Abstract: The present invention relates to a composition comprising a solid carrier, a capture moiety, a functional protein, a first linker connecting the capture moiety to the solid carrier, a second linker connecting the functional protein to the capture moiety, a first protective layer fully embedding the solid carrier, fully or partially embedding the first linker, and not or partially embedding the capture moiety, a second protective layer fully or partially embedding the capture moiety, fully embedding the second linker, and fully or partially embedding the functional protein.

Abstract: A method of producing nanobiocatalysts includes: suspending a support material in a liquid medium; functionalizing a surface of the support material by mixing the suspended support material with a functionalizer for a first predefined time at a first predefined temperature, wherein a first broth results; applying a protein directly in the first broth after the first predefined time; mixing the protein with the first broth and incubating the protein for a second predefined time at a second predefined temperature, wherein a second broth results; immobilizing the incubated protein onto the functionalized surface of the support material, wherein a third broth results; filtering the third broth; and gathering a retentate of the filtering. By directly processing the first and second broths allows for providing a continuous process and production of the nanobiocatalysts, which can be optimized regarding time, water, chemical and energy consumption. Also, problems related to thixotrophy can be minimized.

Abstract: A method of producing nanobiocatalysts includes: suspending a support material in a liquid medium; functionalizing a surface of the support material by mixing the suspended support material with a functionalizer for a first predefined time at a first predefined temperature, wherein a first broth results; applying a protein directly in the first broth after the first predefined time; mixing the protein with the first broth and incubating the protein for a second predefined time at a second predefined temperature, wherein a second broth results; immobilizing the incubated protein onto the functionalized surface of the support material, wherein a third broth results; filtering the third broth; and gathering a retentate of the filtering. By directly processing the first and second broths allows for providing a continuous process and production of the nanobiocatalysts, which can be optimized regarding time, water, chemical and energy consumption. Also, problems related to thixotrophy can be minimized.

Abstract: A device is provided for carrying out optical measurements such as fluorescence and absorbance measurements on samples distributed at measurement sites on a support, including first and second illumination apparatus, an optical detector, first and second optical fibres with an end facing the measurement sites at first and second faces of the support, respectively, which device further includes optical configuration apparatus configurable so as to allow the following configurations: (i) optical coupling of the first illumination apparatus with the first optical fibres and of the optical detector with the second optical fibres, (ii) optical coupling of the second illumination apparatus and the optical detector with the second optical fibres, and/or (iii) optical coupling of the second illumination apparatus and the optical detector with the first optical fibres. A corresponding method implemented in this device is also provided.