino acid sequence shown in SEQ ID NO: 1, wherein the mutation at least comprises one of the following mutation sites: position 6, position 94, position 96, position 117, position 144, position 156, position 193, position 205, position 224, position 176, position 85 and position 108; alternatively, the amino acid sequence of the ketoreductase mutant has a mutation site in a mutated amino acid sequence and an amino acid sequence having 80% or more homology with the mutated amino acid sequence.
    Type: Grant
    Filed: January 22, 2018
    Date of Patent: November 2, 2021
    Assignee: JILIN ASYMCHEM LABORATORIES CO., LTD.
    Inventors: Hao Hong, Gage James, Jiangping Lu, Xingfu Xu, Wenyan Yu, Na Zhang, Yulei Ma, Yibing Cheng, Huiyan Mu
  • Publication number: 20210095262
    Abstract: Provided are a proline hydroxylase and uses thereof. The proline hydroxylase comprises having the amino acid sequence of SEQ ID NO: 2 with the exception of a mutation of one or more amino acids; wherein the mutation of one or more amino acids must comprises E27K, and the mutation of one or more amino acids selected from the group consisting of: H14R, L16N, T25R, F26L, E27K, D30S, S33N, E34N, E34G, E34L, E34S, E34D, Y35W, Y35K, S37W, S37F, S37E, S37N, S37T, S37C, W40F, K41E, D54G, H55Q, S57L, I58T, I58Y, I58A, I58R, I58V, I58S, I58C, K86P, T91A, F95Y, C97Y, I98V, K106V, K106T, K106Q, F111S, K112E, K112R, S154A, K162E, L166M, I118F, I118V, I118R, H119R, H119F, I120V, K123D, K123N, K123Q, K123S, K123I, K123T, T130N, D134G, V135K, N165H, D173G, K209R, I223V and S225A, and having proline hydroxylase activity.
    Type: Application
    Filed: December 10, 2020
    Publication date: April 1, 2021
    Inventors: Hao HONG, Gage JAMES, Jiangping LU, Na ZHANG, Wenyan YU, Fang LIU, Yanjun LI, Xin HUANG, Juan GAO, Kejian ZHANG, Yulei MA, Junlu WEI
  • Patent number: 10961516
    Abstract: Provided are a proline hydroxylase and uses thereof. The proline hydroxylase comprises (a) a protein having the amino acid sequence as shown in SEQ ID NO: 2; (b) a protein having an amino acid sequence of SEQ HD NO: 2 with a mutation of one or more amino acids and having a proline hydroxylase activity; or (c) a protein retaining the mutation of one or more amino acids as in (b), and having the proline hydroxylase activity and having at least 78% homology with the amino acid sequence of the protein in (b). Protein having the amino acid sequence as shown in SEQ HD NO: 2 and mutants obtained by genetically engineering have higher catalytic specificity or significantly increased catalytic activity when compared to proline hydroxylases in prior art.
    Type: Grant
    Filed: November 4, 2016
    Date of Patent: March 30, 2021
    Assignees: ASYMCHEM LABORATORIES (TIANJIN) CO., LTD., ASYMCHEM LIFE SCIENCES (TIANJIN) CO., LTD., TIANJIN ASYMCHEM PHARMACEUTICALS CO., LTD., ASYMCHEM LABORATORIES (FUXIN) CO., LTD., JILIN ASYMCHEM LABORATORIES CO., LTD.
    Inventors: Hao Hong, Gage James, Jiangping Lu, Na Zhang, Wenyan Yu, Fang Liu, Yanjun Li, Xin Huang, Juan Gao, Kejian Zhang, Yulei Ma, Junlu Wei
  • Publication number: 20210054428
    Abstract: The invention provides a transaminase mutant and application thereof, wherein the amino acid sequence of the transaminase mutant is formed after mutation of the amino acid sequence as shown in SEQ ID NO: 1, and mutated amino acid sites comprise T7C+S47C sites. The transaminase mutant having the mutation sites can be further prepared into an immobilized enzyme through an immobilization technology, the immobilized enzyme has relatively high activity and high stability, can be recycled for multiple times, and is applicable to continuous flow reaction in a packed bed.
    Type: Application
    Filed: February 5, 2018
    Publication date: February 25, 2021
    Inventors: Hao HONG, Gage JAMES, Jiangping LU, Xingfu XU, Yuxia CUI, Na ZHANG, Xuewu DONG, Wenyan YU, Xin HUANG, Mingmin HAO, Yulei MA, Yibing CHENG, Jiadong ZHAO
  • Publication number: 20200354694
    Abstract: A ketoreductase mutant and use thereof are provided. The amino acid sequence of the ketoreductase mutant is an amino acid sequence obtained by mutation of the amino acid sequence shown in SEQ ID NO: 1, wherein the mutation at least comprises one of the following mutation sites: position 6, position 94, position 96, position 117, position 144, position 156, position 193, position 205, position 224, position 176, position 85 and position 108; alternatively, the amino acid sequence of the ketoreductase mutant has a mutation site in a mutated amino acid sequence and an amino acid sequence having 80% or more homology with the mutated amino acid sequence.
    Type: Application
    Filed: January 22, 2018
    Publication date: November 12, 2020
    Inventors: Hao HONG, Gage JAMES, Jiangping LU, Xingfu XU, Wenyan YU, Na ZHANG, Yulei MA, Yibing CHENG, Huiyan MU
  • Publication number: 20200239895
    Abstract: A transaminase mutant and use hereof, the amino acid sequence of the transaminase mutant is an amino acid sequence in which the amino acid sequence as represented by SEQ ID NO: 1 is mutated, the mutated amino acid position being one or more selected from among F89, K193, P243, V234, I262, Q280, V379, R416, A417 and C418. The enzymatic activity and/or stability of the transaminase mutant is improved.
    Type: Application
    Filed: November 15, 2017
    Publication date: July 30, 2020
    Applicant: Asymchem Life Science (Tianjin) Co., Ltd
    Inventors: Hao HONG, Gage JAMES, Jiangping LU, Xingfu XU, Wenyan YU, Xin HUANG, Yulei MA, Yibing CHENG
  • Publication number: 20200199544
    Abstract: Provided are a proline hydroxylase and uses thereof. The proline hydroxylase comprises (a) a protein having the amino acid sequence as shown in SEQ ID NO: 2; (b) a protein having an amino acid sequence of SEQ HD NO: 2 with a mutation of one or more amino acids and having a proline hydroxylase activity; or (c) a protein retaining the mutation of one or more amino acids as in (b), and having the proline hydroxylase activity and having at least 78% homology with the amino acid sequence of the protein in (b). Protein having the amino acid sequence as shown in SEQ HD NO: 2 and mutants obtained by genetically engineering have higher catalytic specificity or significantly increased catalytic activity when compared to proline hydroxylases in prior art.
    Type: Application
    Filed: November 4, 2016
    Publication date: June 25, 2020
    Inventors: Hao HONG, Gage JAMES, Jiangping LU, Na ZHANG, Wenyan YU, Fang LIU, Yanjun LI, Xin HUANG, Juan GAO, Kejian ZHANG, Yulei MA, Junlu WEI