Abstract: Provided is a botulinum toxin producing method which is simple achieves a high toxin yield, and obtains a toxin having high specific activity. This botulinum toxin producing method includes: (A) a step in which a botulinum toxin is produced from botulinum toxin-producing bacteria in a medium, and a mixture a is obtained which contains a botulinum toxin, a bacterial component, and a nucleic acid component derived from the botulinum toxin; (B) a step in which the mixture a is subjected to the removal of the bacterial component, and a mixture b is obtained which contains a nucleic acid component and a botulinum toxin; (C) a step in which an endonuclease is added to the mixture b and a mixture c is obtained which contains a nucleic acid degradation product and a botulinum toxin; and (D) a step in which the mixture c is subjected to removal of the nucleic acid degradation product, and an isolated botulinum toxin liquid d is obtained.

Abstract: Provided is a botulinum toxin producing method which is simple achieves a high toxin yield, and obtains a toxin having high specific activity. This botulinum toxin producing method includes: (A) a step in which a botulinum toxin is produced from botulinum toxin-producing bacteria in a medium, and a mixture a is obtained which contains a botulinum toxin, a bacterial component, and a nucleic acid component derived from the botulinum toxin; (B) a step in which the mixture a is subjected to the removal of the bacterial component, and a mixture b is obtained which contains a nucleic acid component and a botulinum toxin; (C) a step in which an endonuclease is added to the mixture b and a mixture c is obtained which contains a nucleic acid degradation product and a botulinum toxin; and (D) a step in which the mixture c is subjected to removal of the nucleic acid degradation product, and an isolated botulinum toxin liquid d is obtained.

Abstract: A GPS receiver has a plurality of data demodulator circuits assigned to a plurality of GPS satellites, respectively, and a data memory which stores detailed orbit information of the GPS satellites. The GPS receiver searches for satellites the detailed orbit information of which was stored in the memory previously. When these searched GPS satellites are acquired, the GPS receiver executes satellite frame synchronization determination processing to check for an agreement among predetermined data included in satellite data transmitted from the acquired GPS satellites. When the searched GPS satellites cannot be acquired or the predetermined data do not agree, the GPS receiver executes a single-satellite frame synchronization determination processing to check for an agreement of predetermined data included in a plurality of frames of the satellite data. The GPS receiver starts positioning processing in response to an establishment of agreement to calculate its position.

Abstract: Disclosed is a disc case characterized in that it allows both loading of the disc as housed in the case main body to the disc drive section and loading of the disc as a simple to the disc drive section, that it is produced with the reduced number of parts and reduced number of assembly steps so as to achieve cost reduction and enjoys extended life and high reliability, and that the opening and closing handleability of the cover is highly facilitated; the disc case comprising a rectangular case main body, having a full-face opening for fitting a data recording disc therethrough, and another opening defined at the bottom through which the data recording surface of the disc can radially be exposed; a cover plate which can close the full-face opening of the case main body; a couple of first engagement pieces, provided on the rear surface of the cover plate; a couple of second engagement pieces, provided in the case main body which resiliently engage with the first engagement pieces to lock the cover plate as clos

Abstract: The method is characterized by bringing a mixture of ammonia and air to a temperature substantially equal to the temperature of the waste flue gas in the flue at the exit of an air heater, and then injecting the mixture into the flue from a number of nozzles arranged in uniformly spaced relation in the flue while regulating the respective flow rates thereof.