Patents by Inventor Yutaka Takarada
Yutaka Takarada has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7659060Abstract: A method for identifying a nucleotide polymorphism, the method comprising hybridizing a labeled oligonucleotide for a wild type or a mutant type to a nucleic acid containing a specific nucleotide polymorphic site in a sample; allowing a nucleic acid-specific label to act thereon; detecting an interaction between the label of the oligonucleotide and the nucleic acid-specific label; and identifying a nucleotide polymorphism, wherein the identification utilizes a difference due to the nucleotide polymorphism in conditions under which the hybridized oligonucleotide is separated into a single strand.Type: GrantFiled: September 2, 2003Date of Patent: February 9, 2010Assignee: Toyo Boseki Kabushiki KaishaInventors: Yutaka Takarada, Yoshihiro Soya, Yoshihisa Kawamura
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Patent number: 7601504Abstract: It is intended to provide a method of conveniently screening a novel protein or a novel partial sequence protein having a blocking ability based on amino acid sequence data; and a protein achieving an improved blocking efficiency that can be expressed on a large scale in Escherichia coli. A method of screening a novel protein or a novel partial sequence protein having a blocking ability based on amino acid sequence data; a protein characterized by achieving an improved blocking efficiency owing to an amino acid sequence modification; and a method of utilizing the protein.Type: GrantFiled: July 2, 2004Date of Patent: October 13, 2009Assignee: Toyo Boseki Kabushiki KaishaInventors: Toshihiro Kuroita, Atsushi Sogabe, Yutaka Takarada, Naoki Tanaka
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Publication number: 20070292853Abstract: A DNA array for detecting a single nucleotide polymorphism of a gene, which comprises, on a solid support, a first probe spots group consisting of one or more probe spots each containing one or more probes hybridizable with a polynucleotide of the gene, in which the probes are exactly complementary to the first polymorphism pattern of the gene, and a second probe spots group consisting of one or more probe spots each containing one or more probes hybridizable with the polynucleotide of the gene, in which the probes are exactly complementary to the second polymorphism pattern of the gene, wherein probe lengths in the probe spots is different from each other. This DNA array enables more exact SNP detection.Type: ApplicationFiled: March 18, 2005Publication date: December 20, 2007Applicants: TOYOBO CO., LTD., NGK INSULATORS, LTD.Inventors: Mitsuo Kawase, Yasuko Yoshida, Kazunari Yamada, Yutaka Takarada, Kouzo Hashimoto
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Publication number: 20060183173Abstract: It is intended to provide a method of conveniently screening a novel protein or a novel partial sequence protein having a blocking ability based on amino acid sequence data; and a protein achieving an improved blocking efficiency that can be expressed on a large scale in Escherichia coli. A method of screening a novel protein or a novel partial sequence protein having a blocking ability based on amino acid sequence data; a protein characterized by achieving an improved blocking efficiency owing to an amino acid sequence modification; and a method of utilizing the protein.Type: ApplicationFiled: July 2, 2004Publication date: August 17, 2006Applicant: Toyo Boseki Kabushiki KaishaInventors: Toshihiro Kuroita, Atsushi Sogabe, Yutaka Takarada, Naoki Tanaka
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Publication number: 20040191815Abstract: The array of the present invention has a plurality of double-stranded oligonucleotides immobilized on a metal substrate. Each of the double-stranded oligonucleotides includes a first single-stranded oligonucleotide and a second single-stranded oligonucleotide. The first and second single-stranded oligonucleotides are entirely or partially bonded together in a complementary manner to form said double-stranded oligonucleotide. Among the first and second single-stranded oligonucleotides, only the first single-stranded oligonucleotide is bonded on said substrate. The present invention also relates to a method for analyzing the interaction between the immobilized biomolecules and another biomolecule or an aggregate thereof by use of the array.Type: ApplicationFiled: January 14, 2004Publication date: September 30, 2004Inventors: Motoki Kyo, Masayuki Yamamoto, Hozumi Motohashi, Kinuko Ohneda, Yutaka Takarada, Bunsei Kawakami, Yoshihisa Kawamura
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Publication number: 20040121344Abstract: The present invention provides a method for detecting nucleotide polymorphism contained in a sample wherein nuclease activity and legase activity are employed at the same time.Type: ApplicationFiled: January 3, 2003Publication date: June 24, 2004Inventors: Yutaka Takarada, Toshiya Aono, Masaya Segawa, Satoko Yoshiga
