Abstract: The present invention provides a composition, use of the composition in preparing drugs for treating and/or relieving depigmentation disorders, and a preparation comprising the composition. The composition comprises fluoxetine and vitamin D3 or derivatives of vitamin D3. In the composition, fluoxetine in combination with VD3 derivatives can improve melanogenesis. Compared with VD3 derivatives or fluoxetine when used alone, fluoxetine in combination with VD3 derivatives can not only improve the curative effect, but also reduce the dosage of VD3 derivatives or fluoxetine and the occurrence of toxic and side effects.