Abstract: A reading-line adjusting device of an image scanner includes an image sensing module, a shaft, a bush member, and a fixing and adjusting member. The image sensing module has a reading line for sensing an electronic signal of an image. The shaft is arranged in a first direction. The bush member includes a base and a bushing body. The base is coupled with the image sensing module. The bushing body is sleeved around the shaft and moved along the shaft. The fixing and adjusting member is used for fixing the base of the bush member onto the image sensing module and adjusting an angle between the base and the image sensing module such that the reading line of the image sensing module is arranged in a second direction.