Abstract: The present invention discloses a high-entropy soft magnetic alloy with 900 K high-temperature resistance, comprising Fe, Co, Ni, Si and Al, and the atomic percent of the alloy composition is expressed as FexCoyNizSimAln, wherein x=40%-80%, y=20%-60%, z=0-30%, m=0-20%, n=0-20%, and x+y+z+m+n=100%; the atomic percent of other doping elements is p=0-5%, and 0.5?m/n?3; the performance indexes of the material include: at room temperature, saturation magnetization Ms=90-150 emu/g, and coercive force Hc=0.1-15 Oe; and at 900 K, saturation magnetization Ms=70-130 emu/g, and coercive force Hc=0.1-25 Oe.

Abstract: A two-photon stimulated emission depletion composite microscope using continuous light loss, the microscope comprising: a two-photon imaging unit (100) and an STED imaging unit (200), wherein for thicker samples, the two-photon imaging unit (100) can be used, and for regions of interest on the surface of samples, the STED super-resolution imaging unit (200) can be used. The two-photon stimulated emission depletion composite microscope using continuous light loss integrates two functions of STED imaging and two-photon imaging, so as to provide a powerful tool for cutting-edge biomedical research.

Abstract: A two-photon stimulated emission depletion composite microscope, comprising a two-photon imaging unit (100) and an STED imaging unit (200), wherein the two-photon imaging unit (100) can be used for a relatively thick sample, and the STED super-resolution imaging unit can be used for a region of interest on a surface of a sample, and the microscope makes light spots generated by an excitation light and a depletion light after being focused by an objective lens (OL) accurately coincide in a three-dimensional distribution. The two-photon stimulated emission depletion composite microscope (10) integrates two functions of STED imaging and two-photon imaging and makes the two types of light spots generated by an excitation light and a depletion light after being focused by an objective lens accurately coincide in a three-dimensional distribution, thereby providing a powerful tool for cutting-edge biomedical research.

Abstract: A STED super-resolution microscope capable of quick beam combination is disclosed, which includes a STED imaging unit and a beam combination test unit. The excitation light and the depletion light are accurately combined by the beam combination test unit, so that the imaging light spots of the two light beams passing through the STED imaging unit can accurately coincide with each other, thereby obtaining a better super-resolution imaging effect.

Abstract: A two-photon stimulated emission depletion composite microscope, comprising a two-photon imaging unit (100) and an STED imaging unit (200), wherein the two-photon imaging unit (100) can be used for a relatively thick sample, and the STED super-resolution imaging unit can be used for a region of interest on a surface of a sample, and the microscope makes light spots generated by an excitation light and a depletion light after being focused by an objective lens (OL) accurately coincide in a three-dimensional distribution. The two-photon stimulated emission depletion composite microscope (10) integrates two functions of STED imaging and two-photon imaging and makes the two types of light spots generated by an excitation light and a depletion light after being focused by an objective lens accurately coincide in a three-dimensional distribution, thereby providing a powerful tool for cutting-edge biomedical research.

Abstract: A two-photon stimulated emission depletion composite microscope using continuous light loss, the microscope comprising: a two-photon imaging unit (100) and an STED imaging unit (200), wherein for thicker samples, the two-photon imaging unit (100) can be used, and for regions of interest on the surface of samples, the STED super-resolution imaging unit (200) can be used. The two-photon stimulated emission depletion composite microscope using continuous light loss integrates two functions of STED imaging and two-photon imaging, so as to provide a powerful tool for cutting-edge biomedical research.

Abstract: Disclosed is a STED super-resolution microscope capable of quick beam combination, which includes a STED imaging unit and a beam combination test unit. The excitation light and the depletion light are accurately combined by the beam combination test unit, so that the imaging light spots of the two light beams passing through the STED imaging unit can accurately coincide with each other, thereby obtaining a better super-resolution imaging effect.

Abstract: Disclosed are a low-fluorescence-photobleaching confocal imaging method and system. A confocal image is first selected as a reference image, and a threshold is set based on pixel values of the reference image. Then the density of fluorescent molecules in a pixel is determined based on a result of comparison of a real-time fluorescence intensity feedback and the threshold. Finally, an illumination time for the pixel is controlled based on the density of fluorescent molecules in the pixel to obtain a low-fluorescence-photobleaching confocal imaging image. The low-fluorescence-photobleaching confocal imaging method and system provided herein control the illumination time for each object-side pixel to make more efficient use of fluorescence information and reduce fluorescence photobleaching without sacrificing image quality, and so can be applied to a variety of biological samples. Further provided is a low-fluorescence-photobleaching confocal imaging system.