Abstract: Provided is a gasket for an automobile fuel tank that is attached to a connection part in which a fuel supply pipe is connected to an automobile fuel tank and can suppress permeation of gasoline more effectively. Along an inner periphery or an outer periphery of an O-ring 2 made of nitrile-butadiene rubber, a cross-sectionally rectangular annular member 3 made of polytetrafluoroethylene is arranged in close contact with the O-ring 2, and the O-ring 2 is protruded in thickness direction relative to the upper and lower surfaces in the thickness direction of the annual member 3 by allowing a thickness of the O-ring 2 to be larger than the thickness of the annular member 3.

Abstract: An object of the present invention is not to delete certain nerve cell by irreversible cell death, but to provide a very effective tool for analyzing the network of the cerebral nervous system by controlling reversibly the expression of a protein having a synaptic transmission-controlling activity in certain nerve cell. The present invention provides a DNA which comprises a DNA encoding a protein having a synaptic transmission-controlling activity and has a construct capable of controlling reversibly the expression of the protein having a synaptic transmission-controlling activity in certain nerve cell.

Abstract: An object of the present invention is to provide a transgenic animal which is useful in a test for forecasting fetal toxicity and teratogenicity of drugs. The present invention provides a transgenic non-human animal or a part thereof wherein several foreign (human-type) proteins which are involved in the drug metabolism or mutant proteins thereof are expressed in the body; a recombinant gene which is useful for the production of said non-human animal; and a method for screening a substance having fetal toxicity and teratogenicity using said non-human animal.

Abstract: The lubricant layer according to the present invention is used in a thin film magnetic recording medium in which a solid surface sliding at a high speed is required to retain its lubricating performance and abrasion resistance for a long period of time, comprising a host-guest complex composed of a lubricant molecule as a guest molecule and a multidentate ligand as a host compound to form the complex with the guest molecule.

Abstract: The lubricant layer according to the present invention is used in a thin film magnetic recording medium in which a solid surface sliding at a high speed is required to retain its lubricating performance and abrasion resistance for a long period of time, comprising a host-guest complex composed of a lubricant molecule as a guest molecule and a multidentate ligand as a host compound to form the complex with the guest molecule.

Abstract: A novel chondromodulin-I protein having a molecular weight of about 26,000 dalton on SDS-PAGE and capable of stimulating the growth of chondrocytes with or without FGF and promoting the differential potency of said cells, a DNA encoding said protein, expression vector containing said DNA, a transformant capable of producing recombinant chondromodulin-I protein, a process for producing chondromodulin-I protein by culturing said transformant and a pharmaceutical composition containing chondromodulin-I protein as an active ingredient.

Abstract: The present invention provides a GTP binding protein containing the following amino acid sequence, with a molecular weight of about 22K dalton and having GTP binding activity which is inhibited by N-ethyl-maleimide and GTP hydrolyzing activity:Thr-Ile-Glu-Asp-Ser-Tyr, and a method for the production of a GTP binding protein, which comprises introducing a DNA fragment containing DNA that encodes the GTP binding protein into a cloning site present at the downstream to a promoter of an expression vector, then introducing the expression vector thus constructed into a host, culturing said host, thereby expressing and accumulating the GTP binding protein and then collecting thereof.

Abstract: A novel water-soluble enzyme protein being composed of three heterogeneous subunits and having nitrile hydratase activity was separated and purified from the bacterium of genus Rhizobium. The nucleotide sequence of the gene encoding the protein was determined; using the transformant transformed with the expression vector having the nucleotide sequence, the protein was expressed; by reacting the transformant with a nitrile in a culture broth, the corresponding amide was produced.Via the use of a culture broth containing the transformant described above or the protein itself, a nitrile can be efficiently converted into the corresponding amide.

Abstract: A novel chondromodulin-II protein having a molecular weight of about 16,000 dalton on SDS-PAGE, capable of stimulating the growth of chondrocytes with or without fibroblast growth factor and promoting the differential potency of said cells, and a pharmaceutical composition containing said protein as an active ingredient.

Abstract: The invention provides DNA fragments coding for human apolipoprotein E or human apolipoprotein E-like substances having physiological activities equivalent to those of said human apolipoprotein E, expression vectors suitable for production of such proteins, hosts for use in the production, and process for the production, as well as such proteins thereby produced.

Abstract: Provided herein are a DNA fragment which contains a base sequence coding for a non-A non-B hepatitis-specific antigenic protein occurring in cells of the liver affected with non-A non-B hepatitis, an expression vector in which said DNA fragment is inserted into a cloning site present downstream from a promoter thereof, a transformant obtained by introducing said expression vector into a host, and a process for producing said antigenic protein which comprises providing said expression vector, transforming a host with said expression vector, culturing the transforming host and collecting the protein produced therein.

Abstract: A recombinant DNA vector containing a DNA sequence which encodes a peptide having the physiological properties of cardionatrin ad cardiodilatin.