Abstract: Provided are a packing material used for size exclusion chromatography, which has high alkali resistance and suppressed non-specific adsorption, and a method for producing the same. A packing material, wherein one end of a block copolymer represented by the following formula 1 is bonded to a porous organic polymer carrier containing 60 to 95 mol % of a repeating unit derived from glycidyl methacrylate and 5 to 40 mol % of a repeating unit derived from a polyfunctional monomer via an ether bond derived from a terminal hydroxyl group, HO—(C2H4O)l—(C3H6O)m—(C2H4O)n—H??(formula 1) wherein l is an integer of 5 to 140, m is an integer of 15 to 75, and n is an integer of 5 to 140, and a proportion of m to a sum of l, m, and n is 5 to 85%.

Abstract: A packing material, wherein to a porous organic polymer carrier including 60 to 95 mol % of a repeating unit derived from glycidyl methacrylate and 5 to 40 mol % of a repeating unit derived from a polyfunctional monomer, one end of at least one alkylene group selected from a linear alkylene group, a cycloalkylene group, and a linear alkylcycloalkylene group, having 4 to 9 carbon atoms is bonded by a glycidyl group derived from glycidyl methacrylate, and an other end of the alkylene group is bonded to any one end of a polyol via an ether bond.

Abstract: A packing material, wherein to a porous organic polymer carrier including 60 to 95 mol % of a repeating unit derived from glycidyl methacrylate and 5 to 40 mol % of a repeating unit derived from a polyfunctional monomer, one end of at least one alkylene group selected from a linear alkylene group, a cycloalkylene group, and a linear alkylcycloalkylene group, having 4 to 9 carbon atoms is bonded by a glycidyl group derived from glycidyl methacrylate, and an other end of the alkylene group is bonded to any one end of a polyol via an ether bond.

Abstract: The present invention relates to a method of separating and analyzing a mixture of oligonucleotides, including performing liquid chromatography using a column packed with a packing material obtained by fixing a diol to a surface of each of porous particles formed of a crosslinked organic polymer. According to this method, the oligonucleotides can be separated and analyzed with higher sensitivity compared to cases where columns having silica gel as a base material are used. In addition, the column can be washed with an alkaline solution.

Abstract: The present invention relates to a method of separating and analyzing a mixture of oligonucleotides, including performing liquid chromatography using a column packed with a packing material obtained by fixing a diol to a surface of each of porous particles formed of a crosslinked organic polymer. According to this method, the oligonucleotides can be separated and analyzed with higher sensitivity compared to cases where columns having silica gel as a base material are used. In addition, the column can be washed with an alkaline solution.

Abstract: An object of the present invention is to obtain a protein monomer useful as a raw material for medicines industrially and economically in high yield and high purity. In the method for purifying a protein of the present invention, a protein solution containing a protein monomer and a protein aggregate is passed through a column holding a porous rigid polymeric self-supporting structure to which a hydrophobic group is immobilized, and then recovering the protein monomer in a flow-through mode.

Abstract: Provided is a method of removing a protein aggregate which can obtain a useful protein monomer in a high yield and a high purity as a raw material of a pharmaceutical product or the like. The method of removing a protein aggregate includes: a step of making the monomer of a protein and the aggregate of proteins adsorb to the column by making a solution containing a monomer of a protein and an aggregate of proteins pass through a column containing a hard porous polymer self-supporting structure to which a strong cation exchange group is fixed; and a step of making a mobile phase consisting of a mixed solution of a buffer solution and an ionic buffer solution pass through the column to which the monomer of a protein and the aggregate of proteins are adsorbed, to selectively elute the monomer of a protein.

Abstract: A method of manufacturing 1,4-butanediol through acetyl-CoA, acetoacetyl-CoA, 3-hydroxybutyryl-CoA, crotonyl-CoA, and 4-hydroxybutyryl-CoA by using a microbe and/or a culture thereof, wherein the microbe in the manufacturing method for 1,4-butanediol includes any one of genes among (a) a gene that has a base sequence of sequence number 1, (b) a gene that has a base sequence such that one or more bases are deleted, substituted, or added in a base sequence of sequence number 1, wherein the gene has a base sequence with an identity greater than or equal to 90% with respect to the base sequence of sequence number 1, and (c) a gene that hybridizes with a gene that has a base sequence complementary with a gene that has a base sequence described in sequence number 1 on a stringent condition, and includes any one or more genes among (d) genes that have base sequences of sequence numbers 2 to 9, (e) genes that have base sequences such that one or more bases are deleted, substituted, or added in base sequences of seque

