Patents by Inventor YunWei Charles Cao
YunWei Charles Cao has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11273433Abstract: The disclosure relates to a single-atom-based catalyst system with total-length control of single-atom catalytic sites. The single-atom-based catalyst system comprises at least one catalyst structure comprising a first assembly of a plurality of single-atom-catalyst superparticles. The single-atom-catalyst superparticles comprise a second assembly of a plurality of single-atom-catalyst nanoparticles. The single-atom-based catalyst system has controlled porosity and spatial distribution of active single-atom catalysts from the atomic scale to the macroscopic scale. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.Type: GrantFiled: February 17, 2021Date of Patent: March 15, 2022Assignee: University of Florida Research Foundation, Inc.Inventor: Yunwei Charles Cao
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Publication number: 20210187489Abstract: The disclosure relates to a single-atom-based catalyst system with total-length control of single-atom catalytic sites. The single-atom-based catalyst system comprises at least one catalyst structure comprising a first assembly of a plurality of single-atom-catalyst superparticles. The single-atom-catalyst superparticles comprise a second assembly of a plurality of single-atom-catalyst nanoparticles. The single-atom-based catalyst system has controlled porosity and spatial distribution of active single-atom catalysts from the atomic scale to the macroscopic scale. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.Type: ApplicationFiled: February 17, 2021Publication date: June 24, 2021Inventor: Yunwei Charles CAO
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Publication number: 20210139873Abstract: Disclosed herein are improved nanozymes for targeting RNA. The disclosed nanozymes are synthesized using recombinant RNase A with site-specific cysteine-substituted mutations that can be covalently functionalized with a length-tunable multi-thiol tether and then loaded onto gold particles through multiple gold-sulfur bonds. This new RNase A loading mechanism is site specific, and it allows high-density loading of alkylthiol modified DNA oligonucleotides. The disclosed nanozymes can also include additional capturer strands and/or involve DNA-recombinant-RNase-A unibodies to further increase the nanozyme's enzymatic activity and target selectivity. Also disclosed are functional on-off switchable nanozymes to control nanozyme activity. In some embodiments, the disclosed nanozyme are core-free hollow forms.Type: ApplicationFiled: November 6, 2020Publication date: May 13, 2021Inventors: Yunwei Charles Cao, Tian Jiang
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Patent number: 10968474Abstract: Described herein are fluid-manipulation-based devices and methods of use. Fluid manipulations according to devices and methods as described herein can be configured to perform assays on biological samples. Devices and methods as described herein can manipulate and analyze nanoliter volumes of fluid, microliter volumes of fluid, milliliter volumes of fluid, or greater. Embodiments of the present disclosure can enable random biological assays and rapid, simultaneous analysis of multiple biological samples.Type: GrantFiled: March 27, 2019Date of Patent: April 6, 2021Assignee: University of Florida Research Foundation Inc.Inventor: Yunwei Charles Cao
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Patent number: 10538757Abstract: Embodiments of the present disclosure provide for nanozymes that can include a therapeutic agent, methods of making nanozymes, methods of using nanozymes, and the like. In some embodiments, the nanozymes can include a shell that can surround a hollow core that can be configured to receive a compound and the shell can include a recognition moiety and an enzyme.Type: GrantFiled: February 12, 2016Date of Patent: January 21, 2020Assignee: University of Florida Research Foundation, Inc.Inventors: Yunwei Charles Cao, Chen Liu
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Publication number: 20190323069Abstract: Described herein are fluid-manipulation-based devices. Fluid manipulations as described herein can be configured to perform assays on biological samples. In an embodiment, the device includes a reaction chamber, which can include an integrated sample isolation module, a cell lysis module, a biological target purification module, and an assay mixing module, which can include a microbead with a capture molecule coupled thereto and a nanoparticle having a probe molecule coupled thereto via a label, which can be a spectroscopic label. In an embodiment, the capture and probe molecules can be configured to be coupled together via a biological target to form a biological molecule bead complex. Devices and methods as described herein can manipulate and analyze nanoliter volumes of fluid, microliter volumes of fluid, milliliter volumes of fluid, or greater. Embodiments of the present disclosure can enable random biological assays and rapid, simultaneous analysis of multiple biological samples.Type: ApplicationFiled: March 27, 2019Publication date: October 24, 2019Inventor: Yunwei Charles Cao
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Patent number: 10301667Abstract: Described herein are fluid-manipulation-based devices. Fluid manipulations as described herein can be configured to perform assays on biological samples. In an embodiment, the device includes a reaction chamber, which can includes an integrated sample isolation module, a cell lysis module, a biological target purification module, and an assay mixing module, which can include a microbead with a capture molecule coupled thereto and a nanoparticle having a probe molecule coupled thereto via a label, which can be a spectroscopic label. In an embodiment, the capture and probe molecules can be configured to be coupled together via a biological target to form a biological molecule bead complex. Devices and methods as described herein can manipulate and analyze nanoliter volumes of fluid, microliter volumes of fluid, milliliter volumes of fluid, or greater. Embodiments of the present disclosure can enable random biological assays and rapid, simultaneous analysis of multiple biological samples.Type: GrantFiled: November 18, 2016Date of Patent: May 28, 2019Assignee: University of Florida Research Foundation, Inc.Inventor: Yunwei Charles Cao
