Abstract: There are provided systems and methods comprising obtaining an image of at least a part of an item, obtaining data informative of a pixel intensity of the part of the item in the image, obtaining first data informative of a pixel intensity in an image of a part of a first item associated with a prohibited material, wherein the part of the first item meets a similarity criterion with the part of the item, obtaining second data informative of a pixel intensity in an image of a part of a second item which is not associated with a prohibited material, wherein the part of the second item meets the similarity criterion with the part of the item, and using the first data, the second data and the data to determine whether the part of the item is associated with a concealed prohibited material in the image.