Abstract: A method of manufacturing 1,4-butanediol through acetyl-CoA, acetoacetyl-CoA, 3-hydroxybutyryl-CoA, crotonyl-CoA, and 4-hydroxybutyryl-CoA by using a microbe and/or a culture thereof, wherein the microbe in the manufacturing method for 1,4-butanediol includes any one of genes among (a) a gene that has a base sequence of sequence number 1, (b) a gene that has a base sequence such that one or more bases are deleted, substituted, or added in a base sequence of sequence number 1, wherein the gene has a base sequence with an identity greater than or equal to 90% with respect to the base sequence of sequence number 1, and (c) a gene that hybridizes with a gene that has a base sequence complementary with a gene that has a base sequence described in sequence number 1 on a stringent condition, and includes any one or more genes among (d) genes that have base sequences of sequence numbers 2 to 9, (e) genes that have base sequences such that one or more bases are deleted, substituted, or added in base sequences of seque

Abstract: A method of manufacturing 1,4-butanediol, using a microbe and/or a culture thereof, by an enzyme reaction system that uses acyl-CoA reductase, via 3-hydroxybutyryl-CoA, crotonyl-CoA and 4-hydroxybutyryl CoA in this order, wherein the reactivity of the acyl-CoA reductase to 4-hydroxybutyryl CoA is greater than or equal to 0.05 times of the reactivity of the acyl-CoA reductase to 3-hydroxybutyryl-CoA.

Abstract: A method of manufacturing a butanediol through 3-hydroxybutyryl-CoA that uses a microbe and/or a culture thereof and utilizes an enzyme reaction that is caused by an acyl-CoA reductase, wherein the microbe in the manufacturing method for a butanediol is characterized in that an activity of an acyl-CoA hydratase (EC number: 3. 1. 2. ?) is deleted or reduced.

Abstract: A method of manufacturing 1,4-butanediol by an enzyme reaction system via 3-hydroxybutyryl-CoA, crotonyl-CoA and 4-hydroxybutyryl CoA, in this order, using a microbe and/or a culture thereof, wherein the 3-hydroxybutyryl-CoA is an optically active substance, and wherein the microbe includes (1) a gene that codes enoyl-CoA hydratase, (2) a gene that codes vinylacetyl-CoA delta-isomerase, (3) a gene that codes 4-hydroxybutyryl CoA dehydratase, and (4) a gene that codes acyl-CoA reductase whose substrate specificity has an optical selectivity opposite to that of the 3-hydroxybutyryl-CoA.

Abstract: A method of manufacturing 1,4-butanediol, using a microbe and/or a culture thereof, by an enzyme reaction system that uses acetoacetyl-CoA reductase and enoyl-CoA hydratase, via acetoacetyl-CoA, 3-hydroxybutyryl-CoA and crotonyl-CoA in this order, wherein each of the acetoacetyl-CoA reductase and the enoyl-CoA hydratase is specific to a stereoisomer of 3-hydroxybutyryl-CoA.

Abstract: A method of manufacturing a butanediol includes a step of culturing a microbe that contains a gene that codes acetoacetyl-CoA synthase that catalyzes a reaction that irreversibly produces acetoacetyl-CoA from acetyl-CoA and malonyl-CoA, acetoacetyl-CoA reductase that catalyzes a reaction that produces 3-hydroxybutyryl-CoA from acetoacetyl-CoA, and an enzyme that catalyzes a reaction for producing a butanediol from 3-hydroxybutyryl-CoA.

Abstract: A gene is selected from the group consisting of a gene (a) that has a base sequence of sequence number 1, a gene (b) that has a base sequence such that one or more bases are deleted, substituted, or added in a base sequence of sequence number 1, wherein the gene (b) has a base sequence with an identity greater than or equal to 90% with respect to the base sequence of sequence number 1, and a gene (c) that hybridizes with a gene that has a base sequence complimentary with a gene that has a base sequence of sequence number 1 on a stringent condition, wherein the selected gene in combination with a gene that codes enoyl-CoA hydratase is able to provide a microbe or a culture of the microbe with an ability to convert 3-hydroxybutyryl-CoA into 4-hydroxybutyryl-CoA or 4-hydroxybutyryl-CoA into 3-hydroxybutyryl-CoA.