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Patent number: 10081542Abstract: Embodiments of the present disclosure provides for nanozymes, methods of making nanozymes, methods of using nanozymes, and the like.Type: GrantFiled: April 19, 2011Date of Patent: September 25, 2018Assignee: University of Florida Research Foundation, lnc.Inventors: Yunwei Charles Cao, Chen Liu, Hongyan Liu, Zhongliang Wang, Soon Hye Yang
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Publication number: 20170138941Abstract: Described herein are fluid-manipulation-based devices. Fluid manipulations as described herein can be configured to perform assays on biological samples. In an embodiment, the device includes a reaction chamber, which can includes an integrated sample isolation module, a cell lysis module, a biological target purification module, and an assay mixing module, which can include a microbead with a capture molecule coupled thereto and a nanoparticle having a probe molecule coupled thereto via a label, which can be a spectroscopic label. In an embodiment, the capture and probe molecules can be configured to be coupled together via a biological target to form a biological molecule bead complex. Devices and methods as described herein can manipulate and analyze nanoliter volumes of fluid, microliter volumes of fluid, milliliter volumes of fluid, or greater. Embodiments of the present disclosure can enable random biological assays and rapid, simultaneous analysis of multiple biological samples.Type: ApplicationFiled: November 18, 2016Publication date: May 18, 2017Inventor: Yunwei Charles Cao
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Publication number: 20160215279Abstract: Embodiments of the present disclosure provide for nanozymes that can include a therapeutic agent, methods of making nanozymes, methods of using nanozymes, and the like.Type: ApplicationFiled: February 12, 2016Publication date: July 28, 2016Inventors: Yunwei Charles Cao, Chen Liu
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Patent number: 9291758Abstract: Nanorods assemblies that have lengths in excess of 50 microns to meters are formed from contacting rice-shaped colloidal superparticles that are aligned along the long axis of the colloidal superparticles. The rice-shaped colloidal superparticles are formed from a multiplicity of nanorods with a high degree of association that is end to end to form colloidal superparticles that are in excess of three microns in length and have a length to diameter ratio of about three or more. Methods of preparing the rice-shaped colloidal superparticles employ mixing with an additional ligand to the nanorods to bias the self assembly of the nanorods by solvophobic interactions. Methods of preparing the nanorods assemblies include the infusion of the rice-shaped colloidal superparticles into microchannels patterned on a substrate, wherein the rice-shaped colloidal superparticles' long axes align in the microchannels.Type: GrantFiled: December 16, 2013Date of Patent: March 22, 2016Assignee: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.Inventors: Yunwei Charles Cao, Tie Wang, Xirui Wang
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Publication number: 20140319563Abstract: Nanorods assemblies that have lengths in excess of 50 microns to meters are formed from contacting rice-shaped colloidal superparticles that are aligned along the long axis of the colloidal superparticles. The rice-shaped colloidal superparticles are formed from a multiplicity of nanorods with a high degree of association that is end to end to form colloidal superparticles that are in excess of three microns in length and have a length to diameter ratio of about three or more. Methods of preparing the rice-shaped colloidal superparticles employ mixing with an additional ligand to the nanorods to bias the self assembly of the nanorods by solvophobic interactions. Methods of preparing the nanorods assemblies include the infusion of the rice-shaped colloidal superparticles into microchannels patterned on a substrate, wherein the rice-shaped colloidal superparticles' long axes align in the microchannels.Type: ApplicationFiled: December 16, 2013Publication date: October 30, 2014Applicant: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.Inventors: Yunwei Charles Cao, Tie Wang, Xirui Wang
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Publication number: 20130034532Abstract: Embodiments of the present disclosure provides for nanozymes, methods of making nanozymes, methods of using nanozymes, and the like.Type: ApplicationFiled: April 19, 2011Publication date: February 7, 2013Inventors: Yunwei Charles Cao, Chen Liu, Hongyan Liu, Zhongliang Wang, Soon Hye Yang
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Patent number: 8137457Abstract: A method of forming monodisperse metal chalcogenide nanocrystals without precursor injection, comprising the steps of: combining a metal source, a chalcogen oxide or a chalcogen oxide equivalent, and a fluid comprising a reducing agent in a reaction pot at a first temperature to form a liquid comprising assembly; increasing the temperature of the assembly to a sufficient-temperature to initiate nucleation to form a plurality of metal chalcogenide nanocrystals; and growing the plurality of metal chalcogenide nanocrystals without injection of either the metal source or the chalcogen oxide at a temperature equal to or greater than the sufficient-temperature, wherein crystal growth proceeds substantially without nucleation to form a plurality of monodisperse metal chalcogenide nanocrystals. Well controlled monodispersed CdSe nanocrystals of various sizes can be prepared by choice of the metal source and solvent system.Type: GrantFiled: October 10, 2008Date of Patent: March 20, 2012Assignee: University of Florida Research Foundation, Inc.Inventors: Yunwei Charles Cao, Ou Chen
