Abstract: Provided is an anti-Henipavirus monoclonal antibody having broad spectrum neutralization activity, wherein the antibody comprises a macaque variable region and a human constant region. The antibody of the present invention has good binding activity to both Nipah virus glycoprotein G and Hendra virus glycoprotein G, can effectively neutralize Nipahpseudovirus and Hendra pseudovirus, and can be used for preparing drugs for treating Henipavirus diseases.

Abstract: Disclosed in the present invention is an anti-Nipah virus monoclonal antibody having neutralization activity. The antibody consists of a monkey-derived variable region and a human constant region, and both light and heavy chains of the monkey-derived variable region have unique CDR regions. The antibody provided by the present invention has an excellent antigen binding capability, and has good binding activity with Bangladesh Nipah virus and Malaysia Nipah virus glycoprotein G. The antibody can effectively neutralize the Nipahpseudovirus. Moreover, the neutralization activity of the antibody is enhanced as the concentration of the antibody increases, and nearly 100% neutralization of the Nipahpseudovirus can be achieved at a concentration of 1 ?g/mL. Also disclosed in the present invention is an application of the monoclonal antibody against the Nipah virus glycoprotein G in preparation of a Nipah virus treatment drug.