Abstract: Disclosed is a complex tFNA-miR22 for treating optic nerve disease, which complex is composed of tetrahedral DNA and miR-22 according to a molar ratio of 1:(1-4). The tFNA-miR22 can effectively inhibit apoptosis of retinal ganglion cells and promote the release of a brain-derived neurotrophic factor (BDNF), thereby achieving a good protection effect for the retinal ganglion cells. The tFNA-miR22 is used for preparing optic nerve protection drugs, the treatment of neurodegenerative optic nerve diseases including glaucoma is facilitated, and the tFNA-miR22 has very good application prospects.

Abstract: A neural network construction method and system in the field of artificial intelligence, to construct a target neural network by replacing a part of basic units in an initial backbone network with placeholder modules, so that different target neural networks can be constructed based on different scenarios. The method may include obtaining an initial backbone network and a candidate set, replacing at least one basic unit in the initial backbone network with at least one placeholder module to obtain a to-be-determined network, performing sampling based on the candidate set to obtain information about at least one sampling structure, and obtaining a network model based on the to-be-determined network and the information about the at least one sampling structure. The information about the at least one sampling structure may be used for determining a structure of the at least one placeholder module.

Abstract: This invention discloses a nucleic acid aptamer AS1411 modified DNA tetrahedron. The preparation method includes the steps of (1) binding an AS1411 sequence to the 5? terminal of any DNA single-strand in a DNA tetrahedron, synthesizing the DNA, dissolving obtained DNA powder with ddH2O; (2) measuring an absorbance of the DNA and then calculating a total volume of the four single strands; (3) pipetting the DNA obtained in step (1) according to the calculation results in step (2), mixing the DNA with a TM buffer, mixing the mixture uniformly with vortex vibration, and performing a PCR progress. The preparation method is simple. The produced product can effectively solve the problem that the unmodified DNA tetrahedron cannot enter the nucleus and the problem that the AS1411 cannot carry drugs directly.

Abstract: This invention discloses a method for preparing TDNs-AS1411-nucleic acid drug nanocomposite based drug delivery system, which includes the following steps: binding AS1411 and nucleic acid drug to a tetrahedral DNA nanostructure respectively; selecting four DNA single strands that respectively carry AS1411 and nucleic acid drug; mixing the four DNA single strands; mixing the DNA single strands and the TM buffer uniformly; putting the mixture into a PCR apparatus; raising the temperature to 95° C. quickly and maintaining for 10 min; and next cooling down to 4° C. and maintaining for 20 min to obtain the TDNs-AS1411-nucleic acid drug nanocomposite based drug delivery system. This drug delivery system can directly act on cell nucleus and will not be degraded by lysosomal. The targeting specificity is good. The drug can take a good efficacy and the pertinency is high.

Abstract: This invention discloses a nucleic acid aptamer AS1411 modified DNA tetrahedron. The preparation method includes the steps of (1) binding an AS1411 sequence to the 5? terminal of any DNA single-strand in a DNA tetrahedron, synthesizing the DNA, dissolving obtained DNA powder with ddH2O; (2) measuring an absorbance of the DNA and then calculating a total volume of the four single strands; (3) pipetting the DNA obtained in step (1) according to the calculation results in step (2), mixing the DNA with a TM buffer, mixing the mixture uniformly with vortex vibration, and performing a PCR progress. The preparation method is simple. The produced product can effectively solve the problem that the unmodified DNA tetrahedron cannot enter the nucleus and the problem that the AS1411 cannot carry drugs directly.

Abstract: This invention discloses a method for preparing TDNs-AS1411-nucleic acid drug nanocomposite based drug delivery system, which includes the following steps: binding AS1411 and nucleic acid drug to a tetrahedral DNA nanostructure respectively; selecting four DNA single strands that respectively carry AS1411 and nucleic acid drug; mixing the four DNA single strands; mixing the DNA single strands and the TM buffer uniformly; putting the mixture into a PCR apparatus; raising the temperature to 95° C. quickly and maintaining for 10 min; and next cooling down to 4° C. and maintaining for 20 min to obtain the TDNs-AS1411-nucleic acid drug nanocomposite based drug delivery system. This drug delivery system can directly act on cell nucleus and will not be degraded by lysosomal. The targeting specificity is good. The drug can take a good efficacy and the pertinency is high.

Abstract: Generation, coding and transmission of an effective video form, scalable portrait video. As an expansion to bi-level video, portrait video is composed of more gray levels, and therefore possesses higher visual quality while it maintains a low bit rate and low computational costs. Portrait video is a scalable video in that each video with a higher level always contains all the information of the video with a lower level. The bandwidths of 2-4 level portrait videos fit into the bandwidth range of 20-40 Kbps that GPRS and CDMA 1X can stably provide. Therefore, portrait video is very promising for video broadcast and communication on 2.5 G wireless networks. With portrait video technology, this system and method is the first to enable two-way video conferencing on Pocket PCs and Handheld PCs.

Abstract: Generation, coding and transmission of an effective video form, scalable portrait video. As an expansion to bi-level video, portrait video is composed of more gray levels, and therefore possesses higher visual quality while it maintains a low bit rate and low computational costs. Portrait video is a scalable video in that each video with a higher level always contains all the information of the video with a lower level. The bandwidths of 2-4 level portrait videos fit into the bandwidth range of 20-40 Kbps that GPRS and CDMA 1X can stably provide. Therefore, portrait video is very promising for video broadcast and communication on 2.5 G wireless networks. With portrait video technology, this system and method is the first to enable two-way video conferencing on Pocket PCs and Handheld PCs.

Abstract: Generation, coding and transmission of an effective video form, scalable portrait video. As an expansion to bi-level video, portrait video is composed of more gray levels, and therefore possesses higher visual quality while it maintains a low bit rate and low computational costs. Portrait video is a scalable video in that each video with a higher level always contains all the information of the video with a lower level. The bandwidths of 2–4 level portrait videos fit into the bandwidth range of 20–40 Kbps that GPRS and CDMA 1X can stably provide. Therefore, portrait video is very promising for video broadcast and communication on 2.5 G wireless networks. With portrait video technology, this system and method is the first to enable two-way video conferencing on Pocket PCs and Handheld PCs.

Abstract: Generation, coding and transmission of an effective video form, scalable portrait video. As an expansion to bi-level video, portrait video is composed of more gray levels, and therefore possesses higher visual quality while it maintains a low bit rate and low computational costs. Portrait video is a scalable video in that each video with a higher level always contains all the information of the video with a lower level. The bandwidths of 2-4 level portrait videos fit into the bandwidth range of 20-40 Kbps that GPRS and CDMA 1× can stably provide. Therefore, portrait video is very promising for video broadcast and communication on 2.5G wireless networks. With portrait video technology, this system and method is the first to enable two-way video conferencing on Pocket PCs and Handheld PCs.

Abstract: Generation, coding and transmission of an effective video form, scalable portrait video. As an expansion to bi-level video, portrait video is composed of more gray levels, and therefore possesses higher visual quality while it maintains a low bit rate and low computational costs. Portrait video is a scalable video in that each video with a higher level always contains all the information of the video with a lower level. The bandwidths of 2-4 level portrait videos fit into the bandwidth range of 20-40 Kbps that GPRS and CDMA 1X can stably provide. Therefore, portrait video is very promising for video broadcast and communication on 2.5G wireless networks. With portrait video technology, this system and method is the first to enable two-way video conferencing on Pocket PCs and Handheld PCs.