Abstract: A magnetic field measurement device and a magnetic field measurement method based on solid-state spins are provided. Zeeman splitting between electron energy levels of electrons of a magnetic sensitive unit is different under the action of external magnetic fields having different intensities. Continuous excitation and manipulation is applied to the electrons by means of a control field and control laser, so that spins of a system can reach a balanced state. The spin population of the system is influenced by the external magnetic fields. Therefore, fluorescence signals with intensities changing with the spin population of the system can be obtained, and fluorescent signals having different intensities can be obtained according to the different intensities of the external magnetic fields.

Abstract: A magnetic field measurement device and a magnetic field measurement method based on solid-state spins are provided. Zeeman splitting between electron energy levels of electrons of a magnetic sensitive unit is different under the action of external magnetic fields having different intensities. Continuous excitation and manipulation is applied to the electrons by means of a control field and control laser, so that spins of a system can reach a balanced state. The spin population of the system is influenced by the external magnetic fields. Therefore, fluorescence signals with intensities changing with the spin population of the system can be obtained, and fluorescent signals having different intensities can be obtained according to the different intensities of the external magnetic fields.

Abstract: A method is provided for purifying a recombinant protein from a mixture comprising the recombinant protein and related proteins, comprising the steps of: A. using a first equilibrating buffer in a first conductivity and pH to make the recombinant protein bind to an ion exchange medium; B. using a second equilibration buffer in a second conductivity and pH to continually equilibrate the ion exchange medium bound to the protein; C. using a washing liquid in a third conductivity and a gradually increasing pH to wash the ion-exchange medium, and eluting the first category-related proteins; D. using a first eluent in a fourth conductivity and pH to elute the ion exchange medium, and eluting the target recombinant protein; and E. using a second eluent in a fifth conductivity and pH to continually elute the ion exchange medium, and eluting the second category-related proteins.

Abstract: A method is provided for purifying a recombinant protein from a mixture comprising the recombinant protein and related proteins, comprising the steps of: A. using a first equilibrating buffer in a first conductivity and pH to make the recombinant protein bind to an ion exchange medium; B. using a second equilibration buffer in a second conductivity and pH to continually equilibrate the ion exchange medium bound to the protein; C. using a washing liquid in a third conductivity and a gradually increasing pH to wash the ion-exchange medium, and eluting the first category-related proteins; D. using a first eluent in a fourth conductivity and pH to elute the ion exchange medium, and eluting the target recombinant protein; and E. using a second eluent in a fifth conductivity and pH to continually elute the ion exchange medium, and eluting the second category-related proteins.