Abstract: Provided herein are genetically engineered Pichinde viruses that include three ambisense genomic segments. Two of the genomic segments include an additional coding region that may encode one or more swine influenza virus (SIV) proteins, such as a SIV hemagglutinin (HA) protein. SIV HA proteins can be from different subtypes of SIV. Also provided herein is a reverse genetics system for making genetically engineered Pichinde virus, and a collection of vectors that can be used to produce genetically engineered Pichinde virus. Further provided are methods for using a reverse genetics system, and methods for treating a SIV infection in a subject.

Abstract: The present invention provides a portable condensation-free temperature-adjustable radiant cooling board system. The system includes a supporting panel, a movable stand holding the supporting panel and an angle-adjuster. The supporting panel includes at least one water pipe layer, at least one insulation layer on one side of the at least one water pipe layer, at least one high emissivity radiative layer on the other side of the at least one water pipe layer and at least one low humidity layer in front of the at least one high emissivity radiative layer. The invented PRCB has high flexibility in the creation and adjustment of microthermal environments for diverse personal thermal comfort requirements, thus broaden the applicability of PRCSs in hot and humid climates.

Abstract: Provided herein are genetically engineered Pichinde viruses that include three ambisense genomic segments. The first genomic segment includes a coding region encoding a Z protein and a coding region encoding a L RdRp protein. The second genomic segment includes a coding region encoding a nucleoprotein (NP) and the third genomic segment includes a coding region encoding a glycoprotein. Each of the second and third genomic segments optionally include an additional coding region that may encode an antigen or a detectable marker. Also provided herein is a reverse genetics system for making a genetically engineered Pichinde virus, and a collection of vectors that can be used to produce a genetically engineered Pichinde virus. Further provided are methods for using a reverse genetics system, and methods for producing an immune response in a subject.

Abstract: Provided herein are genetically engineered Pichinde viruses that include three ambiense genomic segments. The first genomic segment includes two coding regions encoding a Z protein and a L RdRp protein. The second genomic segment includes a coding region encoding a nucleoprotein (NP) and the third genomic segment includes a coding region encoding a glycoprotein. Each of the second and third genomic segments include an additional coding region that may encode a reovirus sigma C protein or a reovims sigma B protein. In one embodiment, a genetically engineered Pichinde virus encodes a reovims sigma C protein and a reovirus sigma B protein. Also provided herein is a reverse genetics system for making genetically engineered Pichinde virus, and a collection of vectors that can be used to produce genetically engineered Pichinde virus. Further provided are methods for using a reverse genetics system, and methods for treating a reovirus infection in a subject.

Abstract: Provided herein is a SARS-CoV-2 coronavirus nucleocapsid (N) protein, where the N protein is substantially free of RNA. Also provided are methods of using the N protein including methods for inducing anti-N protein antibody in a subject, treating an infection, and detecting the presence or absence of anti-N protein antibody.

Abstract: Provided herein are genetically engineered Pichinde viruses that include three ambisense genomic segments. The first genomic segment includes a coding region encoding a Z protein and a coding region encoding a L RdRp protein. The second genomic segment includes a coding region encoding a nucleoprotein (NP) and the third genomic segment includes a coding region encoding a glycoprotein. Each of the second and third genomic segments optionally include an additional coding region that may encode an antigen or a detectable marker. Also provided herein is a reverse genetics system for making a genetically engineered Pichinde virus, and a collection of vectors that can be used to produce a genetically engineered Pichinde virus. Further provided are methods for using a reverse genetics system, and methods for producing an immune response in a subject.

Abstract: Provided herein are genetically engineered Pichinde viruses that include three ambisense genomic segments. The first genomic segment includes a coding region encoding a Z protein and a coding region encoding a L RdRp protein. The second genomic segment includes a coding region encoding a nucleoprotein (NP) and the third genomic segment includes a coding region encoding a glycoprotein. Each of the second and third genomic segments optionally include an additional coding region that may encode an antigen or a detectable marker. Also provided herein is a reverse genetics system for making a genetically engineered Pichinde virus, and a collection of vectors that can be used to produce a genetically engineered Pichinde virus. Further provided are methods for using a reverse genetics system, and methods for producing an immune response in a subject.

Abstract: A thermal detection device includes a first camera, a second camera, a thermal camera, and a processor. The thermal camera is disposed between the first camera and the second camera, and detects a to-be-measured object to generate a first temperature value. The processor includes a control unit, which controls the first camera and the second camera according to settings of a first measurement mode and a second measurement mode. In the first measurement mode, the control unit captures images from the first camera and the second camera to measure a first distance between the to-be-measured object and an installation surface. In the second measurement mode, the control unit captures images from the first camera to measure a size of the to-be-measured object. The processor determines a second temperature value of the to-be-measured object according to the first temperature value, the first distance, and the size of the to-be-measured object.

Abstract: A thermal detection device includes a first camera, a second camera, a thermal camera, and a processor. The thermal camera is disposed between the first camera and the second camera, and detects a to-be-measured object to generate a first temperature value. The processor includes a control unit, which controls the first camera and the second camera according to settings of a first measurement mode and a second measurement mode. In the first measurement mode, the control unit captures images from the first camera and the second camera to measure a first distance between the to-be-measured object and an installation surface. In the second measurement mode, the control unit captures images from the first camera to measure a size of the to-be-measured object. The processor determines a second temperature value of the to-be-measured object according to the first temperature value, the first distance, and the size of the to-be-measured object.

Abstract: Provided herein are genetically engineered Pichinde viruses that include three ambisense genomic segments. The first genomic segment includes a coding region encoding a Z protein and a coding region encoding a L RdRp protein. The second genomic segment includes a coding region encoding a nucleoprotein (NP) and the third genomic segment includes a coding region encoding a glycoprotein. Each of the second and third genomic segments optionally include an additional coding region that may encode an antigen or a detectable marker. Also provided herein is a reverse genetics system for making a genetically engineered Pichinde virus, and a collection of vectors that can be used to produce a genetically engineered Pichinde virus. Further provided are methods for using a reverse genetics system, and methods for producing an immune response in a subject.