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Patent number: 7754173Abstract: A method of forming rare earth oxide nanocrystals include the steps of dissolving a rare earth including compound in a solution containing at least one organic solvent, heating the solution to a temperature of at least 160° C., wherein a concentration of the rare earth including compound provided upon decomposition is sufficient to provide critical supersaturation of at least one active intermediate in the solution to nucleate a plurality of rare earth oxide nanocrystals. The plurality of rare earth nanocrystals are then grown, wherein the growing step proceeds at least in part in the absence of critical supersaturation of the active intermediate. The rare earth nanocrystals can assemble into at least one close-packed, ordered nanocrystal superlattice.Type: GrantFiled: March 20, 2009Date of Patent: July 13, 2010Assignee: University of Florida Research Foundation, Inc.Inventor: Yunwei Charles Cao
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Publication number: 20090196815Abstract: A method of forming rare earth oxide nanocrystals include the steps of dissolving a rare earth including compound in a solution containing at least one organic solvent, heating the solution to a temperature of at least 160° C., wherein a concentration of the rare earth including compound provided upon decomposition is sufficient to provide critical supersaturation of at least one active intermediate in the solution to nucleate a plurality of rare earth oxide nanocrystals. The plurality of rare earth nanocrystals are then grown, wherein the growing step proceeds at least in part in the absence of critical supersaturation of the active intermediate. The rare earth nanocrystals can assemble into at least one close-packed, ordered nanocrystal superlattice.Type: ApplicationFiled: March 20, 2009Publication date: August 6, 2009Applicant: University of Florida Research Foundation, Inc.Inventor: Yunwei Charles Cao
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Patent number: 7527773Abstract: A method of forming rare earth oxide nanocrystals include the steps of dissolving a rare earth including compound in a solution containing at least one organic solvent, heating the solution to a temperature of at least 160° C., wherein a concentration of the rare earth including compound provided upon decomposition is sufficient to provide critical supersaturation of at least one active intermediate in the solution to nucleate a plurality of rare earth oxide nanocrystals. The plurality of rare earth nanocrystals are then grown, wherein the growing step proceeds at least in part in the absence of critical supersaturation of the active intermediate. The rare earth nanocrystals can assemble into at least one close-packed, ordered nanocrystal superlattice.Type: GrantFiled: March 30, 2005Date of Patent: May 5, 2009Assignee: University of Florida Research Foundation, Inc.Inventor: Yunwei Charles Cao
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Publication number: 20090084307Abstract: A method of forming monodisperse metal chalcogenide nanocrystals without precursor injection, comprising the steps of: combining a metal source, a chalcogen oxide or a chalcogen oxide equivalent, and a fluid comprising a reducing agent in a reaction pot at a first temperature to form a liquid comprising assembly; increasing the temperature of the assembly to a sufficient-temperature to initiate nucleation to form a plurality of metal chalcogenide nanocrystals; and growing the plurality of metal chalcogenide nanocrystals without injection of either the metal source or the chalcogen oxide at a temperature equal to or greater than the sufficient-temperature, wherein crystal growth proceeds substantially without nucleation to form a plurality of monodisperse metal chalcogenide nanocrystals. Well controlled monodispersed CdSe nanocrystals of various sizes can be prepared by choice of the metal source and solvent system.Type: ApplicationFiled: October 10, 2008Publication date: April 2, 2009Applicant: University of Florida Research Foundation, Inc.Inventors: Yunwei Charles Cao, Ou Chen
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Patent number: 7465352Abstract: A method of homogeneously forming metal chalcogenide nanocrystals includes the steps combining a metal source, a chalcogenide source, and at least one solvent at a first temperature to form a liquid comprising assembly, and heating the assembly at a sufficient temperature to initiate nucleation to form a plurality of metal chalcogenide nanocrystals. The plurality of metal chalcogenide nanocrystals are then grown without injection of either the metal source or the chalcogenide source at a temperature at least equal to the sufficient temperature, wherein growth proceeds substantially without nucleation to form a plurality of monodisperse metal chalcogenide nanocrystals. An optional nucleation initiator can help control the final size of the monodisperse crystals. Such synthesis, without the need for precursor injection, is suitable for the industrial preparation of high-quality nanocrystals.Type: GrantFiled: July 25, 2005Date of Patent: December 16, 2008Assignee: University of Florida Research Foundation, Inc.Inventor: Yunwei Charles Cao
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Publication number: 20080175779Abstract: A method of forming rare earth oxide nanocrystals include the steps of dissolving a rare earth including compound in a solution containing at least one organic solvent, heating the solution to a temperature of at least 160° C., wherein a concentration of the rare earth including compound provided upon decomposition is sufficient to provide critical supersaturation of at least one active intermediate in the solution to nucleate a plurality of rare earth oxide nanocrystals. The plurality of rare earth nanocrystals are then grown, wherein the growing step proceeds at least in part in the absence of critical supersaturation of the active intermediate. The rare earth nanocrystals can assemble into at least one close-packed, ordered nanocrystal superlattice.Type: ApplicationFiled: March 30, 2005Publication date: July 24, 2008Inventor: Yunwei Charles Cao