Abstract: Disclosed are a two-layer microfluidic chip with magnetic bead luminescence and a detection system. The chip comprises a top plate and a bottom plate. The top plate comprises a sample addition section, a conjugated ligand storage section, and a sample mixing area, wherein the sample mixing area is in communication respectively with the sample addition section and the conjugated ligand storage section. The bottom plate comprises a flow guiding area, a magnetic bead coating section, a washing area, a detection area, and a washing fluid storage section, wherein the flow guiding area is arranged with a recess that is lower than the bottom wall of the magnetic bead coating section in the height direction, and a flow guiding portion fitted to the recess and connecting the magnetic bead coating section.

Abstract: Disclosed are a magnetic particle luminescent micro-fluidic chip for multi-marker detection and a detection apparatus. The chip includes a top plate, provided with a sample loading part and a mixing area with a plurality of labeling ligands; a bottom plate, including a flow guide area, a plurality of reaction areas communicated with a plurality of detection areas, a cleaning liquid storage part and a luminescent liquid storage part which are communicated with the detection areas, where each reaction area is provided with a different magnetic particle ligand; a cleaning liquid is stored in the cleaning liquid storage part, and a luminescent liquid is stored in the luminescent liquid storage part; and an air pump, disposed on the top plate and configured to drive a sample in the sample loading part to flow through the mixing area.