Abstract: An automated apparatus for pre-processing of nucleic acid detection includes a reagent plate to place sample and pre-processing reagent; a base for placing the reagent plate provided with a temperature control module and a blending module; a magnetic separation module for magnetic particles separation, cleaning and elution; a pipetting device for transferring the reagent in the reagent plate comprising a pipetting module, a rotation module and a pipetting tip; a mobile rail platform for supporting the pipetting device; and a host computer for controlling the temperature control module, rotation module, blending module, magnetic separation module and pipetting module. The automated apparatus for pre-processing of nucleic acid detection of the present invention can execute full automation of pre-processing of nucleic acid detection including extraction, library construction and product purification. The final product can be directly used in the unattended operation of high-throughput sequencing.