Abstract: A medical device according to the present invention is to be arranged in a lumen of a gastrointestinal tract. The medical device includes: at least one cylindrical part which is opened at least one end thereof; and at least one attachment part which is arranged on at least one portion of the cylindrical part and is configured such that the medical device is attachable to the lumen of the gastrointestinal tract. The cylindrical part is configured to be arrangeable along at least one portion of the gastrointestinal tract, and at least one portion of a side surface of the cylindrical part is configured to be followable to a shape of an inner wall of the gastrointestinal tract.

Abstract: A testing device according to the present invention includes a housing including a skin surface sheet and an upper surface cover. A hollow needle configured to interlock with the upper surface cover and a testing reagent or equipment are accommodated in the housing. Through deformation of the upper surface cover by application of an external force, the hollow needle perforates the skin. A body fluid flows in the space inside of the housing and is brought into contact with the testing reagent or equipment to acquire information on the body fluid. Through recovery of the upper surface cover to an original shape by elimination of the external force, the hollow needle again becomes accommodated in the housing.

Abstract: A medical device according to the present invention is to be arranged in a lumen of a gastrointestinal tract. The medical device includes: at least one cylindrical part which is opened at least one end thereof; and at least one attachment part which is arranged on at least one portion of the cylindrical part and is configured such that the medical device is attachable to the lumen of the gastrointestinal tract. The cylindrical part is configured to be arrangeable along at least one portion of the gastrointestinal tract, and at least one portion of a side surface of the cylindrical part is configured to be followable to a shape of an inner wall of the gastrointestinal tract.

Abstract: A testing device according to the present invention includes a housing including a skin surface sheet and an upper surface cover. A hollow needle configured to interlock with the upper surface cover and testing means are accommodated in the housing. Through deformation of the upper surface cover by application of an external force, the hollow needle perforates the skin. A body fluid flows in the space inside of the housing and is brought into contact with the testing means to acquire information on the body fluid. Through recovery of the upper surface cover to an original shape by elimination of the external force, the hollow needle again becomes accommodated in the housing.

Abstract: The present invention provides a process for producing biologically active recombinant yeast protein disulfide isomerase, which includes the steps of: a) deleting, substituting, or adding one or more bases in a region encoding an endoplasmic reticulum retention signal in a gene encoding protein disulfide isomerase of yeast to modify the gene so as not to encode part or all of the endoplasmic reticulum retention signal; b) incorporating the modified gene into an expression vector; c) transforming host cells with the expression vector; and d) culturing the host cells transformed with the expression vector in a culture medium kept at a nearly neutral pH, thereby causing protein disulfide isomerase to be secreted in an active state outside the host cells. According to the process of the invention, recombinant yeast protein disulfide isomerase can be secreted in a large amount in a biologically active state into a culture medium, and can be collected by a simple purification method.

Abstract: The present invention provides a method for producing nested deletions in vitro in desired DNA, comprising: 1) preparing a vector containing a DNA fragment in which nested deletions are to be generated and the terminal repeat of a transposon; 2) incubating the vector in vitro with transposase and a DNA replication system; 3) obtaining the vector incorporating the DNA fragment with nested deletions as a reaction product; and optionally, 4) transforming a host cell with the reaction product and growing it; and 5) recovering a vector incorporating said DNA fragment with nested deletions from the grown host cell.

Abstract: The present invention provides a gene encoding the type B subunit protein of avian lactate dehydrogenase. The gene encoding the type B subunit protein of avian lactate dehydrogenase of the present invention is obtained by plaque hybridization or the like from a cDNA library derived from avian heart muscle and encodes the type B subunit protein of avian lactate dehydrogenase having an amino acid sequence shown as SEQ ID NO. 2.

Abstract: The invention relates to a mutated human ALT (alanine aminotransferase) gene improving the DNA sequence encoding the N-terminal region of human ALT, without causing amino acid substitution, while, at the same time, adding restriction endonuclease sites to the upstream and downstream sites of the gene. By cultivation of E. coli transformant carrying a recombinant plasmid, which was constructed by ligation of the mutated human ALT gene into a vector, an active human ALT can be produced efficiently.

Abstract: The present invention relates to a method for producing human myoglobin by inserting a DNA sequence comprising a human myoglobin gene into a tryptophan promoter-containing vector, followed by making E. coli with the thus-obtained expression vector express a holo-type human myoglobin. Using this method, it is possible to directly and efficiently produce a heme-structured, holo-type human myoglobin by gene recombination technology.

Abstract: The present invention provides a gene encoding the type B subunit protein of avian lactate dehydrogenase. The gene encoding the type B subunit protein of avian lactate dehydrogenase of the present invention is obtained by plaque hybridization or the like from a cDNA library derived from avian heart muscle and encodes the type B subunit protein of avian lactate dehydrogenase having the sequences shown as SEQ ID NO. 1 or 2.