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Publication number: 20040081991Abstract: A method for identifying a nucleotide polymorphism, the method comprising hybridizing a labeled oligonucleotide for a wild type or a mutant type to a nucleic acid containing a specific nucleotide polymorphic site in a sample; allowing a nucleic acid-specific label to act thereon; detecting an interaction between the label of the oligonucleotide and the nucleic acid-specific label; and identifying a nucleotide polymorphism, wherein the identification utilizes a difference due to the nucleotide polymorphism in conditions under which the hybridized oligonucleotide is separated into a single strand.Type: ApplicationFiled: September 2, 2003Publication date: April 29, 2004Applicant: Toyo Boseki Kabushiki KaishaInventors: Yutaka Takarada, Yoshihiro Soya, Yoshihisa Kawamura
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Publication number: 20030148301Abstract: A method of detecting variation or polymorphism in a nucleic acid sequence, which is useful particularly in diagnosing a hereditary disease, analyzing nucleotide polymorphism, etc., and primers to be used therein. More particularly speaking, this method comprises treating a primer for wild type and one or two primers for variant with DNA polymerase either simultaneously or separately, and detecting the nucleotide polymorphism contained in the nucleic acid sample by judging whether or not the primers are extended, or whether or not the primers are amplified with a reverse primer. In this method, the 3′-end second bases of the primer for wild type and one or two primers for variant correspond to respective nucleotides anticipated in the nucleotide polymorphism site. Further, at least one of the bases from the 3′-end third position to the 5′-end is substituted by a base not complementary to the base in the chain hybridized with the primer in the chromosome or fragment.Type: ApplicationFiled: October 17, 2002Publication date: August 7, 2003Inventors: Toshiya Aono, Yutaka Takarada, Masaya Segawa, Satoko Yoshiga
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Patent number: 6582922Abstract: The present invention provides a method of extracting and isolating nucleic acids from a material containing nucleic acids using a nucleic acid-binding particulate carrier. More specifically, the present invention provides a nucleic acid extraction method using a particulate carrier having a particle diameter of 0.5 to 15.0 &mgr;m, a pore diameter of 50 to 500 nm and a pore volume of 200 to 5000 mm3/g. According to the method of the invention, nucleic acids can be efficiently extracted from a biological material, in particular a material containing a large amount of contaminants, such as a clinical sample.Type: GrantFiled: February 7, 2002Date of Patent: June 24, 2003Assignee: Toyo Boseki Kabushiki KaishaInventors: Katsuya Daimon, Shigeru Komai, Yutaka Takarada
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Patent number: 6303306Abstract: In a method for amplifying the specific nucleic acid sequence, a highly specific amplification which has low possibility of non-specific hybridization can be carried out. Highly stable reagents, the activities of which do not decrease in case of supply and storage, are also provided. Thermostable enzymes are used as RNA dependent DNA polymerase, DNA dependent DNA polymerase, DNA dependent RNA polymerase and ribonuclease H, which are required for the amplification system based on replicated RNA. Especially, it is preferable that a thermostable enzyme derived from Thermus thermophilus which has RNA dependent DNA polymerase activity, DNA dependent DNA polymerase activity and ribonuclease H activity, and thermostable DNA dependent RNA polymerase are used together. By this method, inactivation of enzymes are prevented by using thermostable enzymes, and the amplification can be carried out without sequential addition of enzymes.Type: GrantFiled: April 15, 1999Date of Patent: October 16, 2001Assignee: Toyo Boseki Kabushiki KaishaInventors: Yutaka Takarada, Hiroaki Inoue, Shuji Shibata, Yoshihisa Kawamura
