Abstract: The present application discloses a method for improving density-tolerant yield of maize and use thereof, belongs to the field of plant biotechnology breeding. Compared with the Ig1 mutant strain, the LG1/Ig1 heterozygous genotype strain has excellent agronomic characters such as a widened leaf, a larger spike, a reduced empty stalk ratio and/or an increased yield by close planting; compared with the LG1 wild-type strain, the LG1/Ig1 heterozygous genotype material reduces a leaf angle and tassel branch number of maize, and has the effect of increasing the yield by close planting. The method has a wide application prospect in the field of maize high-yield breeding.

Abstract: Provided are ZmYUC2 and ZmYUC4 genes for regulating and controlling an included angle and the lodging resistance of a maize root system, and use thereof. The ZmYUC2 and ZmYUC4 genes can specifically regulate and control local auxin synthesis of a root tip, and can regulate and control the included angle of the maize root system without an adverse effect on the remaining agronomic traits, so that the ZmYUC2 and ZmYUC4 genes can be applied to a lodging-resistant breeding variety.

Abstract: A use of a ZmSBP12 gene in the regulation of drought resistance, plant height, and ear height of Zea mays L. is provided. After the ZmSBP12 gene is over-expressed in Zea mays L., the resulting Zea mays L. mutant plant exhibits increased drought resistance and decreased plant and ear heights. The overexpression of the ZmSBP12 gene leads to increased drought resistance and decreased plant and ear heights, indicating that the ZmSBP12 gene plays a crucial role in the drought resistance and plant type (plant height) of Zea mays L. The expression abundance of the ZmSBP12 gene is increased to improve the drought resistance of Zea mays L. and reduce the plant and ear heights of Zea mays L., which can be used for the assisted breeding of novel drought-resistant and lodging-resistant Zea mays L. varieties and the breeding of excellent inbred lines and hybrids of Zea mays L.

Abstract: A use of a ZmSBP12 gene in the regulation of drought resistance, plant height, and ear height of Zea mays L. is provided. After the ZmSBP12 gene is over-expressed in Zea mays L., the resulting Zea mays L. mutant plant exhibits increased drought resistance and decreased plant and ear heights. The overexpression of the ZmSBP12 gene leads to increased drought resistance and decreased plant and ear heights, indicating that the ZmSBP12 gene plays a crucial role in the drought resistance and plant type (plant height) of Zea mays L. The expression abundance of the ZmSBP12 gene is increased to improve the drought resistance of Zea mays L. and reduce the plant and ear heights of Zea mays L., which can be used for the assisted breeding of novel drought-resistant and lodging-resistant Zea mays L. varieties and the breeding of excellent inbred lines and hybrids of Zea mays L.

Abstract: A key DNA sequence for regulating a maize leaf angle and a mutant thereof are provided, which have polynucleotide sequences shown in SEQ ID No. 1 and SEQ ID No. 2, respectively. The DNA sequence for regulating a maize leaf angle and the mutant thereof provided by present disclosure can regulate the expression of ZmNAC16 gene in a maize pulvinus, and thus can be used for the improvement of maize leaf angle and plant type and further for the cultivation of new maize varieties. The present disclosure further provides specific detection primers for detecting mutations of the DNA key sequence and the mutant, and detection primers for detecting an expression level of ZmNAC16 gene in maize. These detection primers can be used to directionally improve a maize leaf angle and also shows application potential for breeding of dense-planting-tolerant and high-yield maize.