Abstract: A nano-fluidic device includes: a first substrate that has a nanoscale groove on one surface; and a second substrate that is integrally provided with the first substrate by bonding one surface of the second substrate to the one surface of the first substrate and forms a nanochannel with the groove of the first substrate, in which either the first substrate or the second substrate includes at least a thin portion in a part of a position overlapping the nanochannel in plan view, and the thin portion is deformed by pressing to open and close the nanochannel.

Abstract: A first proton-donating layer (20a) is a layer having a proton-donative functional group on the surface, for example, a silicon oxide layer. A second proton-donating layer (20b) is also a layer having a proton-donative functional group on the surface, for example, a silicon oxide layer. Negative surface charges are formed on the main surface section of a first base (10a) and the main surface section of a second base (10b), and these negative charges increased the proton conductivity in an aqueous solution fed to a nano channel. Although, in the aqueous solution, proton migration through hopping between water molecules contributes to its diffusion, the negative charges formed on the main surfaces of the bases (10a, 10b) attract protons in the aqueous solution, and the conduction of protons is efficiently achieved in “high-speed transfer regions” formed in the vicinity of the proton-donating layers (20a, 20b).

Abstract: The present disclosure provides an interface device that is capable of introducing a sample that has been ionized into a mass spectrometer with high efficiency. An ice droplet generating section forms ice droplets from a liquid sample that has been supplied from a sample supply section. Further, the ice droplet generating section successively introduces the formed ice droplets into an ionization section. The ionization section ionizes the sample that has been made into ice droplets, and conveys these ionized droplets into a mass spectrometer.

Abstract: A nano-fluidic device includes: a first substrate that has a nanoscale groove on one surface; and a second substrate that is integrally provided with the first substrate by bonding one surface of the second substrate to the one surface of the first substrate and forms a nanochannel with the groove of the first substrate, in which either the first substrate or the second substrate includes at least a thin portion in a part of a position overlapping the nanochannel in plan view, and the thin portion is deformed by pressing to open and close the nanochannel.

Abstract: A first proton-donating layer (20a) is a layer having a proton-donative functional group on the surface, for example, a silicon oxide layer. A second proton-donating layer (20b) is also a layer having a proton-donative functional group on the surface, for example, a silicon oxide layer. Negative surface charges are formed on the main surface section of a first base (10a) and the main surface section of a second base (10b), and these negative charges increased the proton conductivity in an aqueous solution fed to a nano channel. Although, in the aqueous solution, proton migration through hopping between water molecules contributes to its diffusion, the negative charges formed on the main surfaces of the bases (10a, 10b) attract protons in the aqueous solution, and the conduction of protons is efficiently achieved in “high-speed transfer regions” formed in the vicinity of the proton-donating layers (20a, 20b).

Abstract: A first and a second fluorescent dye are mixed into a solution, the first dye being positively ionized in the solution and the second dye being negatively ionized in the solution and having different fluorescence wavelengths from the first dye. The solution is flown onto a measured surface, and the surface is excited with an evanescent wave to produce a fluorescence intensity distribution of two colors. A fluorescence intensity of the surface is measured using a two-dimensional imaging element, the element providing a fluorescence intensity of each color separated from the other colors, thereby calculating a ratio of the fluorescence intensities of the colors. Using an equation expressing a relationship between the ratio of fluorescence intensities and a wall zeta potential, the ratio is converted to a two-dimensional distribution of wall zeta potentials. This achieves visualizing in real time and quantitatively evaluating the two-dimensional distribution of wall zeta potentials, and surface modifications.

Abstract: A first and a second fluorescent dye are mixed into a solution, the first dye being positively ionized in the solution and the second dye being negatively ionized in the solution and having different fluorescence wavelength from the first dye. The solution is flown onto a measured surface, and the surface is excited with an evanescent wave to produce a fluorescence intensity distribution of two colors. A fluorescence intensity of the surface is measured using a two-dimensional imaging element, the element providing a fluorescence intensity of each color separated from the other colors, thereby calculating a ratio of the fluorescence intensities of the colors. Using an equation expressing a relationship between the ratio of fluorescence intensities and wall zeta potential, the ratio is converted to a two-dimensional distribution of wall zeta potentials. This achieves visualizing in real time and quantitatively evaluating the two-dimensional distribution of wall zeta potentials, and surface modifications.

Abstract: The invention relates to a microscopic cell observation and inspection system that uses a total internal reflection cell illuminator that is capable of freely changing an observation position without recourse to any special slide glass, makes sure high SN-ratio observation and facilitates sample manipulation, thereby making high-sensitivity, fast detection of a lot of cell reactions on the same slide glass. While, in response to a command from personal computer (80), step motors (53, 54) are driven to sequentially scan observation positions of cell sample (S) on slide glass (21), one of shutter units (71) and (72) is closed and the other is opened at high speed, whereby either one of illumination optical paths for a TIRF microscope and a drop fluorescence microscope is selected to illuminate cell sample (S) on that observation position.

Abstract: The invention relates to a microscopic cell observation and inspection system that uses a total internal reflection cell illuminator that is capable of freely changing an observation position without recourse to any special slide glass, makes sure high SN-ratio observation and facilitates sample manipulation, thereby making high-sensitivity, fast detection of a lot of cell reactions on the same slide glass. While, in response to a command from personal computer (80), step motors (53, 54) are driven to sequentially scan observation positions of cell sample (S) on slide glass (21), one of shutter units (71) and (72) is closed and the other is opened at high speed, whereby either one of illumination optical paths for a TIRF microscope and a drop fluorescence microscope is selected to illuminate cell sample (S) on that observation position.