Abstract: An object of the present invention is to provide a method for efficiently separating insoluble bodies of a recombinant protein from a recombinant cell expressing a target recombinant protein as insoluble bodies in the cell. The present invention provides a method for producing a recombinant protein aggregate by separating insoluble bodies of a recombinant protein from a recombinant cell expressing the recombinant protein as insoluble bodies in the cell, including disrupting the recombinant cell, aggregating the insoluble bodies of the recombinant protein, and separating the resulting aggregate.

Abstract: An example information processing apparatus includes a processing system configured to control the information processing apparatus to: determine a base and a base direction in a virtual space; and determine whether an object can be generated in the virtual space relative to the base in the base direction. Based on determining that the object can be generated relative to the base in the base direction, an animated indicator may be displayed indicating at least the base direction for generating the object in the virtual space. Based on determining that a specified condition is satisfied, the object may be progressively generated relative to the base in the base direction.

Abstract: An image forming apparatus includes an image carrying member, a developing device, a cleaning unit and a processor. The developing device performs development with a developer containing a toner and a lubricant. The processor obtains information on a lubricant amount in the developing device; obtains, with respect to each section formed by dividing a surface of the image carrying member in a rotating shaft direction of the image carrying member, a partial coverage from electrostatic latent image writing information for a predetermined period about the section; sets, for the section having the partial coverage of a predetermined value or less, a developer supply amount for a patch to be formed outside an image forming region on the image carrying member, based on the above information; and causes the developing device to supply the set developer supply amount so that the patch is formed.

Abstract: An example information processing apparatus includes a processing system configured to control the information processing apparatus to: determine a base and a base direction in a virtual space; and determine whether an object can be generated in the virtual space relative to the base in the base direction. Based on determining that the object can be generated relative to the base in the base direction, an animated indicator may be displayed indicating at least the base direction for generating the object in the virtual space. Based on determining that a specified condition is satisfied, the object may be progressively generated relative to the base in the base direction.

Abstract: A piezoelectric element includes a piezoelectric body, an external electrode, and an internal electrode. The piezoelectric body includes first and second main surfaces opposing each other. The external electrode is disposed on the first main surface and has a first polarity. The internal electrode is disposed in the piezoelectric body to oppose the external electrode in a direction in which the first main surface and the second main surface oppose each other and has a second polarity different from the first polarity. A region from the internal electrode to the external electrode in the piezoelectric body is a polarizing region, and a region from the internal electrode to the second main surface in the piezoelectric body is a non-polarizing region.

Abstract: A non-limiting example game apparatus includes a display device, and a game screen is displayed on the display device. For example, a player character, a non-player character, a background object, etc. are displayed in the game screen. When finishing the drawing of a game image corresponding to the game screen, the smallest value of a Z value is acquired from a Z buffer. If the smallest value is less than a predetermined threshold value, when drawing a game image of a next frame, a target object that is near to a virtual camera is detected, and transparency of the target object that is nearer than a predetermined criterion is made to be increased.

Abstract: A water recovery method for improving water recovery efficiency may include inflowing a low concentration solution including water into an in-series flow path. The in-series flow path may include a plurality of flow paths for a low concentration solution coupled in series. The method may additionally include inflowing a high concentration solution having the same concentration into a plurality of flow paths for a high concentration solution. Each of the plurality of flow paths for the high concentration solution may be connected to each of plurality of flow paths for the low concentration solution via a respective semipermeable membrane being interposed therebetween.

Abstract: The present wet development device includes a developer carrying member, a supply member that supplies a liquid developer to the developer carrying member, a charger that charges the liquid developer on the developer carrying member, a cleaner that removes the liquid developer on the developer carrying member, and a bias applying member that applies alternating bias in contact with the developer carrying member before removing by the cleaner. In a direction in alignment with an axis of rotation of the developer carrying member, a range to which the alternating bias is applied by the bias applying, member is located within a range in which the liquid developer is supplied onto the developer carrying member.

Abstract: An image forming apparatus includes: a latent image bearing body on which a latent image is formed; a lubricant supplying unit configured to supply lubricant to the latent image bearing body; a developing unit configured to develop the latent image as a toner image by supplying the lubricant taken in from the latent image bearing body to the latent image bearing body together with charged toner; a transfer unit configured to transfer the toner image to an image bearing body at a transfer position by applying a transfer voltage to the toner image; and a control unit configured to control application of the transfer voltage, wherein the lubricant is charged to a polarity opposite to a charging polarity of the toner, and based on an area ratio of a transferred image and the number of transferred sheets, the control unit controls the transfer voltage to be applied.

Abstract: An image forming apparatus includes an image carrying member, a developing device, a cleaning unit and a processor. The developing device performs development with a developer containing a toner and a lubricant. The processor obtains information on a lubricant amount in the developing device; obtains, with respect to each section formed by dividing a surface of the image carrying member in a rotating shaft direction of the image carrying member, a partial coverage from electrostatic latent image writing information for a predetermined period about the section; sets, for the section having the partial coverage of a predetermined value or less, a developer supply amount for a patch to be formed outside an image forming region on the image carrying member, based on the above information; and causes the developing device to supply the set developer supply amount so that the patch is formed.

Abstract: An image forming apparatus includes: an image bearing body; a developing unit which develops an electrostatic latent image formed on the image bearing body as a toner image; a transfer unit which transfers the toner image to a receiver medium; a cleaning member which collects residual toner on the image bearing body; a lubricant supply unit which supplies lubricant onto the image bearing body; a measurement unit which measures a static frictional force generated between the image bearing body and the cleaning member; and a control unit which corrects the amount of lubricant on the image bearing body, wherein the control unit estimates a state of the lubricant on the image bearing body based on a change between a first and a second static frictional force, and based on the estimated state, the control unit selectively executes one of processing of supplying lubricant and processing of removing lubricant.

