Patents by Inventor Yuu UTASHIMA
Yuu UTASHIMA has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 11072809Abstract: An object of the present invention is to construct a more excellent glucose sensor, and to provide GDH more suitable for the glucose sensor. Provided is FAD-dependent glucose dehydrogenase in which the range of molecular weight distribution observed by SDS-PAGE is within 50 kDa when viewed in a molecular weight distribution in which the relative value of band intensity exceeds 60% of the maximum value.Type: GrantFiled: July 14, 2017Date of Patent: July 27, 2021Assignee: TOYOBO CO., LTD.Inventor: Yuu Utashima
-
Patent number: 10913971Abstract: Provided is GDH with increased applicability to glucose sensors. A composition contains FADGDH, wherein when 0.1 mL of the composition is added to 2.9 mL of a solution containing 10 mM of trehalose and 1 mmol/L of potassium ferricyanide to give a glucose dehydrogenase activity of 500 U/mL and incubated at 37° C., the decrease in absorbance at 405 nm resulting from reduction of the potassium ferricyanide is less than 20 mAbs per minute.Type: GrantFiled: April 7, 2016Date of Patent: February 9, 2021Assignee: TOYOBO CO., LTD.Inventors: Yuu Utashima, Takahide Kishimoto
-
Publication number: 20180066302Abstract: Provided is GDH with increased applicability to glucose sensors. A composition contains FADGDH, wherein when 0.1 mL of the composition is added to 2.9 mL of a solution containing 10 mM of trehalose and 1 mmol/L of potassium ferricyanide to give a glucose dehydrogenase activity of 500 U/mL and incubated at 37° C., the decrease in absorbance at 405 nm resulting from reduction of the potassium ferricyanide is less than 20 mAbs per minute.Type: ApplicationFiled: April 7, 2016Publication date: March 8, 2018Applicant: TOYOBO CO., LTD.Inventors: Yuu UTASHIMA, Takahide KISHIMOTO
-
Publication number: 20180030498Abstract: An object of the present invention is to construct a more excellent glucose sensor, and to provide GDH more suitable for the glucose sensor. Provided is FAD-dependent glucose dehydrogenase in which the range of molecular weight distribution observed by SDS-PAGE is within 50 kDa when viewed in a molecular weight distribution in which the relative value of band intensity exceeds 60% of the maximum value.Type: ApplicationFiled: July 14, 2017Publication date: February 1, 2018Applicant: TOYOBO CO., LTD.Inventor: Yuu UTASHIMA
-
Patent number: 9796963Abstract: The present invention provides a novel glucose dehydrogenase that has excellent substrate specificity and that is suitable for use in SMBG. The present invention provides a flavin-bound glucose dehydrogenase having the following characteristics (1) to (5): (1) Temperature stability: stable at a temperature of 45° C. or lower; (2) stable at a pH range of 4.5 to 7.5; (3) substrate specificity: the reactivity to D-xylose, maltose, or D-galactose is 2% or less, based on the reactivity to D-glucose taken as 100%; (4) optimal activity temperature: 34 to 47° C.; and (5) optimal activity pH: 6.3 to 6.7.Type: GrantFiled: September 6, 2013Date of Patent: October 24, 2017Assignee: Toyobo Co., Ltd.Inventors: Yasuhiro Yamazaki, Yosuke Sumida, Rie Hirao, Yuu Utashima, Hiroshi Aiba, Takahide Kishimoto, Shusaku Yanagidani
-
Patent number: 9657325Abstract: Provided is a flavin adenine dinucleotide-dependent glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 78% or more identity to the amino acid sequence of SEQ ID NO: 3, and having glucose dehydrogenase activity.Type: GrantFiled: April 21, 2016Date of Patent: May 23, 2017Assignee: Toyobo Co., Ltd.Inventors: Hiroshi Aiba, Yosuke Sumida, Yasuhiro Yamazaki, Yuu Utashima, Takahide Kishimoto
-
Patent number: 9506042Abstract: The present invention provides a novel glucose dehydrogenase that has excellent substrate specificity, specific activity, thermal stability, and the like, and that is suitable for use in SMBG sensors. The present invention provides a purified polypeptide comprising an amino acid sequence having at least 80% identity to the sequence of SEQ ID NO: 1 and having glucose dehydrogenase activity.Type: GrantFiled: December 29, 2014Date of Patent: November 29, 2016Assignee: Toyobo Co., Ltd.Inventors: Yosuke Sumida, Rie Hirao, Yuu Utashima, Hiroaki Kitazawa, Hiroshi Aiba, Takahide Kishimoto, Shusaku Yanagidani
-
Patent number: 9487758Abstract: An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. An isolated flavin-binding glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having glucose dehydrogenase activity is provided.Type: GrantFiled: February 10, 2014Date of Patent: November 8, 2016Assignee: Toyobo Co., Ltd.Inventors: Yosuke Sumida, Hiroshi Aiba, Hiroshi Kawaminami, Rie Hirao, Yuu Utashima, Takahide Kishimoto, Shusaku Yanagidani
-
Publication number: 20160265021Abstract: Provided is a flavin adenine dinucleotide-dependent glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 78% or more identity to the amino acid sequence of SEQ ID NO: 3, and having glucose dehydrogenase activity.Type: ApplicationFiled: April 21, 2016Publication date: September 15, 2016Applicant: TOYOBO CO., LTD.Inventors: Hiroshi AIBA, Yosuke SUMIDA, Yasuhiro YAMAZAKI, Yuu UTASHIMA, Takahide KISHIMOTO
-
