Abstract: A biological polypeptide applied to inhibition of intracellular lipid accumulation and a synthesis method thereof are provided. An amino acid sequence of the polypeptide is shown in SEQ ID NO: 1 in sequence listing. The polypeptide has relatively high target affinity and high activity, and can exhibit remarkably high activity at extremely low dosages and concentrations. The polypeptide inhibits binding of clusterin and sterol O-acyltransferase by exerting competition on the clusterin to reduce the activity of the sterol O-acyltransferase, so as to achieve functions of controlling physiological metabolism in organisms, such as relieving lipid accumulation in hepatocytes, resisting tumor occurrence, assisting anti-cancer drugs in inhibiting tumor growth, and the like.

Abstract: The present invention relates to the detection of promoter methylation status using a combination of either modification of methylated DNA or restriction endonuclease digestion, multiplex polymerase chain reaction, ligase detection reaction, and a universal array or capillary electrophoresis detection.

Abstract: The present invention relates to the detection of promoter methylation status using a combination of either modification of methylated DNA or restriction endonuclease digestion, multiplex polymerase chain reaction, ligase detection reaction, and a universal array or capillary electrophoresis detection.