Abstract: A pupil tracking method includes: retrieving an external eye image of a subject, wherein the external eye image includes a pupil of the subject; performing an image preprocessing on the external eye image, wherein the image preprocessing includes performing a binary conversion on the external eye image to obtain a binary image; finding out a contour boundary of each feature in the binary image, and finding out a pupil feature based on a variance of a distance from the contour boundary of each feature to a corresponding reference point; fitting the contour boundary of the pupil feature by a boundary fitting method to find a center coordinate of the pupil feature. The abovementioned pupil tracking method can track the pupil of the subject's eyeball without using a stereo camera. An ophthalmology inspection device using the abovementioned pupil tracking method is also disclosed.

Abstract: A fundus camera displays guide information on a display panel which can be viewed by a testee to guide the testee to adjust a relative position of the fundus camera and a tested eyeball of the testee to a determined position. Therefore, the testee can use the above-mentioned fundus camera to self-shoot fundus images with better image quality. A method for self-shooting fundus is also disclosed.

Abstract: A fundus camera displays guide information on a display panel which can be viewed by a testee to guide the testee to adjust a relative position of the fundus camera and a tested eyeball of the testee to a determined position. Therefore, the testee can use the above-mentioned fundus camera to self-shoot fundus images with better image quality. A method for self-shooting fundus is also disclosed.

Abstract: Disclosed is a transfection method, which includes the steps of: (a) adhering the gene fragments to dry ice particles; (b) adding the dry ice particles into the medium/liquid that contains target cells; and (c) transporting the gene fragments into the target cells via the micro explosion/sublimation of the dry ice particles. In addition, the gene fragments can also adhere first to nanoparticles, which can then adhere to dry ice particles. Subsequently, gene fragments enter cells by micro explosion/sublimation. The present invention can be applied in transgenic research on prokaryotic, eukaryotic, plant and animal cells and in the development of new species in agriculture.

Abstract: The present invention relates to a retro-reflector microarray including a plurality of micro retro-reflectors arranged on a common plane. Each of the retro-reflectors is a concave corner cube consisting of three mutually orthogonal reflective surfaces. The concave corner cubes are the main reflecting elements of the microarray and make the reflected light anti-parallel to the incident light. The retro-reflector microarray can be used in optical detection instrument as an auxiliary element for remotely scanning fluorescence and Raman signals.

Abstract: Disclosed is a transfection method, which includes the steps of: (a) adhering the gene fragments to dry ice particles; (b) adding the dry ice particles into the medium/liquid that contains target cells; and (c) transporting the gene fragments into the target cells via the micro explosion/sublimation of the dry ice particles. In addition, the gene fragments can also adhere first to nanoparticles, which can then adhere to dry ice particles. Subsequently, gene fragments enter cells by micro explosion/sublimation. The present invention can be applied in transgenic research on prokaryotic, eukaryotic, plant and animal cells and in the development of new species in agriculture.