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Patent number: 6281008Abstract: This invention provides an apparatus for extracting nucleic acids from nucleic acid-containing samples. The nucleic acid extraction apparatus of the invention comprises (1) a group of extraction vessels each comprising a reactor tube in which a sample, a reagent solution, and a magnetic carrier are admixed and reacted, a drain cup for pooling an unwanted component solution, and a nucleic acid recovery tube all as secured to a support, (2) a distribution means for introducing a solution into each of the extraction vessels, (3) a stirring means for mixing the solution and magnetic carrier in the reactor tube, (4) a holding means for holding the magnetic carrier stationary within the vessel, (5) a discharging means for discharging the solution from the reactor tube while the magnetic carrier is held stationary, (6) a heating means for heating the solution and magnetic carrier in the reactor tube, and (7) a transfer means for serially transferring the vessels to the given positions.Type: GrantFiled: February 1, 1999Date of Patent: August 28, 2001Assignee: Toyo Boseki Kabushiki KaishaInventors: Shigeru Komai, Katsuya Daimon, Yutaka Takarada
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Patent number: 6261773Abstract: The present invention provide a process for sequence-specific nucleic acid amplification capable of improving the detection sensitivity and increasing the signal. In particular, the present invention provides a reagent for nucleic acid amplification containing at least one member selected from the group consisting of EDTA, NTA, UDA, CyDTA, DTPA, GEDTA, TTHA and their salts, specifically a reagent for nucleic acid amplification comprising, in addition to the at least one compound, a forward primer having a DNA sequence homologous to a sequence of a target RNA; a reverse primer having a DNA sequence complementary to a sequence of the target RNA and having a promoter for RNA polymerase attached to its 5′ end; ribonucleotides; deoxyribonucleotides; a reverse transcriptase or RNA-directed DNA polymerase; a RNase H; a DNA polymerase or a reverse transcriptase having DNA-directed DNA polymerase activity; and a RNA polymerase.Type: GrantFiled: March 16, 1999Date of Patent: July 17, 2001Assignee: Toyo Boseki Kabushiki KaishaInventors: Masaya Segawa, Motohiro Kondo, Yutaka Takarada
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Patent number: 6255478Abstract: This invention provides an apparatus for extracting nucleic acids from nucleic acid-containing samples, particularly biological samples, and more particularly to a nucleic acid extraction apparatus well suited for the nucleic acid extraction method utilizing a nucleic acid-binding magnetic carrier.Type: GrantFiled: January 3, 2000Date of Patent: July 3, 2001Assignee: Toyo Boseki Kabushiki KaishaInventors: Shigeru Komai, Katsuya Daimon, Yutaka Takarada
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Patent number: 5981183Abstract: In a method for amplifying the specific nucleic acid sequence, a highly specific amplification which has low possibility of non-specific hybridization can be carried out. Highly stable reagents, the activities of which do not decrease in case of supply and storage, are also provided. Thermostable enzymes are used as RNA dependent DNA polymerase, DNA dependent DNA polymerase, DNA dependent RNA polymerase and ribonuclease H, which are required for the amplification system based on replicated RNA. Especially, it is preferable that a thermostable enzyme derived from Thermus thermophilus which has RNA dependent DNA polymerase activity, DNA dependent DNA polymerase activity and ribonuclease H activity, and thermostable DNA dependent RNA polymerase are used together. By this method, inactivation of enzymes are prevented by using thermostable enzymes, and the amplification can be carried out without sequential addition of enzymes.Type: GrantFiled: March 20, 1997Date of Patent: November 9, 1999Assignee: Toyo Boseki Kabushiki KaishaInventors: Yutaka Takarada, Hiroaki Inoue, Shuji Shibata, Yoshihisa Kawamura
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Patent number: 5525462Abstract: An oligonucleotide is provided, which has at least a base sequence A, which is 10-30 nucleotides in length and homologous to a part of a specific nucleic acid sequence to be amplified, and a base sequence B, which is 10-30 nucleotides in length and complementary to a sequence 3' to the part of the specific nucleic acid sequence to be amplified. Sequences A and B are separated by a spacer region of 0-20 nucleotides. The oligonucleotide is used in an amplification method, wherein the oligonucleotide is mixed with a sample containing a specific nucleic acid sequence to be amplified and allowed to anneal to the specific nucleic acid sequence in the sample. The annealed oligonucleotide acts as a primer in an elongation reaction, whereas any nonannealed oligonucleotide is decomposed, except for at least part of the base sequence A. The elongation product is then denatured to a single strand for use as a template to which the remaining oligonucleotide anneals to form a primer for subsequent elongation.Type: GrantFiled: April 28, 1992Date of Patent: June 11, 1996Assignee: Toyo Boseki Kabushiki KaishaInventors: Yutaka Takarada, Toshiya Aono, Shuji Shibata