Patent number: 9404144Abstract: An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. The flavin-binding glucose dehydrogenase of the invention has the following characteristics (1) and (4): (1) Molecular weight: the molecular weight of a polypeptide moiety in the enzyme is about 68 kDa as measured by SDS-polyacrylamide electrophoresis; (2) Km value: the Km value for D-glucose is about 15 mM or less; (3) Temperature stability: stable at a temperature of 55° C. or less; and (4) pH stability: stable at a pH range of 3.0 to 8.5.Type: GrantFiled: February 7, 2013Date of Patent: August 2, 2016Assignee: TOYOBO CO., LTD.Inventors: Yosuke Sumida, Rie Hirao, Yuu Utashima, Hiroshi Kawaminami, Hiroshi Aiba, Takahide Kishimoto, Shusaku Yanagidani
-
Patent number: 9260699Abstract: An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. An isolated flavin-binding glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having glucose dehydrogenase activity is provided.Type: GrantFiled: April 30, 2014Date of Patent: February 16, 2016Assignee: Toyobo Co., Ltd.Inventors: Yosuke Sumida, Hiroshi Aiba, Hiroshi Kawaminami, Rie Hirao, Yuu Utashima, Takahide Kishimoto, Shusaku Yanagidani
-
Publication number: 20150240216Abstract: The present invention provides a novel glucose dehydrogenase that has excellent substrate specificity and that is suitable for use in SMBG. The present invention provides a flavin-bound glucose dehydrogenase having the following characteristics (1) to (5): (1) Temperature stability: stable at a temperature of 45° C. or lower; (2) stable at a pH range of 4.5 to 7.5; (3) substrate specificity: the reactivity to D-xylose, maltose, or D-galactose is 2% or less, based on the reactivity to D-glucose taken as 100%; (4) optimal activity temperature: 34 to 47° C.; and (5) optimal activity pH: 6.3 to 6.7.Type: ApplicationFiled: September 6, 2013Publication date: August 27, 2015Inventors: Yasuhiro Yamazaki, Yosuke Sumida, Rie Hirao, Yuu Utashima, Hiroshi Aiba, Takahide Kishimoto, Shusaku Yanagidani
-
Patent number: 9040257Abstract: The present invention provides a host for genetic recombination useful in the production of heterologous proteins in a large scale, by suppressing the production of extracellular polysaccharides in the basidiomycetous yeasts. Cryptococcus sp. S-2 D11 strain (FERM BP-11482) which is characterized in that the production of extracellular polysaccharides is suppressed as compared with the parent strain.Type: GrantFiled: April 23, 2013Date of Patent: May 26, 2015Assignees: TOYOBO CO., LTD., NATIONAL RESEARCH INSTITUTE OF BREWINGInventors: Yuu Utashima, Takahide Kishimoto, Shusaku Yanagidani, Kazuo Masaki
-
Publication number: 20150111280Abstract: The present invention provides a novel glucose dehydrogenase that has excellent substrate specificity, specific activity, thermal stability, and the like, and that is suitable for use in SMBG sensors. The present invention provides a purified polypeptide comprising an amino acid sequence having at least 80% identity to the sequence of SEQ ID NO: 1 and having glucose dehydrogenase activity.Type: ApplicationFiled: December 29, 2014Publication date: April 23, 2015Inventors: Yosuke SUMIDA, Rie HIRAO, Yuu UTASHIMA, Hiroaki KITAZAWA, Hiroshi AIBA, Takahide KISHIMOTO, Shusaku YANAGIDANI
-
Publication number: 20150072379Abstract: The present invention provides a host for genetic recombination useful in the production of heterologous proteins in a large scale, by suppressing the production of extracellular polysaccharides in the basidiomycetous yeasts. Cryptococcus sp. S-2 D11 strain (FERN BP-11482) which is characterized in that the production of extracellular polysaccharides is suppressed as compared with the parent strain.Type: ApplicationFiled: April 23, 2013Publication date: March 12, 2015Applicants: TOYOBO CO., LTD., NATIONAL RESEARCH INSTITUTE OF BREWINGInventors: Yuu Utashima, Takahide Kishimoto, Shusaku Yanagidani, Kazuo Masaki
-
Publication number: 20150031059Abstract: An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. The flavin-binding glucose dehydrogenase of the invention has the following characteristics (1) and (4): (1) Molecular weight: the molecular weight of a polypeptide moiety in the enzyme is about 68 kDa as measured by SDS-polyacrylamide electrophoresis; (2) Km value: the Km value for D-glucose is about 15 mM or less; (3) Temperature stability: stable at a temperature of 55° C. or less; and (4) pH stability: stable at a pH range of 3.0 to 8.5.Type: ApplicationFiled: February 7, 2013Publication date: January 29, 2015Applicant: TOYOBO CO., LTD.Inventors: Yosuke Sumida, Rie Hirao, Yuu Utashima, Hiroshi Kawaminami, Hiroshi Aiba, Takahide Kishimoto, Shusaku Yanagidani
-
Publication number: 20140287478Abstract: An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. An isolated flavin-binding glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having glucose dehydrogenase activity is provided.Type: ApplicationFiled: April 30, 2014Publication date: September 25, 2014Inventors: Yosuke SUMIDA, Hiroshi AIBA, Hiroshi KAWAMINAMI, Rie HIRAO, Yuu UTASHIMA, Takahide KISHIMOTO, Shusaku YANAGIDANI
-
Publication number: 20140154777Abstract: An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. An isolated flavin-binding glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having glucose dehydrogenase activity is provided.Type: ApplicationFiled: February 10, 2014Publication date: June 5, 2014Applicant: TOYOBO CO., LTD.Inventors: Yosuke SUMIDA, Hiroshi AIBA, Hiroshi KAWAMINAMI, Rie HIRAO, Yuu UTASHIMA, Takahide KISHIMOTO, Shusaku YANAGIDANI