Abstract: Methods and system that determines disulfide and trisulfide linkages within analytes (e.g., polypeptides) is described. In certain aspects. a sample comprising polypeptides (such as an antibody) may be subjected to dissociation using an electron activated dissociation (which can include electron capture dissociation and electron transfer dissociation) and the fragmentated portions are analyzed using a mass spectrometer to produce a spectrum. The spectrum is analyzed by a processor to identify peaks from the spectrum that are related to one another in the spectra by a separation of 32 mass units. In identifying an antibody comprising peptide segments linked via a trisulfide bond. for example, four different peaks representing two different peptides are searched for and identified representing a first peptide portion having mass/charge of A and A+32 and a second peptide having mass/charge of B and B+32.

Abstract: The present invention provides a refrigerating and freezing device, including a cabinet and a door body provided on the cabinet for opening or closing the cabinet, wherein the door body includes a first door body and a second door body spaced apart from each other. In a closed state, the first door body and the second door body are located on the same plane. The first door body is provided with a sound generating device on a side facing the second door body, and the direction of the sound emitted by the sound generating device faces the second door body. A gap serving as a sound mixing chamber is formed between the first door body and the second door body. Therefore, the sound emitted by the sound generating device can be reflected back and forth in the sound mixing chamber, thereby making the sound more pleasant and vigorous.

Abstract: Process for direct coal liquefaction of coal, including: (1) preparing a coal slurry from raw coal; (2) preheating the coal slurry, then feeding it into reaction system to undergo liquefaction reaction; (3) separating reaction products in a separator to form a liquid phase and a gas phase, wherein the liquid phase is fractionated in an atmospheric tower into a light oil fraction and a bottom product; (4) feeding the atmospheric tower bottom product to a vacuum tower to separate into distillate and vacuum residue; (5) mixing the light oil fraction and the distillate to form a mixture, then feeding the mixture to a suspended bed hydrotreating reactor with forced circulation for hydrogenation; (6) fractionating hydrogenation products into oil products and a hydrogen donor recycling solvent. The process can operate long periods, with higher reactor efficiency and utilization factor, increased liquid oil yield and can supply high-quality feedstock for further processing.

Abstract: Process for direct coal liquefaction of coal, including: (1) preparing a coal slurry from raw coal; (2) preheating the coal slurry, then feeding it into reaction system to undergo liquefaction reaction; (3) separating reaction products in a separator to form a liquid phase and a gas phase, wherein the liquid phase is fractionated in an atmospheric tower into a light oil fraction and a bottom product; (4) feeding the atmospheric tower bottom product to a vacuum tower to separate into distillate and vacuum residue; (5) mixing the light oil fraction and the distillate to form a mixture, then feeding the mixture to a suspended bed hydrotreating reactor with forced circulation for hydrogenation; (6) fractionating hydrogenation products into oil products and a hydrogen donor recycling solvent. The process can operate long periods, with higher reactor efficiency and utilization factor, increased liquid oil yield and can supply high-quality feedstock for further processing.

Abstract: Process for direct coal liquefaction of coal, including: (1) preparing a coal slurry from raw coal; (2) preheating the coal slurry, then feeding it into reaction system to undergo liquefaction reaction; (3) separating reaction products in a separator to form a liquid phase and a gas phase, wherein the liquid phase is fractionated in an atmospheric tower into a light oil fraction and a bottom product; (4) feeding the atmospheric tower bottom product to a vacuum tower to separate into distillate and vacuum residue; (5) mixing the light oil fraction and the distillate to form a mixture, then feeding the mixture to a suspended bed hydrotreating reactor with forced circulation for hydrogenation; (6) fractionating hydrogenation products into oil products and a hydrogen donor recycling solvent. The process can operate long periods, with higher reactor efficiency and utilization factor, increased liquid oil yield and can supply high-quality feedstock for further processing.