Abstract: It has been found that wild-type ferulic acid decarboxylase derived from Saccharomyces has high catalytic activity for the production of unsaturated hydrocarbon compounds. Further, it has been found that in the ferulic acid decarboxylase, substitute of the amino acid at position 398 with glutamine, methionine, asparagine, phenylalanine, histidine, or threonine more improves the catalytic activity, making it possible to provide a method capable of producing an unsaturated hydrocarbon compound such as butadiene with high productivity, and an enzyme used in the method.

Abstract: With the aim of providing a method allowing production of an unsaturated hydrocarbon compound such as butadiene with high productivity and an enzyme used in the method, the present inventors introduced mutations involving amino acid replacement into various positions of a ferulic acid decarboxylase, and prepared multiple modified forms of the enzyme. Then, the present inventors evaluated those modified forms in terms of the catalytic activity for the production of butadiene, and found as a result that the catalytic activity was improved in the case where, for example, the amino acid at position 395 was glutamine, histidine, asparagine, leucine, isoleucine, methionine, lysine, serine, arginine, tyrosine, or phenylalanine.

Abstract: With the aim of providing a method allowing production of an unsaturated hydrocarbon compound such as butadiene with high productivity and an enzyme used in the method, the present inventors introduced mutations involving amino acid replacement into various positions of a ferulic acid decarboxylase, and prepared multiple modified forms of the enzyme. Then, the present inventors evaluated those modified forms in terms of the catalytic activity for the production of butadiene, and found as a result that the catalytic activity was improved in the case where, for example, the amino acid at position 395 was glutamine, histidine, asparagine, leucine, isoleucine, methionine, lysine, serine, arginine, tyrosine, or phenylalanine.

Abstract: Disclosed is a connector mateable with a mating connector along a first direction. The connector comprises a housing and a plurality of terminals, wherein the housing includes a plurality of held portions, and the terminals correspond to the held portions, respectively. Each of the terminals includes a base portion, a first portion, a second portion and a third portion. The first portion extends directly from the base portion and is provided with a first contact portion which projects towards the second portion in a second direction perpendicular to the first direction. The second portion extends directly from the base portion and is provided with a second contact portion and a press-fitting projection. The second contact portion projects towards the first portion in the second direction and faces the first contact portion. The press-fitting projection projects towards the third portion in the second direction. The third portion extends directly from the base portion.

Abstract: Disclosed is a connector mateable with a mating connector along a first direction. The connector comprises a housing and a plurality of terminals, wherein the housing includes a plurality of held portions, and the terminals correspond to the held portions, respectively. Each of the terminals includes a base portion, a first portion, a second portion and a third portion. The first portion extends directly from the base portion and is provided with a first contact portion which projects towards the second portion in a second direction perpendicular to the first direction. The second portion extends directly from the base portion and is provided with a second contact portion and a press-fitting projection. The second contact portion projects towards the first portion in the second direction and faces the first contact portion. The press-fitting projection projects towards the third portion in the second direction. The third portion extends directly from the base portion.