Abstract: Provided is a negative electrode for a nonaqueous secondary battery which achieves both suppression of reductive decomposition of an electrolyte or an electrolytic solution and suppression of an increase in resistance. Also provided are a method for producing such a negative electrode and a nonaqueous secondary battery employing such a negative electrode. A polymer coating layer is formed so as to coat at least part of surfaces of negative electrode active material particles containing silicon oxide (SiOx; 0.5?x?1.6). The polymer coating layer contains a cationic polymer having a positive zeta potential under neutral conditions. Since silicon oxide has a negative zeta potential, a thin uniform coating layer can be formed owing to Coulomb's force.

Abstract: A piezoelectric element includes a piezoelectric body, an external electrode, and an internal electrode. The piezoelectric body includes first and second main surfaces opposing each other. The external electrode is disposed on the first main surface and has a first polarity. The internal electrode is disposed in the piezoelectric body to oppose the external electrode in a direction in which the first main surface and the second main surface oppose each other and has a second polarity different from the first polarity. A region from the internal electrode to the external electrode in the piezoelectric body is a polarizing region, and a region from the internal electrode to the second main surface in the piezoelectric body is a non-polarizing region.

Abstract: An image forming apparatus includes: a latent image bearing body on which a latent image is formed; a lubricant supplying unit configured to supply lubricant to the latent image bearing body; a developing unit configured to develop the latent image as a toner image by supplying the lubricant taken in from the latent image bearing body to the latent image bearing body together with charged toner; a transfer unit configured to transfer the toner image to an image bearing body at a transfer position by applying a transfer voltage to the toner image; and a control unit configured to control application of the transfer voltage, wherein the lubricant is charged to a polarity opposite to a charging polarity of the toner, and based on an area ratio of a transferred image and the number of transferred sheets, the control unit controls the transfer voltage to be applied.

Abstract: An image forming apparatus includes: an image bearing body; a developing unit which develops an electrostatic latent image formed on the image bearing body as a toner image; a transfer unit which transfers the toner image to a receiver medium; a cleaning member which collects residual toner on the image bearing body; a lubricant supply unit which supplies lubricant onto the image bearing body; a measurement unit which measures a static frictional force generated between the image bearing body and the cleaning member; and a control unit which corrects the amount of lubricant on the image bearing body, wherein the control unit estimates a state of the lubricant on the image bearing body based on a change between a first and a second static frictional force, and based on the estimated state, the control unit selectively executes one of processing of supplying lubricant and processing of removing lubricant.

Abstract: An image forming apparatus includes: an image carrier; a development unit configured to develop, as a toner image, an electrostatic latent image formed on the image carrier; a transfer unit configured to transfer the toner image to a medium to which a toner image is to be transferred; a cleaning unit configured to recover toner remaining on the image carrier after transferring the toner image; a lubricant supply unit configured to supply lubricant on the image carrier; a charge unit disposed between the development unit and the cleaning unit, along a surface of the image carrier; and a control unit configured to perform a first mode and a second mode, the first mode configured to form the toner image, the second mode configured to allow the charge unit to increase the amount of charge on toner reaching the cleaning unit relative to that in the first mode.

Abstract: An image forming apparatus includes: an image carrier; a development unit configured to develop, as a toner image, an electrostatic latent image formed on the image carrier; a transfer unit configured to transfer the toner image to a medium to which a toner image is to be transferred; a cleaning unit configured to recover toner remaining on the image carrier after transferring the toner image; a lubricant supply unit configured to supply lubricant on the image carrier; a charge unit disposed between the development unit and the cleaning unit, along a surface of the image carrier; and a control unit configured to perform a first mode and a second mode, the first mode configured to form the toner image, the second mode configured to allow the charge unit to increase the amount of charge on toner reaching the cleaning unit relative to that in the first mode.

Abstract: Provided is a novel lithium silicate-based material useful as a positive electrode material for lithium ion secondary battery. The lithium silicate-based compound is represented by Li1.5FeSiO4.25. The lithium silicate-based compound is a compound including: lithium (Li); iron (Fe); silicon (Si); and oxygen (O), and expressed by a composition formula, Li1+2?FeSiO4+??c (?0.25???0.25, 0?c?0.5). The lithium silicate-based compound, of which iron (Fe) is trivalent, exerts a remarkable chemical stability as compared to Li2FeSiO4.

Abstract: The present wet development device includes a developer carrying member, a supply member that supplies a liquid developer to the developer carrying member, a charger that charges the liquid developer on the developer carrying member, a cleaner that removes the liquid developer on the developer carrying member, and a bias applying member that applies alternating bias in contact with the developer carrying member before removing by the cleaner. In a direction in alignment with an axis of rotation of the developer carrying member, a range to which the alternating bias is applied by the bias applying, member is located within a range in which the liquid developer is supplied onto the developer carrying member.

Abstract: A golf cart includes a frame, an adjuster, and a pair of suspension assemblies that reduce changes in camber angles in a pair of wheels caused by an adjustment of a ground clearance. The adjuster includes a pair of first adjusting portions and a pair of second adjusting portions. Each of the first adjusting portions includes a first upper hole and a first lower hole. Each of the second adjusting portions includes a second upper hole and a second lower hole. Each suspension assembly includes a damper and an arm portion. When the golf cart is set to a high ground clearance, the first adjusting portions are installed between the frame and the dampers, and the second adjusting portions are installed between the frame and the arm portions.