Abstract: The present disclosure provides plasmid DNA or a DNA/cationic substance complex containing a Rep and an ITR and exhibiting high AAV vector productivity. More specifically, in one exemplary aspect, the present disclosure provides a nucleic acid containing a Rep gene derived from an adeno-associated virus (AAV) of a first serotype, and an inverted terminal repeat (ITR) sequence derived from an AAV of a second serotype different from the first serotype and having a ?G value in a range of ?65 to ?95 kcal/mol.

Abstract: The present disclosure provides control of expression of a Rep gene by control of expression of a helper gene. In one aspect, the present disclosure provides a method for controlling the expression of a Rep gene, including a step of controlling the expression of a helper gene. In one embodiment, the helper gene includes a gene selected from adenovirus E1B, E2, E4 and VA. In one embodiment, the helper gene includes an adenovirus E1B gene. In one embodiment, the helper gene includes two or more helper genes related to the production of an adeno-associated virus (AAV)-derived construct, and optionally, the helper genes can be selected from E1A, E1B, E2, E4, and VA. In one embodiment, the helper gene includes a helper gene selected from E1A and E1B and a helper gene selected from E2, E4 and VA.

Abstract: The present disclosure provides production of a virus-derived construct plasmid. In one aspect, the present disclosure provides a plasmid encoding at least some elements of a virus-derived construct. In one embodiment, the present disclosure provides a plasmid containing at least some of nucleic acid sequences required to construct a virus-derived construct in an operably linked form. In one embodiment, the nucleic acid sequence required to construct a virus-derived construct includes (A) a nucleic acid sequence including a terminal repeat, (B) a nucleic acid sequence encoding a packaging factor, (C) a nucleic acid sequence encoding a structural protein, and (D) a nucleic acid sequence encoding a functional cofactor.

Abstract: In one aspect, the present disclosure provides a method for producing various virus-derived construct plasmids from starting plasmids (for example, virus-derived construct plasmids of the present disclosure).

Abstract: A method for manufacturing a stator for a rotary electric machine includes an installation step of installing coil pieces for a stator coil in a stator core, and a joining step of joining ends of the coil pieces or the end of the coil piece and an end of a busbar by laser welding after the installation step. The joining step includes a setting step of bringing two ends to be joined into contact with each other, an image recognition step of detecting, after the setting step, image features related to non-contact surfaces continuous with contact surfaces of the two ends in an image obtained by imaging the two ends, and a determination step of determining, after the image recognition step, a laser radiation position based on the image features related to the non-contact surfaces subjected to image recognition.

Abstract: A crystal resonator includes a flat plate-shaped crystal element and excitation electrodes. The crystal element has principal surfaces parallel to an X?-axis and a Z?-axis. The X?-axis is an axis of rotating an X-axis in a range of 15 to 25 degrees around a Z-axis. The Z?-axis is an axis of rotating the Z-axis in a range of 33 to 35 degrees around the X?-axis. The excitation electrodes are formed on the respective principal. The excitation electrodes include a first region with a circular outer shape and a second region. The second region is formed at a peripheral area of the first region. The second region has a thickness thinner than the first region and has an elliptical outer shape. The elliptical shape has a long axis extending in a direction in a range of ?5 to +15 degrees with respect to a direction that the X?-axis extends.

Abstract: A crystal resonator includes a crystal element and excitation electrodes. The crystal element has a pair of principal surfaces parallel to an X?-axis and a Z?-axis. The X?-axis is an axis of rotating an X-axis as a crystallographic axis of a crystal in a range of 15 degrees to 25 degrees around a Z-axis as a crystallographic axis of the crystal. The Z?-axis is an axis of rotating the Z-axis in a range of 33 degrees to 35 degrees around the X?-axis. The excitation electrodes are formed on the respective principal surfaces of the crystal element. Elliptical mesa portions or elliptical inverted mesa portions are formed on the respective principal surfaces. The mesa portions project from outer peripheries of the principal surfaces. The inverted mesa portions are depressed from the outer peripheries of the principal surfaces.

Abstract: A crystal resonator includes a crystal element and excitation electrodes. The crystal element has a pair of principal surfaces parallel to an X?-axis and a Z?-axis. The X?-axis is an axis of rotating an X-axis as a crystallographic axis of a crystal in a range of 15 degrees to 25 degrees around a Z-axis as a crystallographic axis of the crystal. The Z?-axis is an axis of rotating the Z-axis in a range of 33 degrees to 35 degrees around the X?-axis. The excitation electrodes are formed on the respective principal surfaces of the crystal element. Elliptical mesa portions or elliptical inverted mesa portions are formed on the respective principal surfaces. The mesa portions project from outer peripheries of the principal surfaces. The inverted mesa portions are depressed from the outer peripheries of the principal surfaces.

Abstract: A crystal resonator includes a flat plate-shaped crystal element and excitation electrodes. The crystal element has principal surfaces parallel to an X?-axis and a Z?-axis. The X?-axis is an axis of rotating an X-axis in a range of 15 to 25 degrees around a Z-axis. The Z?-axis is an axis of rotating the Z-axis in a range of 33 to 35 degrees around the X?-axis. The excitation electrodes are formed on the respective principal. The excitation electrodes include a first region with a circular outer shape and a second region. The second region is formed at a peripheral area of the first region. The second region has a thickness thinner than the first region and has an elliptical outer shape. The elliptical shape has a long axis extending in a direction in a range of ?5 to +15 degrees with respect to a direction that the X?-axis extends.

Abstract: A crystal resonator includes a flat plate-shaped crystal element and excitation electrodes. The crystal element has principal surfaces parallel to an X?-axis and a Z?-axis. The X?-axis is an axis of rotating an X-axis as a crystallographic axis of a crystal in a range of 15 degrees to 25 degrees around a Z-axis as a crystallographic axis of the crystal. The Z?-axis is an axis of rotating the Z-axis in a range of 33 degrees to 35 degrees around the X?-axis. The excitation electrodes are formed on the respective principal surfaces of the crystal element. The excitation electrodes are each formed into an elliptical shape. The elliptical shape has a long axis extending in a direction in a range of ?5 degrees to +15 degrees with respect to a direction that the X?-axis extends.

Abstract: An exemplary piezoelectric device includes a base having a first connection electrode electrically connected to an external electrode. A second connection electrode is connected to the first connection electrode on a first main surface of a supporting member affixed to the base. A third connection electrode, formed on a second main surface of the supporting member, is electrically connected to the second connection electrode. A piezoelectric vibrating piece is affixed to the supporting member by application of an adhesive applied to the third connection electrode. The piezoelectric vibrating piece and includes excitation electrodes. A convexity is located and extends in a designated direction relative to the third connection electrode to prevent applied adhesive from spreading beyond a designated area of the piezoelectric vibrating piece.