Abstract: [Problem to be solved] An object of the present invention is to provide a Raman spectroscopy analysis method that identifies the number of irradiation times of laser light at which a sample to be analyzed is damaged by the laser light during multiple Raman spectroscopy measurements and performs structural analysis of the sample to be analyzed using data on Raman scattering light obtained when the sample to be analyzed is in an undamaged state.

Abstract: An infrared Raman microscope capable of switching to and performing infrared spectroscopic analysis or Raman spectroscopic analysis for a sample on a stage includes an infrared light detection system, a Raman light detection system, a display unit, a display switching processing unit, and storage processing units. The infrared light detection system and the Raman light detection system photographs a visible image at different magnifications. The display unit displays the visible image of the sample in a display area in association with coordinates on the stage. The display switching processing unit switches and displays the visible image in the same image display area. In a case where a measurement position of the infrared spectroscopic analysis or the Raman spectroscopic analysis is designated on the display area, the storage processing units store a point of coordinates on the stage corresponding to the measurement position.

Abstract: An analysis result of an infrared spectrum at each measurement position is mapped and displayed in an infrared map display area as infrared data in association with a point of coordinates of each measurement position. An analysis result of a Raman spectrum at each measurement position is mapped and displayed in a Raman map display area as Raman data in association with a point of coordinates of each measurement position. In a case where an optional measurement position is designated in the infrared map display area and the Raman map display area, an infrared spectrum and a Raman spectrum associated with the designated measurement position are graphically displayed in the same graph display area.

Abstract: An infrared spectrum display processing unit performs processing of displaying an infrared spectrum obtained by infrared spectroscopic analysis in a graph representing a relationship between a wave number and intensity. A data conversion processing unit performs processing of converting a Raman spectrum obtained by Raman spectroscopic analysis into an equal interval Raman spectrum in which data points are plotted by wave numbers at equal intervals. A Raman spectrum display processing unit performs processing of displaying an equal interval Raman spectrum as a graph representing a relationship between a wave number and intensity.

Abstract: An image creation unit of a server creates and stores a plurality of phase images or the like having different resolutions on the basis of holographic data collected by a measuring terminal. In response to an image transmission request according to an operation, an image transmission processing unit of the server extracts data of an image corresponding to an observation range after the movement from an image of an appropriate resolution and transmits the data to the browsing terminal. In the browsing terminal, a display image is formed by overlaying a high-resolution phase image corresponding to only an observation range to be displayed on low-resolution phase image of an observation target area.

Abstract: The present invention is a cell observation device in which two-dimensional distributions of phase information and intensity information are computed based on hologram data obtained with a holographic microscope. An image display screen (100) displayed on a display unit includes an image display area (120) having two image display frames (121, 122). A phase image and intensity image corresponding to the same observation range on a cell culture plate (12), on which the cells to be observed are cultured, are displayed in the image display frames (121, 122), respectively. The wells on the plate, which are almost invisible on the phase image, are clearly visible on the intensity image. Conversely, the biological cells, which are almost invisible on the intensity image, are observable on the phase image. An observer specifies the range to be observed within a well on the intensity image, and subsequently enlarges the image corresponding to that range so as to observe the cells in detail on the phase image.

Abstract: A display image formation unit reads image data according to a designated condition from an image data storage unit to form a phase image, a drawing color setting unit colorizes a phase image according to a color scale in which a phase value in the vicinity of 0 is associated with black, a phase value in a positive direction is associated with a predetermined color other than black, and a phase value in a negative direction is associated with another color other than black. In an area where no cell exists on a medium, the phase value becomes almost 0, so that the background area of the cell on the colorized phase image becomes almost black and the cell is depicted in a color other than black.

Abstract: An image creation unit of a server creates and stores a plurality of phase images or the like having different resolutions on the basis of holographic data collected by a measuring terminal. In response to an image transmission request according to an operation, an image transmission processing unit of the server extracts data of an image corresponding to an observation range after the movement from an image of an appropriate resolution and transmits the data to the browsing terminal. In the browsing terminal, a display image is formed by overlaying a high-resolution phase image corresponding to only an observation range to be displayed on low-resolution phase image of an observation target area.