Abstract: The present invention provides a simple and rapid construction of an animal model of constipation and use thereof. The construction method comprises: irritating a rat via tail-clamping and then gavaging the rat with 3.5-7 mg/kg of loperamide the next day, wherein the tail-clamping irritation treatment is as follows: clamping a tail 2-4 times every day for 25-35 min each time continuously for 3-5 days. The present invention obtains a method for constructing a rat model of constipation caused by liver depression and spleen deficiency by combining the tail-clamping irritation treatment and loperamide gavage treatment. The method has a short modeling period, is simple to operate, has a low cost, and effectively overcomes stress reaction of a rat. Besides, the constructed rat model of constipation caused by liver depression and spleen deficiency has various mechanisms, being stable, reliable and high in repeatability.