Abstract: The present invention relates to a kit of in vitro quantifying large surface protein of hepatitis B virus (LHBS). The kit includes monoclonal antibodies having respective binding specificity for specific regions of LHBS, thereby increasing sensitivity and dynamic breadth of detecting LHBS in a biological sample. Moreover, the invention also provides a biomarker set corresponding to the specific regions of LHBS, and the biomarker set can be specifically recognized by the monoclonal antibodies, for non-invasively analyzing phases of HBV infection and hepatoma prognosis in a biological sample. Furthermore, the invention also provides a set of monoclonal antibodies for predicting, diagnosing or treating a chronic liver disease via those biomarkers in a subject in need thereof.

Abstract: A buffer member includes a first buffer portion, a pin, a bending portion and a second buffer portion. The pin is located on the first buffer portion. The bending portion is connected with the first buffer portion. The second buffer portion is connected with the bending portion, and the first buffer portion is connected with the second buffer portion via the bending portion. A reinforcing member is located inside the pin for raising the rigidity of the pin. The pin is used for fixing a vibration source. The first buffer portion and the second buffer portion reduce the transmission of the vibration energy from the vibration source.

Abstract: A buffer member includes a first buffer portion, a pin, a bending portion and a second buffer portion. The pin is located on the first buffer portion. The bending portion is connected with the first buffer portion. The second buffer portion is connected with the bending portion, and the first buffer portion is connected with the second buffer portion via the bending portion. A reinforcing member is located inside the pin for raising the rigidity of the pin. The pin is used for fixing a vibration source. The first buffer portion and the second buffer portion reduce the transmission of the vibration energy from the vibration source.

Abstract: A buffer member includes a first buffer portion, a pin, a bending portion and a second buffer portion. The pin is located on the first buffer portion. The bending portion is connected with the first buffer portion. The second buffer portion is connected with the bending portion, and the first buffer portion is connected with the second buffer portion via the bending portion. A reinforcing member is located inside the pin for raising the rigidity of the pin. The pin is used for fixing a vibration source. The first buffer portion and the second buffer portion reduce the transmission of the vibration energy from the vibration source.

Abstract: A method of detecting pre-S2 deletion mutant LHBS is disclosed herein. The method comprises incubating a biological sample with a first antibody to captured HBS proteins; detecting the LHBS and WT LHBS bound to the immobilized first antibody, respectively; and calculating the amount of the pre-S2 deletion mutant LHBS protein by subtracting the amount of the WT LHBS protein from that of the LHBS protein. Advantageously, by the method described herein, the amount of the pre-S2 deletion mutant LHBS, a potential high-risk marker for HCC incidence in chronic HBV carriers and recurrence in HCC patients after hepatectomy surgery, in a biological sample may be easily calculated without mutual influence between the WT and pre-S mutant LHBS while reducing the labor-intensive process for cloning each gene product before analysis.

Abstract: A HBS-specific antibody, a LHBS-specific antibody, a WT LHBS-specific antibody, an immunoassay kit comprising the antibodies, and a method of detecting pre-S2 deletion mutant LHBS using the immunoassay kit are disclosed herein. The method comprises incubating a biological sample with a first antibody to captured HBS proteins; detecting the LHBS and WT LHBS bound to the immobilized first antibody, respectively; and calculating the amount of the pre-S2 deletion mutant LHBS protein by subtracting the amount of the WT LHBS protein from that of the LHBS protein. Advantageously, by the method described herein, the amount of the pre-S2 deletion mutant LHBS, a potential high-risk marker for HCC incidence in chronic HBV carriers and recurrence in HCC patients after hepatectomy surgery, in a biological sample may be easily calculated without mutual influence between the WT and pre-S mutant LHBS while reducing the labor-intensive process for cloning each gene product before analysis.

Abstract: A buffer member includes a first buffer portion, a pin, a bending portion and a second buffer portion. The pin is located on the first buffer portion. The bending portion is connected with the first buffer portion. The second buffer portion is connected with the bending portion, and the first buffer portion is connected with the second buffer portion via the bending portion. A reinforcing member is located inside the pin for raising the rigidity of the pin. The pin is used for fixing a vibration source. The first buffer portion and the second buffer portion reduce the transmission of the vibration energy from the vibration source.

Abstract: A HBS-specific antibody, a LHBS-specific antibody, a WT LHBS-specific antibody, an immunoassay kit comprising the antibodies, and a method of detecting pre-S2 deletion mutant LHBS using the immunoassay kit are disclosed herein. The method comprises incubating a biological sample with a first antibody to captured HBS proteins; detecting the LHBS and WT LHBS bound to the immobilized first antibody, respectively; and calculating the amount of the pre-S2 deletion mutant LHBS protein by subtracting the amount of the WT LHBS protein from that of the LHBS protein. Advantageously, by the method described herein, the amount of the pre-S2 deletion mutant LHBS, a potential high-risk marker for HCC incidence in chronic HBV carriers and recurrence in HCC patients after hepatectomy surgery, in a biological sample may be easily calculated without mutual influence between the WT and pre-S mutant LHBS while reducing the labor-intensive process for cloning each gene product before analysis.

Abstract: A spherical pivot bearing assembly structure and a holder using the same are provided. The spherical pivot bearing assembly structure comprises a spherical pivot, a bearing housing, a friction member, an elastic member and a fixing member, wherein the elastic member disposed between the friction member and the elastic member applies an elastic force on the friction member to force the friction member tightly against a spherical surface of the spherical pivot and uniformly distribute the loading applied on the friction member so as to prevent slippage of the friction member. The elastic member may be a spring shaped in coils, wherein the amount of coils can be adjusted as being applied to different types of electronic apparatuses in different dimensions.